Hydrogen Peroxide is Involved in Salicylic Acid-Induced Adventitious Rooting in Cucumber Under Cadmium Stress

Gong, Wenting; Niu, Lijuan; Wang, Chunlei; Wei, Lijuan; Pan, Ying; Liao, Weibiao

doi:10.1007/s12374-021-09332-3

Cited by 13 publications

(10 citation statements)

References 42 publications

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“…The central aspect of expanded plant cell growth is the hydrolysis of the polysaccharides that make up the cell wall by a series of hydrolytic enzymes (cell wall relaxing factors), which results in cell wall relaxation. Our previous study confirmed that salicylic acid (SA) treatment increased pectin content and reduced the degree of pectin methylation under C d stress, thereby promoting AR formation in cucumber [ 38 ]. Cellulose is the main load-bearing component of the primary cell wall.…”

Section: Discussionmentioning

confidence: 57%

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Hua²,

Hou

et al. 2023

BMC Plant Biol

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Background Methane (CH4) and brassinosteroids (BRs) are important signaling molecules involved in a variety of biological processes in plants. Results Here, marigold (Tagetes erecta L. ‘Marvel’) was used to investigate the role and relationship between CH4 and BRs during adventitious root (AR) formation. The results showed a dose-dependent effect of CH4 and BRs on rooting, with the greatest biological effects of methane-rich water (MRW, CH4 donor) and 2,4-epibrassinolide (EBL) at 20% and 1 μmol L− 1, respectively. The positive effect of MRW on AR formation was blocked by brassinoazole (Brz, a synthetic inhibitor of EBL), indicating that BRs might be involved in MRW-regulated AR formation. MRW promoted EBL accumulation during rooting by up-regulating the content of campestanol (CN), cathasterone (CT), and castasterone (CS) and the activity of Steroid 5α-reductase (DET2), 22α-hydroxylase (DWF4), and BR-6-oxidase (BR6ox), indicating that CH4 could induce endogenous brassinolide (BR) production during rooting. Further results showed that MRW and EBL significantly down-regulated the content of cellulose, hemicellulose and lignin during rooting and significantly up-regulated the hydrolase activity, i.e. cmcase, xylanase and laccase. In addition, MRW and EBL also significantly promoted the activity of two major cell wall relaxing factors, xyloglucan endotransglucosylase/hydrolase (XTH) and peroxidase, which in turn promoted AR formation. While, Brz inhibited the role of MRW on these substances. Conclusions BR might be involved in CH4-promoted AR formation by increasing cell wall relaxation.

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Section: Discussionmentioning

confidence: 57%

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Hua²,

Hou

et al. 2023

BMC Plant Biol

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“…These results showed that Cd stress could significantly inhibit the process of adventitious root in cucumber explants. Previous studies found that Cd stress inhibited the adventitious root formation in plants ( Li et al., 2019 ; Gong et al., 2022 ). However, suitable concentration of NO treatment significantly promoted adventitious root formation under Cd stress ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 90%

Nitric oxide promotes adventitious root formation in cucumber under cadmium stress through improving antioxidant system, regulating glycolysis pathway and polyamine homeostasis

Niu

Tang²,

Zhu³

et al. 2023

Front. Plant Sci.

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Cadmium (Cd) as a potentially toxic heavy metal that not only pollutes the environment but also interferes with plant growth. Nitric oxide (NO) regulates plant growth and development as well as abiotic stress response. However, the mechanism underpinning NO-induced adventitious root development under Cd stress remains unclear. In this study, cucumber (Cucumis sativus ‘Xinchun No. 4’) was used as the experimental material to investigate the effect of NO on the development of adventitious roots in cucumber under Cd stress. Our results revealed that, as compared to Cd stress, 10 μM SNP (a NO donor) could considerably increase the number and length of adventitious roots by 127.9% and 289.3%, respectively. Simultaneously, exogenous SNP significantly increased the level of endogenous NO in cucumber explants under Cd stress. Our results revealed that supplementation of Cd with SNP significantly increased endogenous NO content by 65.6% compared with Cd treatment at 48 h. Furthermore, our study indicated that SNP treatment could improve the antioxidant capacity of cucumber explants under Cd stress by up-regulating the gene expression level of antioxidant enzymes, as well as reducing the levels of malondialdehyde (MDA), hydrogen peroxide (H2O2) and superoxide anion (O2.-) to alleviate oxidative damage and membrane lipid peroxidation. Application of NO resulted in a decrease of the O2.-, MDA, and H2O2 level by 39.6%, 31.4% and 60.8% as compared to Cd-alone treatment, respectively. Besides that, SNP treatment significantly increased the expression level of related genes involved in glycolysis processes and polyamine homeostasis. However, application of NO scavenger 2-(4-carboxy -2-phenyl)-4, 4, 5, 5-tetramethy limidazoline -1-oxyl -3-oxide (cPTIO) and the inhibitor tungstate significantly reversed the positive role of NO in promoting the adventitious root formation under Cd stress. These results suggest that exogenous NO can increase the level of endogenous NO, improve antioxidation ability, promote glycolysis pathway and polyamine homeostasis to enhance the occurrence of adventitious roots in cucumber under Cd stress. In summary, NO can effectively alleviate the damage of Cd stress and significantly promote the development of adventitious root of cucumber under Cd stress.

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“…During the next phase (the induction or late induction phase), these cells become committed to forming the first root initials [ 9 , 16 ]. Right after, a series of cell divisions occur, where the organized mass of cells is visible, forming the first root meristem, which will gradually develop into root primordium (root initiation) [ 4 , 9 , 19 , 20 , 21 ]. The latter will continue to grow in order to connect to the vascular system of the cutting [ 7 ] while at the same time, if the conditions are favorable, it will emerge from the stem as a functional, developing root (expression phase or root emergence) [ 4 , 18 ].…”

Section: Insights Into Adventitious Root Formation and Factors Affect...mentioning

confidence: 99%

“…It also has a close relationship with some plant growth regulators, indirectly affecting plant physiological and molecular responses. It has been found to participate in the signaling pathways of cytokinins, jasmonic acid, indole-3-acetic acid, and ethylene [ 1 , 20 ]. At the same time, H 2 O 2 is involved in the following processes: The regulation of plant defense arsenal against biotic and abiotic stress factors; Seed germination; The photosynthetic machinery; Cell viability and stomatal responses; The regulation of several proteins and the biosynthesis of amino acids; Cellular differentiation; Plant morphogenesis, and others [ 1 , 3 , 20 , 55 ].…”

Section: The Role and Function Of Hydrogen Peroxide In Root Formationmentioning

confidence: 99%

Adventitious Root Formation in Plants: The Implication of Hydrogen Peroxide and Nitric Oxide

Roussos

2023

Antioxidants

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Adventitious root formation is defined as the formation of new roots on above-ground plant parts and is considered crucial for the survival of a plant under harsh environmental conditions (i.e., flooding, salt stress, and other abiotic stresses) as well as in the nursery industry. Clonal propagation is based on the ability of a plant part to grow and generate a completely new plant, genetically identical to the mother plant, where the plant part came from. Nurseries exploit this potential by multiplying millions of new plants. Most nurseries use cuttings to achieve that, through the induction of adventitious root formation. Many factors have been implicated in the capacity of a cutting to root, with the major role being played by auxins. During the last few decades, intense interest has emerged in the role of other potential rooting co-factors, such as carbohydrates, phenolics, polyamines, and other plant growth regulators, as well as signal molecules, such as reactive oxygen and nitrogen species. Among the latter, hydrogen peroxide and nitric oxide have been found to play significant roles in adventitious root formation. Their production, action, and general implication in rhizogenesis are discussed in this review, in terms of interaction with other molecules and signaling.

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Hydrogen Peroxide is Involved in Salicylic Acid-Induced Adventitious Rooting in Cucumber Under Cadmium Stress

Cited by 13 publications

References 42 publications

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Nitric oxide promotes adventitious root formation in cucumber under cadmium stress through improving antioxidant system, regulating glycolysis pathway and polyamine homeostasis

Adventitious Root Formation in Plants: The Implication of Hydrogen Peroxide and Nitric Oxide

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