Hydrogen bonds energy distribution and information-theoretic analysis of blood serum from hamsters with experimental Graffi tumor

Abstract

The aim of the present study was to estimate the feasibility of blood serum hydrogen bonds energy distribution, and information-theoretic characteristics, as diagnostic markers for tumor disease in Graffi tumor bearing hamsters. Golden Syrian hamsters were randomly divided into two groups: a healthy control and an experimental one, challenged with Graffi tumor cells. The distributions of hydrogen bonds energies (-E) in the blood serum samples were investigated with a nonequilibrium process of droplets evaporation and sequential measurements of their wetting angle (Non-equilibrium Energy Spectrum (NES)). The measurements show significantly lower average hydrogen bond energy of the experimental samples than the controls. Shifts in NES toward depletion of (-E) at energy levels 0.0937, 0.1187, 0.1212, 0.1337, and 0.1387 eV, and an additional population at 0.0987, 0.1012, 0.1137, and 0.1237 eV energy levels were observed in the experimental samples compared to the controls. Such a pattern points to the possibility of utilizing the f(E) shifts at these energy levels as diagnostic markers of Graffi tumor in hamsters. Information-theoretic measures of Shannon entropy, Variation of information and Transformational information entropy were used to investigate possible differences between the experimental and control spectrum. Although they differ significantly in definitions, a comparison with the three information-theoretic measures did not reveal a statistically significant difference.

Effects of hydrogen-rich water on hamsters with experimental myeloid tumor

“…Our research shows that the anti-inflammatory effects of hydrogen-rich water are primarily associated with the peak at E=-0.1212 eV (39). The same peak was observed in the medical drugs for diabetes (53).…”

“…Furthermore, our previously published study also looked at hamsters with myeloid tumors, comparing them to healthy hamsters. statistically significant differences were observed in energy levels (-Е)=0.0937; 0.1187; 0.1212; 0.1337; 0.1387 eV (39). Additionally, there were observations regarding the effects of hydrogen-rich water (HRW) consumption on hydrogen bond energy distribution.…”

Physicochemical, Isotopic, Spectral, and Microbiological Analyses of Water From Glacier Mappa, Chilean Andes

“…The mathematical model of glacier water Mappa gives valuable information on the function of distribution of energies f(E) according to the values of (-E) of hydrogen bonds (Table 2 and Fig. 4) [38][39][40][41]. These distributions are connected with the restructuring of H2O molecules with the same energies.…”

Water Treated with Permanent Magnetic Field. Effects of Potassium Carbonate

“…E= -0.1387 eV; λ=8.95 µm; 1117 cm -1 is the local extremum for the restructuring of water molecules with the highest energies of hydrogen bonds for the configurations of water clusters from 16 to 24 water molecules (Neshev et al, 2022).…”