Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

Mogha, Pankaj; Srivastava, Ankita; Kumar, Sushant; Das, Somnath; Kureel, Sanjay Kumar; Dwivedi, Alka; Karulkar, Atharva; Jain, Nikita; Sawant, Abhijeet; Narasimhan, Chitra; Majumder, Abhijit; Purwar, Rahul

doi:10.1039/c9ra00781d

Cited by 12 publications

(8 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our earlier work, we have demonstrated that soft substrate can delay the onset of replicative senescence in BM-hMSCs [7], which was later supported by observations from other groups as well [16]. We demonstrated the same with patient derived keratinocytes as well [8].…”

Section: Discussionsupporting

confidence: 83%

“…However, out of the four ECMs tested, gelatin showed the best result in terms of PD/passage and final CPD before growth arrest. While cells on TCP had 2 8 times increase in total cell number, cells on gelatin coated plastic had 2 14 times increase in number before they reached growth arrest. However, it should be noted that the cells entered growth arrest at the same time irrespective of coating indicating no effect of the ECM on the culture duration at which growth arrest happens.…”

Section: Ecm Protein Coating Enhances the Proliferation Rate Of Mesenchymal Stem Cells In Vitromentioning

confidence: 97%

“…After four hours of cell seeding, at least 15 images were acquired from each flask sampling the entire culture area. The population doubling for each passage were calculated as previously described [7,8]. Briefly, total number of cells per frame were manually counted and divided by the total frame area (cm 2 ) to get the seeding density.…”

Section: Pd and Cpd Calculationmentioning

confidence: 99%

See 2 more Smart Citations

Extracellular Matrix protein gelatin provides higher expansion, reduces size heterogeneity, and maintains cell stiffness in a long-term culture of mesenchymal stem cells

Mogha

Iyer

Majumder

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Extracellular matrices (ECM) present in our tissues play a significant role in maintaining tissue homeostasis through various physical and chemical cues such as topology, stiffness, and secretion of biochemicals. They are known to influence the behaviour of resident stem cells. It is also known that ECM type and coating density on cell culture plates strongly influence in vitro cellular behaviour. However, the influence of ECM protein coating on long term mesenchymal stem cell expansion has not been studied yet. To address this gap, we cultured bone-marrow derived hMSCs for multiple passages on the tissue culture plastic plates coated with 25 μg/ml of various ECM proteins. We found that cells on plates coated with ECM proteins had much higher proliferation compared to the regular tissue culture plates. Further, gelatin coated plates helped the cells to grow faster compared to collagen, fibronectin, and laminin coated plates. Additionally, the use of Gelatin showed less size heterogeneity among the cells when expanded from passages P3 to P9. Gelatin also helped in maintaining cellular stiffness which was not observed across other ECM proteins. In summary, in this research, we have shown that gelatin which is the least expensive compared to other ECM proteins provides a better platform for mesenchymal stem cell expansion.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Ecm Protein Coating Enhances the Proliferation Rate Of Mesenchymal Stem Cells In Vitromentioning

confidence: 97%

Section: Pd and Cpd Calculationmentioning

confidence: 99%

See 1 more Smart Citation

Extracellular Matrix protein gelatin provides higher expansion, reduces size heterogeneity, and maintains cell stiffness in a long-term culture of mesenchymal stem cells

Mogha

Iyer

Majumder

2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The migration activity of the cell was influenced by the presence of integrin and matrix metalloproteinases expression. Besides, a study on the polyacrylamide gel coated with collagen I indicate the KCs respond to IFN Ƴ responsive genes (HLA DR and CXCL10) [ 132 ]. The presence of these genes promoted the excellent expansion of KCs for wound healing.…”

Section: In Vivo and In Vitro Studiesmentioning

confidence: 99%

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

Masri

Mazlan

Zulkiflee

et al. 2022

IJMS

View full text Add to dashboard Cite

Skin substitutes can provide a temporary or permanent treatment option for chronic wounds. The selection of skin substitutes depends on several factors, including the type of wound and its severity. Full-thickness skin grafts (SGs) require a well-vascularised bed and sometimes will lead to contraction and scarring formation. Besides, donor sites for full-thickness skin grafts are very limited if the wound area is big, and it has been proven to have the lowest survival rate compared to thick- and thin-split thickness. Tissue engineering technology has introduced new advanced strategies since the last decades to fabricate the composite scaffold via the 3D-bioprinting approach as a tissue replacement strategy. Considering the current global donor shortage for autologous split-thickness skin graft (ASSG), skin 3D-bioprinting has emerged as a potential alternative to replace the ASSG treatment. The three-dimensional (3D)-bioprinting technique yields scaffold fabrication with the combination of biomaterials and cells to form bioinks. Thus, the essential key factor for success in 3D-bioprinting is selecting and developing suitable bioinks to maintain the mechanisms of cellular activity. This crucial stage is vital to mimic the native extracellular matrix (ECM) for the sustainability of cell viability before tissue regeneration. This comprehensive review outlined the application of the 3D-bioprinting technique to develop skin tissue regeneration. The cell viability of human skin cells, dermal fibroblasts (DFs), and keratinocytes (KCs) during in vitro testing has been further discussed prior to in vivo application. It is essential to ensure the printed tissue/organ constantly allows cellular activities, including cell proliferation rate and migration capacity. Therefore, 3D-bioprinting plays a vital role in developing a complex skin tissue structure for tissue replacement approach in future precision medicine.

show abstract

“…Healthy skin specimens were collected from plastic surgery department of B.Y.L. Nair Charitable Hospital, Mumbai, upon approval of the Institute Ethics Committee (IEC) of IIT described earlier after obtaining the written, informed consent (Das et al, 2019, Mogha et al, 2019, Purwar et al, 2006 .…”

Section: Human Primary Keratinocyte Isolation and Culturementioning

confidence: 99%

Multiomics Analysis and Systems Biology Integration Identifies the Roles of IL-9 in Keratinocyte Metabolic Reprogramming

Marathe

Dhamija

Kumar

et al. 2021

Journal of Investigative Dermatology

Self Cite

View full text Add to dashboard Cite

Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes

Abstract: High numbers of autologous human primary keratinocytes (HPKs) are required for patients with burns, wounds and for gene therapy of skin disorders.

Cited by 12 publications

References 43 publications

Extracellular Matrix protein gelatin provides higher expansion, reduces size heterogeneity, and maintains cell stiffness in a long-term culture of mesenchymal stem cells

Extracellular Matrix protein gelatin provides higher expansion, reduces size heterogeneity, and maintains cell stiffness in a long-term culture of mesenchymal stem cells

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

Multiomics Analysis and Systems Biology Integration Identifies the Roles of IL-9 in Keratinocyte Metabolic Reprogramming

Contact Info

Product

Resources

About