Hydrogen and ethanol production from glycerol-containing wastes discharged after biodiesel manufacturing process

Ito, Takeshi; Nakashimada, Yutaka; Senba, Koichiro; Matsui, Tomoaki; Nishio, Naomichi

doi:10.1263/jbb.100.260

Cited by 464 publications

(310 citation statements)

References 29 publications

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“…performance of these engineered strains was superior to that reported for the conversion of glycerol to ethanol and hydrogen or ethanol and formate by other microorganisms (Ito et al, 2005;Jarvis et al, 1997;Sakai and Yagishita, 2007) and similar to that achieved with E. coli strains engineered to convert cellulosic sugars to ethanol (Underwood et al, 2002). In addition to producing ethanol, strain EF06 [pZSKLMgldA] produces formate, which implies an overall yield and rate of product synthesis almost twice of that reported for the production of ethanol from sugars.…”

Section: Engineered Strains For the Conversion Of Glycerol To Ethanolsupporting

confidence: 51%

Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli

Durnin

Clomburg

Yeates

et al. 2009

Biotech & Bioengineering

174

130

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Given its availability, low prices, and high degree of reduction, glycerol has become an ideal feedstock for the production of reduced compounds. The anaerobic fermentation of glycerol by Escherichia coli could be an excellent platform for this purpose but it requires expensive nutrients such as tryptone and yeast extract. In this work, microaerobic conditions were used as a means of eliminating the need for rich nutrients. Availability of low amounts of oxygen enabled redox balance while preserving the ability to synthesize reduced products. A fermentation balance analysis showed $95% recovery of carbon and reducing equivalents. The pathways involved in glycerol dissimilation were identified using different genetic and biochemical approaches. Respiratory (GlpK-GlpD/GlpABC) and fermentative (GldA-DhaKLM) routes mediated the conversion of glycerol to glycolytic intermediates. Although pyruvate formate-lyase (PFL) and pyruvate dehydrogenase contributed to the synthesis of acetyl-CoA from pyruvate, most of the carbon flux proceeded through PFL. The pathways mediating the synthesis of acetate and ethanol were required for the efficient utilization of glycerol. The microaerobic metabolism of glycerol was harnessed by engineering strains for the co-production of ethanol and hydrogen (EH05 [pZSKLMgldA]), and ethanol and formate (EF06 [pZSKLMgldA]). High ethanol yields were achieved by genetic manipulations that reduced the synthesis of byproducts succinate, acetate, and lactate. Co-production of hydrogen required the use of acidic pH while formate co-production was facilitated by inactivation of the enzyme formate-hydrogen lyase. High rates of product synthesis were realized by overexpressing glycerol dehydrogenase (GldA) and dihydroxyacetone kinase (DhaKLM). Engineered strains efficiently produced ethanol and hydrogen and ethanol and formate from glycerol in a minimal medium without rich supplements.

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Section: Engineered Strains For the Conversion Of Glycerol To Ethanolsupporting

confidence: 51%

Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli

Durnin

Clomburg

Yeates

et al. 2009

Biotech & Bioengineering

174

130

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“…Glycerol metabolism resulted in ethanol yield of 0.8 mol/mol feed. The yield of ethanol by fermenting glycerol could be enhanced to 0.85 mol/mol using immobilized bacteria [38]. Upscaling from small laboratory level reagent bottles to 3.6 L continuous culture stir tank reactors resulted in utilizing 1.5 % w/v glycerol for producing 0.75 mol ethanol/mole feed [39].…”

Section: Ethanolmentioning

confidence: 99%

Biorefinery for Glycerol Rich Biodiesel Industry Waste

2016

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The biodiesel industry has the potential to meet the fuel requirements in the future. A few inherent lacunae of this bioprocess are the effluent, which is 10 % of the actual product, and the fact that it is 85 % glycerol along with a few impurities. Biological treatments of wastes have been known as a dependable and economical direction of overseeing them and bring some value added products as well. A novel eco-biotechnological strategy employs metabolically diverse bacteria, which ensures higher reproducibility and economics. In this article, we have opined, which organisms and what bioproducts should be the focus, while exploiting glycerol as feed.

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“…9.24 x 10 9 Pa), which is very high and is not supposed to happen under biological conditions. In fact, glycerol is the maj or by-product of bio-diesel generation processes, and a further increase in the production of bio-diesel fuels would raise the problem of efficiently treating wastes containing glycerol (30). Therefore, apart from acetate, the possibility of using glycerol as the feedstock substrates in H2 production process also worthwhile to be investigated.…”

Section: Conversion Of Fermentation End-products Into H2: a Thermodynmentioning

confidence: 99%

Limitations of Bio-Hydrogen Production by Anaerobic Fermentation Process: An Overview

Cheng¹,

Cord‐Ruwisch²,

Ho³

2007

AIP Conference Proceedings

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Abstract. Turning organic wastes into hydrogen (H2) by using anaerobic fennentative technology is an ideal concept because this can accomplish both waste treatment and energy recovery objectives. However, H2 production using anaerobic fennentative approaches faces a fundamental limitation of poor H2 production yield (i.e. < 4 mol H2 per mol of hexose). This article gives an overview on the limitations of bio-H2 production using fennentative processes. Fundamental microbiology and thennodynamic ofthe processes are discussed.

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Hydrogen and ethanol production from glycerol-containing wastes discharged after biodiesel manufacturing process

Cited by 464 publications

References 29 publications

Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli

Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli

Biorefinery for Glycerol Rich Biodiesel Industry Waste

Limitations of Bio-Hydrogen Production by Anaerobic Fermentation Process: An Overview

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