Hydrogen Peroxide is Involved in Hamster Sperm Capacitation in Vitro1

Bize, Isabel; Santander, Gabriel; Cabello, P.; Driscoll, David F.; Sharpe, C. A.

doi:10.1095/biolreprod44.3.398

Cited by 161 publications

(66 citation statements)

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“…9). As the latter specifically scavenges hydrogen peroxide, these data support the notion that this oxidant is a physiological mediator of sperm function, driving both the tyrosine phosphorylation events associated with sperm capacitation (Bize et al 1991, Griveau et al 1994, Rivlin et al 2004, O'Flaherty et al 2006a and facilitating the acrosome reaction (Hsu et al 1999, O'Flaherty et al 2005. It is interesting to note that overall, phenylalanine is a much more powerful stimulus for the acrosome reaction than progesterone.…”

Section: Discussionsupporting

confidence: 68%

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Houston¹,

Curry²,

Aitken³

2015

Reproduction

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Reactive oxygen species (ROS) are known to play an important role in the regulation of human sperm function. In this study, we demonstrate for the first time that human spermatozoa possess interleukin-induced gene 1 (IL4I1), an L-amino acid oxidase (LAAO) which is capable of generating ROS on exposure to aromatic amino acids in the presence of oxygen. The preferred substrates were found to be phenylalanine and tryptophan while the enzyme was located in the acrosomal region and midpiece of these cells. In contrast to equine and bovine spermatozoa, enzyme activity was lost as soon as the spermatozoa became non-viable. On a cell-to-cell basis human spermatozoa were also shown to generate lower levels of hydrogen peroxide than their equine counterparts on exposure to phenylalanine. Stimulation of LAAO activity resulted in the induction of several hallmarks of capacitation including tyrosine phosphorylation of the sperm flagellum and concomitant activation of phospho-SRC expression. In addition, stimulation of LAAO resulted in an increase in the levels of acrosomal exocytosis in both the presence and absence of progesterone stimulation, via mechanisms that could be significantly reversed by the presence of catalase. As is often the case with free radical-mediated phenomena, prolonged exposure of human spermatozoa to phenylalanine resulted in the stimulation of apoptosis as indicated by significant increases in mitochondrial superoxide generation and the activation of intracellular caspases. These results confirm the existence of an LAAO in human spermatozoa with a potential role in driving the redox regulation of sperm capacitation and acrosomal exocytosis.

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Section: Discussionsupporting

confidence: 68%

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Houston¹,

Curry²,

Aitken³

2015

Reproduction

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“…Under these conditions, ROS play key roles in maintaining sperm kinematic and fusigenic functions. A specific ROS, H 2 O 2 , is involved in the hyperactivated movement necessary for spermatozoa to traverse the female genital tract and cumulus cells (Bize et al, 1991). ROS initiate lipid peroxidation, which increases sperm fusigenic func-tions, and which is necessary for binding and traversing the zona pellucida (Aitken et al, 1989).…”

Section: Oxidative Stress Contributes To Abnormal Chromatin Structurementioning

confidence: 99%

Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Evenson

Larson

Jost

2002

Journal of Andrology

909

768

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The Sperm Chromatin Structure Assay (SCSA) was first described in the December 5, 1980 issue of Science (Evenson et al, 1980a). The data described in that article showed a significant difference between proven fertile and subfertility or infertility in men and bulls and the susceptibility to denaturation of their sperm nuclear DNA. A subsequent study (Evenson et al, 1984) described SCSA data obtained from men with testicular cancer. The data were heterogeneous, and begged the question, what was SCSA measuring? Great effort has been spent over the past 15 years on animal model systems, mostly related to dose-response toxicology experiments and large animal fertility trials. The results from these studies showed that SCSA was highly dose-responsive to toxicants, highly repeatable, and provided meaningful biological information on sperm nuclear DNA defects. The extensive data from nonhumans (Evenson et al, 1985(Evenson et al, , 1989a(Evenson et al, ,b, 1993a(Evenson et al, ,b,c, 1994Ballachey et al, 1987Ballachey et al, , 1988Evenson and Jost, 1993; Sailer et al, 1995a,b;Evenson, 1999a), and human toxicology and fertility studies (Evenson et al, 1978(Evenson et al, , 1980a(Evenson et al, ,b, 1984(Evenson et al, , 1991Evenson and Melamed, 1983;Evenson, 1999b;Larson et al, 1999Larson et al, , 2000Larson et al, , 2001 provide This work was supported in part by grant R827019 from the US Environmental Protection Agency, grants EPS-9720642 and OSR-9452894 from the National Science Foundation, and by SCSA Diagnostics, Inc. This is South Dakota Agricultural Experiment Station publication 3273 of the journal series.Correspondence to: Donald P. Evenson, Olson Biochemistry Laboratories, ASC 136, South Dakota State University, Brookings, SD 57006 (e-mail: donald evenson@sdstate.edu).Received for publication September 20, 2001; accepted for publication September 20, 2001. compelling evidence that SCSA will be useful in clinical human semen analysis (Figure 1).After reviewing current infertility center sites on the World Wide Web and approximately a dozen lay-oriented books on infertility and assisted reproductive technology (ART), it was amazing that not a single reference contained any information about the negative influence of fragmented sperm nuclear DNA on successful pregnancy outcome. Furthermore, few physicians are aware of scientific literature on this subject. Currently, if a spermatozoon has some motility as seen under a light microscope, and reasonable morphology, then many ART clinics assume that the sperm DNA must be fine. However, a number of cases have been documented in which couples have had multiple failed attempts at intracytoplasmic sperm injection (ICSI), even using donor eggs, and we found that the fragmentation of sperm DNA was above our threshold for less than 1% probability of achieving a successful pregnancy. Spermatozoa with defective DNA can fertilize an oocyte, produce high-quality early stage embryos, and then, in relationship to the extent of DNA damage, fail in producing a successful term ...

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“…Because the sperm-oocyte fusion in this test is taken as representative for AR status, ROS seemed to inhibit AR. However, the study of Bize et al [6] suggested that the presence of H,O, (but not superoxide anions) is necessary for AR in hamster sperm, and a recent study of de Lamirande and Gagnon [ 1 11 shows a possible role of ROS (particularly the superoxide anion) in triggering hyperactivation and capacitation of human spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cytokines on Sperm Motility and Ionophore—Stimulated Acrosome Reaction

Fedder

Ellermann-Eriksen

1995

Archives of Andrology

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The effects of soluble products from leukocytes (WBC) on sperm motility and calcium ionophorestimulated acrosome reaction (AR) were examined. Supernatants of mononuclear WBC, isolated from peripheral blood and stimulated by the lectins Phyfolacca americana (pokeweed mitogen) or concanavalin A, caused a weak but significant inhibition of progressive sperm motility. The recombinant cytokine interferon-y (IFN-y) in high concentrations inhibited motility of sperm from 5 of 8 donors tested. The recombinant cytokines tumor necrosis factor-a (TNF-a) and interleukin-8 (IL-8) did not show any effect on sperm motility. Reactive oxygen species (ROS) produced by stimulation of polymorphonuclear WBC with a phorbol ester (PMA) tended to inhibit sperm motility. Neither supernatants from lectin-stimulated mononuclear WBC, IFN-y, TNF-a, IL-8, nor ROS showed any significant effect on the ionophore-challenged AR. acrosome reaction, sperm motility, cytokines, reactive oxygen species, lectins, human KeywordsHigh concentration of leukocytes in semen may be a causative mediator of otherwise unexplained infertility [ 151. Cytokines produced by these cells could be responsible for the effect. Thus, in a few studies an inhibiting role has been found of the cytokines interferon-? (IFN-y) and tumor necrosis factor-a (TNF-a) on motility of human sperm [ 14, 181 and on penetration rate in the hamster oocyte test [17],The cytokine interleukin-8 (IL-8) stimulates chemotaxis of human polymorphonuclear granulo-

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Hydrogen Peroxide is Involved in Hamster Sperm Capacitation in Vitro1

Cited by 161 publications

References 0 publications

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Effect of Cytokines on Sperm Motility and Ionophore—Stimulated Acrosome Reaction

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