Hydrogen peroxide generated by xanthine/xanthine oxidase system represses the proliferation of colorectal cancer cell line Caco-2

Sakuma, Satoru; Abe, Muneyuki; Kohda, Tetsuya; Fujimoto, Yohko

doi:10.3164/jcbn.14-34

Cited by 27 publications

(21 citation statements)

References 28 publications

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“…In addition, it is acknowledged that the expression of catalase in normal cells has been considered as mediator at the protein, polypeptide, delivering message, and bio-actively molecular levels. The cancerous cells applied in this study have minimal catalase active levels 36 . Swiftly actively growing cells, for instance cancerous cells, make aberrantly large amounts of hydrogen peroxide (H 2 O 2 ).…”

Section: Resultsmentioning

confidence: 99%

Heterogeneous Fenton Reaction Enabled Selective Colon Cancerous Cell Treatment

Lee

Chiu

et al. 2018

Sci Rep

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A selective colon cancer cell therapy was effectively achieved with catalase-mediated intra-cellular heterogeneous Fenton reactions triggered by cellular uptake of SnFe2O4 nanocrystals. The treatment was proven effective for eradicating colon cancer cells, whereas was benign to normal colon cells, thus effectively realizing the selective colon cancer cell therapeutics. Cancer cells possess much higher innate hydrogen peroxide (H2O2) but much lower catalase levels than normal cells. Catalase, an effective H2O2 scavenger, prevented attacks on cells by reactive oxygen species induced from H2O2. The above intrinsic difference between cancer and normal cells was utilized to achieve selective colon cancer cell eradication through endocytosing efficient heterogeneous Fenton catalysts to trigger the formation of highly reactive oxygen species from H2O2. In this paper, SnFe2O4 nanocrystals, a newly noted outstanding paramagnetic heterogeneous Fenton catalyst, have been verified an effective selective colon cancerous cell treatment reagent of satisfactory blood compatibility.

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Section: Resultsmentioning

confidence: 99%

Heterogeneous Fenton Reaction Enabled Selective Colon Cancerous Cell Treatment

Lee

Chiu

et al. 2018

Sci Rep

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“…For example, an intratumoral injection of adenoviral vector expressing E. coli PNP to accelerate inosine degradation shows safety and antitumor activity in the first-in-human clinical trial ( 76 ). Activation of XO suppresses disulfide bond formation of breast cancer resistance protein ( 77 ), and reactive oxygen species derived from XO further interrupt dimerization of breast cancer resistance protein ( 78 , 79 ).…”

Section: Purine Metabolism In Cancersmentioning

confidence: 99%

Potential Mechanisms Connecting Purine Metabolism and Cancer Therapy

et al. 2018

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Unrestricted cell proliferation is a hallmark of cancer. Purines are basic components of nucleotides in cell proliferation, thus impaired purine metabolism is associated with the progression of cancer. The de novo biosynthesis of purine depends on six enzymes to catalyze the conversion of phosphoribosylpyrophosphate to inosine 5′-monophosphate. These enzymes cluster around mitochondria and microtubules to form purinosome, which is a multi-enzyme complex involved in de novo purine biosynthesis and purine nucleotides requirement. In this review, we highlighted the purine metabolism and purinosome biology with emphasis on the therapeutic potential of manipulating of purine metabolism or purinosome in cancers. We also reviewed current advances in our understanding of mammalian target of rapamycin for regulating purinosome formation or purine metabolism in cancers and discussed the future prospects for targeting purinosome to treat cancers.

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“…ROS levels are indicators of the degree of oxidative stress (44). The biomarker 8-OHdG was first reported by Kasai et al (45) in 1984 and is now commonly used as a sensitive indicator of oxidative stress-induced DNA damage (46).…”

Section: Discussionmentioning

confidence: 99%

Effect of α2‑macroglobulin in the early stage of jaw osteoradionecrosis

Yin

Chen

et al. 2020

Int J Oncol

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Advanced osteoradionecrosis (ORN) is one of the most serious complications in patients with head and neck cancer, resulting in poor prognosis. Numerous studies have therefore focused on the pathogenesis and interventions of ORN early stage. The present study aimed to investigate whether α2-macroglobulin (α2M) could prevent early-stage jaw osteoradionecrosis caused by radiotherapy (RT). Following local injection of α2M, a single dose of 30 Gy was delivered to rats for pathological exploration. For 28 days, the irradiated mandible and soft tissues were examined for potential changes. Furthermore, primary human bone marrow mesenchymal stem cells pretreated with α2M followed by 8 Gy irradiation (IR) were also used. Tartrate-resistant acid phosphatase assay, terminal uridine deoxynucleotidyl nick end labeling assay and immunohistochemical staining were performed on irradiated mandibular bone, tongue or buccal mucosa tissues from rats. Cell proliferation was assessed by evaluating the cell morphology by microscopy and by using the cell counting kit-8. Fluorescence staining, flow cytometry and western blotting were conducted to detect the reactive oxygen species level, cell apoptosis and protein expression of superoxide dismutase 2 (SOD2), heme oxygenase-1 (HO-1) and phosphorylated Akt following irradiation. The results demonstrated that α2M attenuated physical inflammation, osteoclasts number and fat vacuole accumulation in mandibular bone marrow and bone marrow cell apoptosis following IR in vivo. Furthermore, α2M pretreatment suppressed the expression of 8-hydroxy-2'-deoxyguanosine in mandibular bone and tongue paraffin embedded sections, which is a marker of oxidative damage, and increased SOD2 expression in mucosa and tongue paraffin embedded sections. The present study demonstrated the efficient regulation of antioxidative enzymes, including SOD2 and heme oxygenase-1, and reduction in oxidative damage by α2M. In addition, in vitro results confirmed that α2M may protect cells from apoptosis and suppress reactive oxygen species accumulation. Overall, the present study demonstrated that α2M treatment may exert some radioprotective effects in early-stage ORN via antioxidant mechanisms, and may therefore be considered as a potential alternative molecule in clinical prophylactic treatments.

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Hydrogen peroxide generated by xanthine/xanthine oxidase system represses the proliferation of colorectal cancer cell line Caco-2

Cited by 27 publications

References 28 publications

Heterogeneous Fenton Reaction Enabled Selective Colon Cancerous Cell Treatment

Heterogeneous Fenton Reaction Enabled Selective Colon Cancerous Cell Treatment

Potential Mechanisms Connecting Purine Metabolism and Cancer Therapy

Effect of α2‑macroglobulin in the early stage of jaw osteoradionecrosis

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