Hydrogen peroxide and nitric oxide are involved in programmed cell death induced by cryopreservation in Dendrobium protocorm-like bodies

Xue, Jiang; Ren, Ruifen; Di, Wei; Jia, Mengxue; Li, Ze-di; Yan, Liying; Gao, Rongfu

doi:10.1007/s11240-019-01590-x

Cited by 28 publications

(20 citation statements)

References 57 publications

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“…The obtained data indicate that salt stress induces in the transgenic root cells the assembly of atypical elements of the cytoskeleton, which are morphologically identical to tubulin aggregates formed during experimentally induced ROS imbalance [2]. If we assume that the ectopic overexpression of the FeSOD gene in transgenic tomato plants leads to an increase in the tolerance to stress factors, the formation of atypical tubulin polymers under stress conditions accompanied by an increase in the ROS level is a manifestation of such a protective reaction [47]. It is assumed that the interaction of ROS and microtubules provides the perception of a stressful situation and triggers the corresponding response.…”

Section: Discussionmentioning

confidence: 74%

“…We expected a decrease in the ROS level in the root tissues of the FeSOD-transgenic line that was confirmed both for normal conditions without salt exposure and for salt treatment. The amount of ROS increases when plants are exposed to cold, salinity, drought, floods, and herbicide treatments [47], and, in general, during almost every type of biotic or abiotic stress [48]. Ion imbalance and hyperosmotic stress caused by the use of NaCl and Na 2 SO 4 result in further growth inhibition and various damage to macromolecules during the formation of ROS [49].…”

Section: Discussionmentioning

confidence: 99%

“…An injury of the cytoskeleton leading to a disturbance in the shape of cells ( Figure 1 and Figure 2 ) accompanies a change in the morphology of the meristematic cells in the present study ( Figure 4 and Figure 5 ). In some cases, these processes are accompanied and provided by autophagy [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

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Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

Bogoutdinova

Лазарева

Chaban

et al. 2020

Biology

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Various abiotic stresses cause the appearance of reactive oxygen species (ROS) in plant cells, which seriously damage the cellular structures. The engineering of transgenic plants with higher production of ROS-scavenging enzyme in plant cells could protect the integrity of such a fine intracellular structure as the cytoskeleton and each cellular compartment. We analyzed the morphological changes in root tip cells caused by the application of iso-osmotic NaCl and Na2SO4 solutions to tomato plants harboring an introduced superoxide dismutase gene. To study the roots of tomato plants cultivar Belyi Naliv (WT) and FeSOD-transgenic line, we examined the distribution of ROS and enzyme-linked immunosorbent detection of α-tubulin. In addition, longitudinal sections of the root apexes were compared. Transmission electronic microscopy of atypical cytoskeleton structures was also performed. The differences in the microtubules cortical network between WT and transgenic plants without salt stress were detected. The differences were found in the cortical network of microtubules between WT and transgenic plants in the absence of salt stress. While an ordered microtubule network was revealed in the root cells of WT tomato, no such degree of ordering was detected in transgenic line cells. The signs of microtubule disorganization in root cells of WT plants were manifested under the NaCl treatment. On the contrary, the cytoskeleton structural organization in the transgenic line cells was more ordered. Similar changes, including the cortical microtubules disorganization, possibly associated with the formation of atypical tubulin polymers as a response to salt stress caused by Na2SO4 treatment, were also observed. Changes in cell size, due to both vacuolization and impaired cell expansion in columella zone and cap initials, were responsible for the root tip tissue modification.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

Bogoutdinova

Лазарева

Chaban

et al. 2020

Biology

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“…As a signal mediator, NO is also involved in cold- and starvation-stress-induced programmed cell death in barley [ 92 ]. Jiang et al [ 93 ] showed that the level of NO began to accumulate and achieved the highest value at the loading stage of cryopreservation in Dendrobium .…”

Section: No and Drought Stressmentioning

confidence: 99%

Plant Nitric Oxide Signaling under Drought Stress

Lau

Hamdan

Pua

et al. 2021

Plants

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Water deficit caused by drought is a significant threat to crop growth and production. Nitric oxide (NO), a water- and lipid-soluble free radical, plays an important role in cytoprotection. Apart from a few studies supporting the role of NO in drought responses, little is known about this pivotal molecular amendment in the regulation of abiotic stress signaling. In this review, we highlight the knowledge gaps in NO roles under drought stress and the technical challenges underlying NO detection and measurements, and we provide recommendations regarding potential avenues for future investigation. The modulation of NO production to alleviate abiotic stress disturbances in higher plants highlights the potential of genetic manipulation to influence NO metabolism as a tool with which plant fitness can be improved under adverse growth conditions.

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“…Similarly, it was also found that the NO content changed signi cantly after cryopreservation of plants. In the cryopreservation of Dendrobium nobile protocorms by vitri cation, the accumulation of NO content in cells was accelerated by LN preservation (Jiang et al, 2019b).…”

Section: Introductionmentioning

confidence: 99%

Effects of Programmed Cell Death Induced by NO on Pollen Viability After Cryopreservation

Ren¹,

Zhou²,

Zhang³

et al. 2021

Preprint

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Low activity level of biomaterials after cryopreservation is a bottleneck problem that limited the application of this technology. At present, the mechanism of viability decline after cryopreservation is not fully understood. In this study, the effects of nitric oxide (NO) on programmed cell death (PCD) and its relationship with viability were investigated, using Paeonia lactiflora 'Fen Yu Nu' pollen with significantly decreased viability after cryopreservation. The results showed that: the activity of caspase-3-like and caspase-9-like protease and the apoptosis rate of pollen cells were significantly increased, the expression level of promoting PCD (pro-PCD) gene was up-regulated, while the expression level of inhibiting PCD (anti-PCD) gene was down-regulated after preserved in liquid nitrogen (LN); the NO content in pollen cells increased significantly after LN preserved. The results of correlation analysis showed that NO was significantly correlated with pollen viability and all indicators of PCD. The addition of NO carrier SNP after LN storage reduced pollen viability, increased endogenous NO content, decreased mitochondrial membrane potential level, activated caspase-3-like and caspase-9-like protease in pollen cells, and increased cell apoptosis rate, and the expression levels of pro-PCD genes PDCD2 and ATG8CL were significantly up-regulated, while the expression levels of anti-PCD genes DAD1, BI-1 and LSD1 were significantly down-regulated; and the addition of NO scavenger c-PTIO produced the opposite effect of SNP. It was suggested that NO induced the PCD during the cryopreservation of pollen, which was one of the reasons for the significant decrease of pollen viability after cryopreservation.

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Hydrogen peroxide and nitric oxide are involved in programmed cell death induced by cryopreservation in Dendrobium protocorm-like bodies

Cited by 28 publications

References 57 publications

Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

Plant Nitric Oxide Signaling under Drought Stress

Effects of Programmed Cell Death Induced by NO on Pollen Viability After Cryopreservation

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