Hydrodynamic Characterization of Physicochemical Process in Stirred Tanks and Agglomeration Reactors

Oyegbile, Benjamin A.; Akdogan, G.

doi:10.5772/intechopen.77014

Cited by 5 publications

(2 citation statements)

References 97 publications

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“…Recently, a transparent 15 000 L cell culture reactor has been used to characterize the occurring flow patterns with the benefit of optical access into the reactor . Computational fluid dynamics (CFD) simulations have also been widely used to characterize the flow fields of large bioreactors and provide information of the mixing dynamics in these reactors . Based on these simulations, the time which a cell spends in a different microenvironment can be estimated and in turn be used to design scale‐down simulators .…”

Section: Introductionmentioning

confidence: 99%

Scale‐down simulators for mammalian cell culture as tools to access the impact of inhomogeneities occurring in large‐scale bioreactors

Paul

Herwig

2020

Engineering in Life Sciences

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During the scale-up of a bioprocess, not all characteristics of the process can be kept constant throughout the different scales. This typically results in increased mixing times with increasing reactor volumes. The poor mixing leads in turn to the formation of concentration gradients throughout the reactor and exposes cells to varying external conditions based on their location in the bioreactor. This can affect process performance and complicate process scale-up. Scale-down simulators, which aim at replicating the large-scale environment, expose the cells to changing environmental conditions. This has the potential to reveal adaptation mechanisms, which cells are using to adjust to rapidly fluctuating environmental conditions and can identify possible root causes for difficulties maintaining similar process performance at different scales. This understanding is of utmost importance in process validation. Additionally, these simulators also have the potential to be used for selecting cells, which are most robust when encountering changing extracellular conditions. The aim of this review is to summarize recent work in this interesting and promising area with the focus on mammalian bioprocesses, since microbial processes have been extensively reviewed. K E Y W O R D S 2-compartment system, gradients, inhomogeneity, large-scale bioprocess, scale-upAbbreviations: CFD, computational fluid dynamics; CHO, Chinese Hamster ovary; CS, compartment system; PFR, plug flow reactor; STR, stirred tank reactor; VCC, viable cell count.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. throughput and decrease time lines for process development and characterization [1]. Particularly production processes for monoclonal antibodies (mAbs) that are in high demand, like adalimumab (Humira TM ) [2], are transferred to large-scale production reactors. These bioreactors can reach volumes of up to 25 000 L for mammalian cell culture processes [3]. Furthermore, it has been estimated that approximately 50% of the biologics will continue to be produced in bioreactor volumes of at least 5000 L [4]. There are different approaches Eng Life Sci.

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Section: Introductionmentioning

confidence: 99%

Scale‐down simulators for mammalian cell culture as tools to access the impact of inhomogeneities occurring in large‐scale bioreactors

Paul

Herwig

2020

Engineering in Life Sciences

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“…They reported that at that time, although many studies on bulk flow patterns had already been published, these were primarily at the laboratory scale, and few reported multiphase modeling at either the laboratory scale or at full scale. Recently, Oyegbile and Akdogan (2018) compiled and reviewed the state of the art in the experimental and CFD characterization of stirred tanks and agglomeration reactors. In what follows, we review some of the recent CFD studies in this field.…”

Section: Introductionmentioning

confidence: 99%

Development of an oscillation-based technology for the removal of colloidal particles from water: CFD modeling and experiments

Halfi

Arad

Brenner

et al. 2020

Engineering Applications of Computational Fluid Mechanics

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Colloidal particles removal from water is a challenge in surface water treatment. Previously, we suggested an original technique for colloidal particles separation from water based on physical flow manipulation by an oscillating device. Laboratory experiments and 2D numerical simulation indicated that this technology enhances colloidal particles grouping and enables their rapid, simultaneous aggregation and sedimentation. The 2D model used in that study was unable to fully elucidate the grouping mechanism, settling pattern, or particle trajectories. Here, we extended the numerical simulation from 2D to 3D and examined the system's distinctive flow field. The flow field was solved with computational fluid dynamics (CFD) simulations in the commercial ANSYS Fluent code and validated by dye injection experiments. The sedimentation patterns could nicely be explained by the computational results. This explanation was strengthened by two-dimensional population balance model simulations, which showed that particles aggregated in two zones trailing the paddle edges. Additionally, the results indicate significantly higher TKE values and faster upward vertical velocities at the higher oscillating frequency, which explains the different sedimentation patterns and removal efficiencies generated by the different oscillation frequencies. The use of 3D numerical simulations will help to better understand and further optimize this novel technology. ARTICLE HISTORY

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Mixing studies in an unbaffled bioreactor using a computational model corroborated with in-situ Raman and imaging analyses

Vasudevan

Eka

Chew

2022

Chemical Engineering Journal Advances

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Hydrodynamic Characterization of Physicochemical Process in Stirred Tanks and Agglomeration Reactors

Cited by 5 publications

References 97 publications

Scale‐down simulators for mammalian cell culture as tools to access the impact of inhomogeneities occurring in large‐scale bioreactors

Scale‐down simulators for mammalian cell culture as tools to access the impact of inhomogeneities occurring in large‐scale bioreactors

Development of an oscillation-based technology for the removal of colloidal particles from water: CFD modeling and experiments

Mixing studies in an unbaffled bioreactor using a computational model corroborated with in-situ Raman and imaging analyses

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