Hydrogen evolution catalyzed by viable and non-viable cells on biocathodes

Yates, Matthew D.; Siegert, Markus; Logan, Bruce E.

doi:10.1016/j.ijhydene.2014.08.015

Cited by 49 publications

(44 citation statements)

References 53 publications

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“…At the cost of high overpotentials and higher energy losses, hydrogen is formed under these conditions even at electrodes with low catalytic activity for H 2 evolution. This overpotential can be reduced when cell-derived enzymes or cofactors adsorb to the surface (Yates et al, 2014;Deutzmann et al, 2015). In our defined co-culture, hydrogen was formed already at − 400 mV, which enabled electrosynthesis with A. woodii that has been shown previously to be unable to carry out electrosynthesis (Nevin et al, 2011) due to efficient interspecies hydrogen transfer.…”

Section: Efficient Interspecies Hydrogen Transfer In Mixed Electrosynmentioning

confidence: 79%

“…Moreover, overpotentials of 4200 mV had to be applied repeatedly to achieve significant electron transfer rates (Villano et al, 2010;Aulenta et al, 2012). At these low potentials, the electrochemical formation of small reduced molecules as potential electron carriers such as H 2 , CO or formate at the cathode cannot be excluded (Villano et al, 2010;Yates et al, 2014;Deutzmann et al, 2015). To our knowledge, all methanogens and homoacetogens studied for their electrosynthetic properties are able to metabolize at least some of these small reduced molecules.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced microbial electrosynthesis by using defined co-cultures

2016

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Microbial uptake of free cathodic electrons presents a poorly understood aspect of microbial physiology. Uptake of cathodic electrons is particularly important in microbial electrosynthesis of sustainable fuel and chemical precursors using only CO 2 and electricity as carbon, electron and energy source. Typically, large overpotentials (200 to 400 mV) were reported to be required for cathodic electron uptake during electrosynthesis of, for example, methane and acetate, or low electrosynthesis rates were observed. To address these limitations and to explore conceptual alternatives, we studied defined co-cultures metabolizing cathodic electrons. The Fe(0)-corroding strain IS4 was used to catalyze the electron uptake reaction from the cathode forming molecular hydrogen as intermediate, and Methanococcus maripaludis and Acetobacterium woodii were used as model microorganisms for hydrogenotrophic synthesis of methane and acetate, respectively. The IS4-M. maripaludis co-cultures achieved electromethanogenesis rates of 0.1-0.14 μmol cm − 2 h − 1 at − 400 mV vs standard hydrogen electrode and 0.6-0.9 μmol cm − 2 h − 1 at − 500 mV. Co-cultures of strain IS4 and A. woodii formed acetate at rates of 0.21-0.23 μmol cm − 2 h − 1 at − 400 mV and 0.57-0.74 μmol cm − 2 h − 1 at − 500 mV. These data show that defined co-cultures coupling cathodic electron uptake with synthesis reactions via interspecies hydrogen transfer may lay the foundation for an engineering strategy for microbial electrosynthesis.

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Section: Efficient Interspecies Hydrogen Transfer In Mixed Electrosynmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Enhanced microbial electrosynthesis by using defined co-cultures

2016

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“…These images are consistent with other investigations that found inorganic and organic materials deposited on the electrode surface after being exposed to conditions meant to inhibit or remove biofilms (Jourdin et al 2016b;Yates, Siegert & Logan 2014). …”

Section: Inorganic and Organic Microbial Surface Modification Involvesupporting

confidence: 92%

“…These findings support previous research which hypothesises that an electroactive biofilm can activate its electrode surface, enhancing the catalysis of HER even after the biomass has been killed (Yates, Siegert & Logan 2014;Jourdin et al 2016b). …”

Section: Cathodic Biofilm Activates Electrode Surface and Achieves Efsupporting

confidence: 91%

“…In autotrophic microbial electrosynthesis, (Jourdin et al 2015a) showed that H 2 -mediated electron transfer drives the process of CO 2 reduction to acetate and suggested that biologically-driven Cu deposition on the electrode surface was responsible for the observed increase in hydrogen evolution. Another recent study by Yates et al 2014 suggested instead that enhanced hydrogen production resulted from cell debris, with living cells not required. Finally, another recent investigation found that cell-derived free enzymes such as hydrogenases can interact with cathodic surfaces and catalyse the formation of intermediates that are rapidly consumed by microbial cells (Deutzmann, Sahin & Spormann 2015 Biomass retention depends on many factors, such as cathode potential, electrode material, and type of inoculum used (Geppert et al 2016;Flexer et al 2016).…”

Section: Elucidation Of Autotrophic Sulfate-reducing Mechanisms In Bimentioning

confidence: 99%

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Bio-Electrochemical process for Metal and Sulfur Recovery from Acid Mine Drainage

Zamora¹,

Alonso²

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Water recycling and resource recovery from acid mine drainage (AMD) are increasingly being regarded as desirable practices with direct benefits for the environment and the operational and economic viability of the resources sector.This thesis proves the concept of a novel bioelectrochemical system (BES) for the direct electrode-driven resource recovery and practically permanent AMD treatment. The technology consists of a two-cell bioelectrochemical setup to enable the removal of sulfate from the ongoing reduction-oxidation sulfur cycle, thereby also reducing salinity, without external addition of chemicals.In particular, the goals of this thesis are: (i) to enrich a sulfate reducing bacterial community capable of directly utilising a carbon based cathode as electron donor for autotrophic sulfate reduction, or via bioelectrochemically-produced H 2 ; (ii) to elucidate the electron flux pathways of autotrophic sulfate reduction and microbial interactions in cathodic mixed cultures; (iii) to develop a high-rate sulfate reducing bioelectrochemical reactor based on high surface area electrode materials; (iv) to design and implement a combined chemical-free bioelectrochemical process that enables the sulfur, metal and water recovery from AMD.In order to elucidate whether cathodes can effectively release electrons and act as the only electron donor to support sulfate reduction process, the effect of cathode potential and inoculum source were evaluated using electrochemical tools, including the recording of chronoamperometry and cyclic/linear sweep voltammetry (Chapter 5). Electrochemical and off-gas analysis coupled to liquid phase sampling was carried out to determine the electron fluxes from the electrode to the final electron acceptor (sulfate) during autotrophic sulfate reduction (Chapter 6). Fluorescence in situ hybridization (FISH) and digital image analysis (DAIME) of the microbial communities in z-stack confirmed the microbial stratification. After obtaining a well-functioning biocathode for autotrophic sulfate reduction, its performance was experimentally optimised in terms of high-surface area electrode materials, like multi-wall carbon nanotubes on reticulated vitreous carbon (MWCNT-RVC) and carbon granules (CG) (Chapter 7). Finally, a novel BES was tested for AMD treatment , outcompeting methanogens and homoacetogens for the hydrogen without the need to add chemical inhibitors.The findings of this thesis show that inexpensive CG can achieve higher current-tosulfide efficiencies at lower power consumption than the nano-modified three-dimensional MWCNT-RVC. Sequencing analysis of the 16S rRNA gene on day 58 revealed that MWCNT-RVC retained the bacteria and archaea population from the inoculum, while CG electrode surface was able to select for bacteria over archaea.The feasibility to integrate a two-stage BES was proven with a lab-scale setup for AMD treatment. Using AMD, the BES operation enhanced the sulfate reduction rate ( The solid metal-sludge was 2 times less voluminous and 9 times more readily s...

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A Promising Strategy for Renewable Energy Recovery

Zhao

Zhang

2020

Bioelectrosynthesis

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Hydrogen evolution catalyzed by viable and non-viable cells on biocathodes

Cited by 49 publications

References 53 publications

Enhanced microbial electrosynthesis by using defined co-cultures

Enhanced microbial electrosynthesis by using defined co-cultures

Bio-Electrochemical process for Metal and Sulfur Recovery from Acid Mine Drainage

A Promising Strategy for Renewable Energy Recovery

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