Hydrogen peroxide–induced DNA damage and DNA repair in lymphocytes from malnourished children

González, Cristina; Nájera, Oralia; Cortés, Edith; Toledo, G.; López, Laura Svidler; Betancourt, Miguel; Ortiz, Rocı́o

doi:10.1002/em.10038

Cited by 31 publications

(26 citation statements)

References 46 publications

(49 reference statements)

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“…Furthermore, increased DNA damage and decreased DNA repair capacity have been found in children suffering from malnutrition, a common problem in developing countries. 21 When the effect of dental X-ray exposure in children was investigated, no statistically significant differences (p . 0.05) were found between micronucleated oral mucosa cells before and after radiation exposure.…”

Section: Resultsmentioning

confidence: 99%

Cytogenetic biomonitoring in oral mucosa cells following dental X-ray

Ribeiro¹

2012

Dentomaxillofacial Radiology

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Objectives: In the past decades, X-rays have been used widely for diagnosis in dentistry. However, it is well known that ionizing radiation causes damage (including single-and double-strand breaks) to deoxyribonucleic acid (DNA) and DNA-protein crosslinks, and induces cellular death. Therefore, outlining the cytogenetic effects induced by X-ray is necessary to identify the degree of cancer risk and minimize potential risks to patients and clinicians. To date, a variety of assays have been proposed in cytogenetic biomonitoring studies, including those that assess metaphase chromosomal aberrations and sister chromatid exchanges, and micronucleus and single-cell gel (comet) assay. Methods: Cytogenetic biomonitoring studies focusing on oral mucosa cells in individuals exposed to dental X-ray were reviewed. Results: Dental X-ray can induce DNA damage and cytotoxicity in oral mucosa cells. Conclusion: These results will contribute to a better understanding of X-ray-induced effects upon the cellular system in individuals continually exposed to known genotoxic/cytotoxic agents.

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Section: Resultsmentioning

confidence: 99%

Cytogenetic biomonitoring in oral mucosa cells following dental X-ray

Ribeiro¹

2012

Dentomaxillofacial Radiology

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“…The lack of nutrients may lead to the impaired synthesis of proteins associated with DNA integrity and/or to a depressed ability of cells from malnourished organisms to repair normal levels of DNA damage [González et al, 2002]. Fenech [2002] has reviewed the necessary role played by vitamins and minerals in various DNA maintenance reactions and proposed the relevance of optimal micronutrient intake for the prevention of genomic instability.…”

Section: Discussionmentioning

confidence: 99%

Spontaneous and mitomycin C‐induced micronuclei in peripheral blood reticulocytes from severely malnourished rats

Ortiz

Medina

Rodrı́guez

et al. 2004

Environ and Mol Mutagen

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Severe malnutrition caused by deficiencies in protein, calorie, and micronutrient intake is widely distributed throughout the world and is a particular problem in developing countries. Animal models have been useful for studying the effects of malnutrition under different experimental conditions. In this study, we have evaluated the effect of malnutrition on the frequency of spontaneous and mitomycin C (MMC)-induced micronuclei in the peripheral blood of rats measured using a flow cytometric analysis technique. Neonatal rats were experimentally malnourished during lactation and assayed at weaning (21 days of age). The malnourished rats weighed 49.2% less than well-nourished controls and had lower concentrations of serum protein, triglycerides, and cholesterol. In rats not treated with MMC, the frequency of micronucleated reticulocytes (MN-RETs) was 1.6 times greater in malnourished rats than in well-nourished rats (0.48% +/- 0.16% vs. 0.31% +/- 0.09%). The mean MN-RET frequency measured 32 hr after treatment with single i.p. doses of 0.5, 0.75, or 1.0 mg/kg of MMC was 0.60 +/- 0.10 vs. 0.84 +/- 0.14, 1.21 +/- 0.52 vs. 2.36 +/- 0.47, and 2.50 +/- 0.06 vs. 4.64 +/- 1.14 for well-nourished vs. malnourished rats, respectively. Statistical comparisons indicate significant differences between the two groups of rats at all doses tested. Malnourishment and MMC treatment had no significant effects on the frequencies of RETs or micronucleated normochromatic erythrocytes. The data indicate that protein-calorie malnutrition during lactation is associated with increased frequencies of MN-RETs, which are indicative of chromosome damage. These findings suggest that malnutrition could result in greater susceptibility to environmental damage.

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“…Observational studies of Mexican children suffering from Protein Energy Malnutrition (PEM) (6 with marasmus and one with kwashiorkor) suggest that severe malnutrition can impair DNA repair [26]. However, in the same study, bacterial infection appeared to be without effect on the capacity for DNA repair [26].…”

Section: Nutritional Modulation Of Dna Repairmentioning

confidence: 92%

Nutrition and DNA Repair - Potential Molecular Mechanisms of Action

Mathers¹,

Coxhead²,

Tyson

2007

CCDT

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At its most fundamental, cancer is a genetic disease in the sense that the primary events in tumorigenesis involve damage to the genome. The genome is subject to damage continuously from both exogenous agents and endogenous processes but this becomes functionally important only if the damage is not detected and resolved in a timely and effective manner. In mammals there are 5 DNA repair pathways, encoded by approximately 150 genes, which appear to have arisen early in evolution and which are highly conserved. Given the substantial epidemiological and experimental evidence that variation in dietary intake accounts for a significant proportion of the variance in cancer prevalence, an a priori case can be made that dietary factors may influence the effectiveness of DNA repair. A review of the literature has identified 4 observational and 8 intervention studies in human subjects where DNA repair (or a component thereof) has been measured in relation to nutrition. This rather limited evidence base precludes drawing definitive conclusions, but the fact that there were significant effects of dietary supplements in 5 out of the 8 intervention studies suggests that food components and/or nutritional status may influence DNA repair. This review considers possible molecular mechanisms through which such factors could modulate repair.

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Hydrogen peroxide–induced DNA damage and DNA repair in lymphocytes from malnourished children

Cited by 31 publications

References 46 publications

Cytogenetic biomonitoring in oral mucosa cells following dental X-ray

Cytogenetic biomonitoring in oral mucosa cells following dental X-ray

Spontaneous and mitomycin C‐induced micronuclei in peripheral blood reticulocytes from severely malnourished rats

Nutrition and DNA Repair - Potential Molecular Mechanisms of Action

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