Hydrogen as a New Class of Radioprotective Agent

Qian, Liren; Shen, Jianliang; Chuai, Yunhai; Cai, Jianming

doi:10.7150/ijbs.7220

Cited by 51 publications

(41 citation statements)

References 79 publications

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“…12,17) While there are several methods to deliver H 2 into the body including inhalation of H 2 gas, 18) injection of hydrogensaturated saline, 19) and drinking hydrogen water, 20) we utilized HW produced by electrolysis wherein H 2 was highly dissolved in PW passing through the hydraulic system of the generator, thereby producing characteristics such as neutral pH, extremely low ORP, and high DH.…”

Section: Discussionmentioning

confidence: 99%

“…11) Hydrogen molecule (H 2 ) has been widely used in medical applications as a safe and effective antioxidant and immunomodulator with minimal side effects. [12][13][14][15] Unlike other antioxidants, which are unable to target organelles, H 2 can penetrate biomembranes and diffuse into the cytosol, mitochondria and nucleus. 13) Moreover, H 2 has been reported to have •OHscavenging activity in culture medium.…”

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confidence: 99%

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Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Yoon

Sajo

Ignacio

et al. 2014

Biological & Pharmaceutical Bulletin

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Atopic dermatitis (AD) is a chronically relapsing, pruritic, eczematous skin disorder accompanying allergic inflammation. AD is triggered by oxidative stress and immune imbalance. In the present study, we investigated the effect of drinking hydrogen water (HW) on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in NC/Nga mice and found that HW ameliorated DNCB-induced AD-like clinical symptoms. In line with this, the level of reactive oxygen species in the HW group was significantly inhibited compared with that in the purified water (PW) group. In parallel, HW enhanced glutathione peroxidase activity in DNCBinduced AD as compared with the PW group. Accordingly, the levels of thymus and activation-regulated chemokine and cytokines were significantly decreased in the HW group compared with the PW group. Notably, the levels of Th 2 cytokine, interleukin-5 (IL-5), and proinflammatory cytokines such as tumor necrosis factor-α and IL-6 in HW-fed mice were significantly lower than in control and PW-fed mice. The total serum immunoglobulin E level was also markedly reduced in the HW group. The collective results indicate that HW suppresses DNCB-induced AD in NC/Nga mice via redox balance and immune modulation and could be a safe clinical fluid treatment for AD.Key words hydrogen water; atopic dermatitis; oxidative stress; immune modulation Atopic dermatitis (AD) is a chronically relapsing, pruritic, eczematous skin disorder accompanying allergic inflammation.1,2) AD is characterized by an impairment of the skinbarrier function, increased oxidative stress, and dysfunctional immune system. Intense infiltration of inflammatory cells release bioactive substance such as cytokines, chemokines, and reactive oxygen species (ROS).3) Of these AD's etiologic factors, inflammatory cytokines are known to regulate skin barrier, thereby aggravating the eczematous reaction in AD. 4)Most acute skin lesions in AD are exhibited by Th 2 inflammatory cytokines, whereas the chronic phase is characterized by Th 1 immune responses.5) The imbalance of cytokine network would play a critical role in the development of AD. 6,7)Together, oxidative stress such as increased ROS and lipid peroxidation is evident in any stage of AD. [8][9][10] An imbalance between ROS and antioxidants can lead to an elevated oxidative stress level. 11)Hydrogen molecule (H 2 ) has been widely used in medical applications as a safe and effective antioxidant and immunomodulator with minimal side effects.12-15) Unlike other antioxidants, which are unable to target organelles, H 2 can penetrate biomembranes and diffuse into the cytosol, mitochondria and nucleus. 13) Moreover, H 2 has been reported to have •OHscavenging activity in culture medium.14) Oral administration of hydrogen water (HW), inhalation of hydrogen gas, and injecting H 2 -dissolved saline is widely accepted to incorporate H 2 in the body. Recently, we reported that HW is effective in house mite-induced dermatitis via immunomodulation. 15) However, this study has a limitation owing to the lack of th...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Yoon

Sajo

Ignacio

et al. 2014

Biological & Pharmaceutical Bulletin

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“…Gut microbiome is rich in such bacteria. While these inorganic effector molecules are known to serve in various physiological and pathophysiological settings, [26, 345–347], their mechanisms of action are not fully understood at present.…”

Section: Carbon Monoxide and Hydrogenmentioning

confidence: 99%

Biological signaling by small inorganic molecules

Basudhar

Ridnour

Cheng

et al. 2016

Coordination Chemistry Reviews

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Small redox active molecules such as reactive nitrogen and oxygen species and hydrogen sulfide have emerged as important biological mediators that are involved in various physiological and pathophysiological processes. Advancement in understanding of cellular mechanisms that tightly regulate both generation and reactivity of these molecules is central to improved management of various disease states including cancer and cardiovascular dysfunction. Imbalance in the production of redox active molecules can lead to damage of critical cellular components such as cell membranes, proteins and DNA and thus may trigger the onset of disease. These small inorganic molecules react independently as well as in a concerted manner to mediate physiological responses. This review provides a general overview of the redox biology of these key molecules, their diverse chemistry relevant to physiological processes and their interrelated nature in cellular signaling.

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“…Thus, HRS has a strong reducing function and can neutralize free radicals within blood and cells [8]. HRS can easily enter cells, become involved in metabolism, and thereby promote cell detoxification, increase cell hydration, and enhance the body’s immune system [13, 14]. Li et al [15] investigated effects of HRS on prevention of renal injury induced by ischemia/reperfusion.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β Signaling Pathway in Rats

Chen¹,

Wang²,

Diao³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Cardiopulmonary bypass (CPB) is prone to inducing brain injury during open heart surgery. A hydrogen-rich solution (HRS) can prevent oxidation and apoptosis, and inhibit inflammation. This study investigated effects of HRS on brain injury induced by CPB and regulatory mechanisms of the PI3K/Akt/GSK3β signaling pathway. Methods: A rat CPB model and an in vitro cell hypoxia model were established. After HRS treatment, Rat behavior was measured using neurological deficit score; Evans blue (EB) was used to assess permeability of the blood-brain barrier (BBB); HE staining was used to observe pathological changes; Inflammatory factors and brain injury markers were detected by ELISA; the PI3K/Akt/GSK3β pathway-related proteins and apoptosis were assessed by western blot, immunohistochemistry and qRT –PCR analyses of brain tissue and neurons. Results: After CPB, brain tissue anatomy was disordered, and cell structure was abnormal. Brain tissue EB content increased. There was an increase in the number of apoptotic cells, an increase in expression of Bax and caspase-3, a decrease in expression of Bcl2, and increases in levels of Akt, GSK3β, P-Akt, and P-GSK3β in brain tissue. HRS treatment attenuated the inflammatory reaction ,brain tissue EB content was significantly reduced and significantly decreased expression levels of Bax, caspase-3, Akt, GSK3β, P-Akt, and P-GSK3β in the brain. After adding the PI3K signaling pathway inhibitor, LY294002, to rat cerebral microvascular endothelial cells (CMECs), HRS could reduce activated Akt expression and downstream regulatory gene phosphorylation of GSK3β expression, and inhibit CMEC apoptosis. Conclusion: The PI3K/Akt/GSK3β signaling pathway plays an important role in the mechanism of CPB-induced brain injury. HRS can reduce CPB-induced brain injury and inhibit CMEC apoptosis through the PI3K/Akt/GSK3β signaling pathway.

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Hydrogen as a New Class of Radioprotective Agent

Cited by 51 publications

References 79 publications

Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Positive Effects of Hydrogen Water on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in NC/Nga Mice

Biological signaling by small inorganic molecules

Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β Signaling Pathway in Rats

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