Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate

Kuc, Vanesa; Vázquez, Susana; Hernández, Edgardo A.; Martínez-Alvarez, Lucas M.; Primitz, Julia Villalba; Cormack, Walter P. Mac; Ruberto, Lucas

doi:10.1007/s00300-019-02508-1

Cited by 8 publications

(2 citation statements)

References 41 publications

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“…Their popularity can be accredited to their capacity to unravel the molecular mechanisms underpinning a process of interest (Jung et al, 2011;Rio, 2014;Jurelevicius et al, 2021;Méndez García and García de Llasera, 2021;Ramírez-Fernández et al, 2021;van Dorst et al, 2021). In the context of hydrocarbon bioremediation, the primary focus in the use of these technologies has been investigating the mechanisms driving alkane and PAH degradation (Jurelevicius et al, 2012;Tribelli et al, 2018;Kuc et al, 2019). When assessing the capacity of a microbiome to degrade diesel the relative abundance of a gene called alkB which encodes the alkane monooxygenase enzyme is used (Yergeau et al, 2012;Crane et al, 2018;Li et al, 2020;Ling et al, 2023).…”

Section: Omic Tools In Bioremediation Researchmentioning

confidence: 99%

“…Alkane monooxygenase is the first enzyme in the pathway responsible for the terminal oxidation of alkanes and has been commonly found in hydrocarbon contaminated soil in both cold and temperate climates (Yergeau et al, 2012;Crane et al, 2018;Li et al, 2020;Ling et al, 2023). Within the context of bioremediation research, an increase in alkB is thought to be associated with the degradation of short chain alkanes, which form a large proportion by mass of Antarctic blend diesels (Yergeau et al, 2012;Kuc et al, 2019;Semenova et al, 2022). Within hydrocarbon contaminated soils it is not uncommon to find an alkB relative abundance of greater than 100 gene copies detected per 100 organisms, likely due to many organisms having multiple copies of this gene (Yergeau et al, 2012).…”

Section: Omic Tools In Bioremediation Researchmentioning

confidence: 99%

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Utilization of—Omic technologies in cold climate hydrocarbon bioremediation: a text-mining approach

2023

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Hydrocarbon spills in cold climates are a prominent and enduring form of anthropogenic contamination. Bioremediation is one of a suite of remediation tools that has emerged as a cost-effective strategy for transforming these contaminants in soil, ideally into less harmful products. However, little is understood about the molecular mechanisms driving these complex, microbially mediated processes. The emergence of −omic technologies has led to a revolution within the sphere of environmental microbiology allowing for the identification and study of so called ‘unculturable’ organisms. In the last decade, −omic technologies have emerged as a powerful tool in filling this gap in our knowledge on the interactions between these organisms and their environment in vivo. Here, we utilize the text mining software Vosviewer to process meta-data and visualize key trends relating to cold climate bioremediation projects. The results of text mining of the literature revealed a shift over time from optimizing bioremediation experiments on the macro/community level to, in more recent years focusing on individual organisms of interest, interactions within the microbiome and the investigation of novel metabolic degradation pathways. This shift in research focus was made possible in large part by the rise of omics studies allowing research to focus not only what organisms/metabolic pathways are present but those which are functional. However, all is not harmonious, as the development of downstream analytical methods and associated processing tools have outpaced sample preparation methods, especially when dealing with the unique challenges posed when analyzing soil-based samples.

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Section: Omic Tools In Bioremediation Researchmentioning

confidence: 99%

Section: Omic Tools In Bioremediation Researchmentioning

confidence: 99%

Utilization of—Omic technologies in cold climate hydrocarbon bioremediation: a text-mining approach

2023

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Microbial associations for bioremediation. What does “microbial consortia” mean?

Massot

Bernard

Álvarez

et al. 2022

Appl Microbiol Biotechnol

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Bioremediation of hydrocarbon-contaminated soil from Carlini Station, Antarctica: effectiveness of different nutrient sources as biostimulation agents

et al. 2021

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Logistics and scientific activities carried out in Antarctic stations entail the risk of contamination by fuels.Among remediation strategies, biostimulation of chronically contaminated Antarctic soils significantly improves the efficiency of hydrocarbons (HCs) removal. The aim of this study was to evaluate the performance of different nutrient formulations as biostimulation agents, in order to improve the elimination of diesel fuel from Antarctic soils, in both oxic and anoxic conditions. A field test was performed in microcosms (15 kg of soil each) as experimental systems. Each microcosm was prepared by triplicate, sampled every 10 days over a 50-days period and sampled again one year later. Changes in bacterial communities, and qualitative and quantitative HCs analysis were determined. Our results showed that, during the early stages of the process, a multi-component commercial product like OSEII ® (containing nutrients, enzymes and surfactants) determines a rapid elimination of HCs with changes in the structure of the bacterial soil community, whereas a more cost-effective slow-release fertilizer like Nitrofoska ® would be efficient in a long-term bioremediation process.

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Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate

Abstract: Hydrocarbon-contaminated Antarctic soil: changes in bacterial community structure during the progress of enrichment cultures with different n-alkanes as substrate

Cited by 8 publications

References 41 publications

Utilization of—Omic technologies in cold climate hydrocarbon bioremediation: a text-mining approach

Utilization of—Omic technologies in cold climate hydrocarbon bioremediation: a text-mining approach

Microbial associations for bioremediation. What does “microbial consortia” mean?

Bioremediation of hydrocarbon-contaminated soil from Carlini Station, Antarctica: effectiveness of different nutrient sources as biostimulation agents

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