Hydrogen and carbon isotope fractionation during experimental production of bacterial methane

Balabane, May; Галимов, Э. М.; Hermann, M.; Lètolle, René

doi:10.1016/0146-6380(87)90033-7

Cited by 92 publications

(91 citation statements)

References 18 publications

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“…Methanosarcina barkeri α = 1.045 (Krzycki et al, 1987) and Methanobacterium formicicum α = 1.046 to 1.055 (Balabane et al, 1987). As previously noted, Methanosarcina and which is consistent with the molecular DNA analysis (Figures 2 and 3).…”

Section: Fractionation Factor For Determining Methanogenesis Pathwaysupporting

confidence: 78%

“…The current understanding regarding the fractionation of hydrogen isotopes during hydrogenotrophic methanogenesis is that the hydrogen in CH 4 produced by CO 2 reduction is solely derived from the ambient water and that the hydrogen isotopic fractionation between water and CH 4 is consistently between 160 ‰ and 180 ‰ (Schoell, 1980;Whiticar et al, 1986). However, other studies have reported that under certain conditions, the δ 2 H-CH 4 produced by CO 2 reduction is indistinguishable from that produced from cleavage of acetate, suggesting that not all hydrogen in CO 2 -derived CH 4 is necessarily obtained from water, and that acetoclastic methanogenesis acquired more than one atom of hydrogen from water (Balabane et al, 1987;Sugimoto and Wada, 1995). Furthermore, it has also been postulated that the relatively large hydrogen isotope fractionation found in closed system experiments may not be solely caused by high H 2 concentration.…”

Section: δ 2 H For Determination Of Methanogenesis Pathwaymentioning

confidence: 90%

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Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Susilawati

Golding

Baublys

et al. 2016

International Journal of Coal Geology

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Carbon and hydrogen isotope compositions of CH 4 generated via methanogenesis in cultures of South Sumatra Basin (SSB) coalbed methane (CBM) formation waters grown on coal, acetate and H 2 +CO 2 were investigated. CH 4 production and molecular analysis confirmed the presence of active microbial communities that are able to convert coal into CH 4 using both acetoclastic and hydrogenotrophic pathways. The representative bacterial sequences were dominated by Bacteroidetes, Firmicutes and Deltaproteobacteria, while Methanosaeta and Methanosarcina were the most prevalent archaeal methanogens present in the cultures.CH 4 produced in this study's culturing experiments has δ 13 C values in the range of -50 ‰ to -20 ‰, with most values falling outside the current understanding of the carbon isotopic boundaries for biogenic CH 4 (-110 ‰ to -30 ‰). However, the corresponding apparent carbon isotopic α factor (α c = 1.02±0.006), and isotopic effect (ε c = -20.1 ‰ ±15.3) showed that CH 4 in SSB cultures was predominantly produced by acetoclastic methanogenesis, which is consistent with the results of molecular DNA analysis. In addition, the calculated contribution of CO 2 reduction from the δ 13 C values of coaltreated cultures was overall < 50 %, further confirming the high contribution of the acetoclastic pathway to CH 4 production in the SSB cultures. The outcome of this experimental study also suggests that δ 2 H-CH 4 values may not provide a reliable basis for distinguishing methanogenic pathways, while apparent carbon isotopic fractionation factor (α c ) and isotope effect (ε c ) are considered more useful indicators of the methanogenic pathway.The high δ 13 C-CH 4 values (>-30 ‰) and the dominance of Methanosaeta over Methanosarcina indicate that methanogens within the SSB cultures were operating at low substrate concentrations. An unusually positive δ 13 C-CH 4 suggests a substrate depletion effect, which is thought to be related to a decrease in the relative abundance of key bacterial coal degraders with formation water inoculum storage time. Closer observation of δ 13 C-CH 4 values during the growth of cultures within a single experiment also showed a 13 C-enrichment trend over time. At log phase of growth, the CH 4 produced was 13 C-depleted when compared to the stationary phase that also indicates substrate depletion effects. Finally, the δ 13 C-CH 4 values encountered in this study (as high as -20 ‰) highlight the possible positive extension of δ 13 C-CH 4 values of acetoclastic methanogenesis from those currently reported in the literature for natural and experimental samples (as high as -30 ‰).Keywords: Biogenic gas, coal, culture experiment, isotopic composition INTRODUCTIONArchaeal methanogens are the only known microorganisms capable of forming CH 4 . These strictly anaerobic microbes are restricted to utilizing simple compounds that contain no more than two carbon atoms. As such, complex organic compounds in anoxic environments (e.g., marine sediments, rice paddies, subsurface shales and coalbeds) ...

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Section: Fractionation Factor For Determining Methanogenesis Pathwaysupporting

confidence: 78%

Section: δ 2 H For Determination Of Methanogenesis Pathwaymentioning

confidence: 90%

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Susilawati

Golding

Baublys

et al. 2016

International Journal of Coal Geology

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“…in anoxic layers of lake sediment and is carried out by methanogenic Archaea. Methanogenic Archaea could be the 13 C-depleted larval food source since their biomass often is strongly 13 C depleted (Fuchs et al 1979, Belyaev et al 1983, Balabane et al 1987. However, the only organisms known to be able to use methane as a carbon source and which are widespread in freshwater environments are aerobic methane-oxidising bacteria (MOB, Hanson & Hanson 1996).…”

Section: Abstract: Food Web · Chironomid Larvae · Symbionts · Methanmentioning

confidence: 99%

Possible sources of methane-derived carbon for chironomid larvae

Eller

Deines

Krüger³

2007

Aquat. Microb. Ecol.

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“…Dans des conditions plus exceptionnelles qui peuvent être celles des circuits très profonds, la production de CO 2 in situ est plus perturbatrice : -La fermentation méthanique dans des milieux très ré-ducteurs entraîne un fractionnement isotopique considéra-ble de l'ordre de 50 %0 [21] Toute la difficulté réside dans la détermination de l'activité initiale (A o )' Suivant les réactions déjà évoquées pour le carbone 13, l'essentiel du carbone minéral dissous résulte de l'activité en 14C du CO 2 du sol, fixée à 100 % de carbone moderne (avant les essais thermonucléaires) et de l'activité en 14C nulle des carbonates « morts » du sol et du réservoir. La valeur attendue est donc de 50 pcm mais l'expérience a montré que l'activité initiale était très supérieure.…”

Section: Utilisation Des Isotopes Des Esunclassified

Apports des techniques isotopiques à la connaissance des gisements d'eau minérale

Blavoux

1995

La Houille Blanche

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Hydrogen and carbon isotope fractionation during experimental production of bacterial methane

Cited by 92 publications

References 18 publications

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Possible sources of methane-derived carbon for chironomid larvae

Apports des techniques isotopiques à la connaissance des gisements d'eau minérale

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