Hydrogen Peroxide Response in Leaves of Poplar (Populus simonii × Populus nigra) Revealed from Physiological and Proteomic Analyses

Yu, Juanjuan; Jin, Xin; Sun, Xiaomei; Gao, Tianxiang; Chen, Xiaomei; She, Yi‐Min; Jiang, Tingbo; Chen, Sixue; Dai, Shaojun

doi:10.3390/ijms18102085

“…Consistent with that, a 14-3-3 protein and a nucleoside diphosphate kinase (NDPK) were upregulated in both plant groups. These proteins have been implicated in abiotic stress response, e.g., in hydroperoxide-responsive signaling pathways in leaves [44,45]. This might imply that this pathway is active in salt-stressed C. glauca plants independent of whether the plants are N-deprived or not and may explain the reduction of catalase levels which would be required for the increased production of hydrogen peroxide for signaling.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Nodulated and Non-Nodulated Casuarina glauca Sieb. ex Spreng. Grown under Salinity Conditions Using Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)

Graça

¹

,

Mendes

²

,

Marques

³

et al. 2019

IJMS

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Casuarina glauca displays high levels of salt tolerance, but very little is known about how this tree adapts to saline conditions. To understand the molecular basis of C. glauca response to salt stress, we have analyzed the proteome from branchlets of plants nodulated by nitrogen-fixing Frankia Thr bacteria (NOD+) and non-nodulated plants supplied with KNO3 (KNO3+), exposed to 0, 200, 400, and 600 mM NaCl. Proteins were identified by Short Gel, Long Gradient Liquid Chromatography coupled to Tandem Mass Spectrometry and quantified by Sequential Window Acquisition of All Theoretical Mass Spectra -Mass Spectrometry. 600 proteins were identified and 357 quantified. Differentially Expressed Proteins (DEPs) were multifunctional and mainly involved in Carbohydrate Metabolism, Cellular Processes, and Environmental Information Processing. The number of DEPs increased gradually with stress severity: (i) from 7 (200 mM NaCl) to 40 (600 mM NaCl) in KNO3+; and (ii) from 6 (200 mM NaCl) to 23 (600 mM NaCl) in NOD+. Protein–protein interaction analysis identified different interacting proteins involved in general metabolic pathways as well as in the biosynthesis of secondary metabolites with different response networks related to salt stress. Salt tolerance in C. glauca is related to a moderate impact on the photosynthetic machinery (one of the first and most important stress targets) as well as to an enhancement of the antioxidant status that maintains cellular homeostasis.

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“…Glycine betaine content was determined using Reinecke salt according to a method from Yu et al [77]. Briefly, fresh callus was ground to powder in liquid nitrogen, and then incubated in 6 mL 0.375%…”

Section: Measurements Of Mda Proline Total Soluble Sugar and Glycimentioning

confidence: 99%

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

Zhang

¹

,

Zhang

²

,

Yu

³

et al. 2019

Preprint

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Background: Salinity has obvious effects on plant growth and crop productivity. The salinity-responsive mechanisms have been well-studied in differentiated organs (e.g., leaves, roots and stems), but not in unorganized cells such as callus. High-throughput quantitative proteomics approaches have been used to investigate callus development, somatic embryogenesis, organogenesis, and stress response in numbers of plant species. However, they have not been applied to callus from monocotyledonous halophyte alkaligrass (Puccinellia tenuifora). Results: The alkaligrass callus growth, viability and membrane integrity were perturbed by 50 mM and 150 mM NaCl treatments. Callus cells accumulated the proline, soluble sugar and glycine betaine for the maintenance of osmotic homeostasis. Importantly, the activities of ROS scavenging enzymes (e.g., SOD, APX, POD, GPX, MDHAR and GR) and antioxidants (e.g., ASA, DHA and GSH) were induced by salinity. The abundance patterns of 55 salt-responsive proteins indicate that salt signal transduction, cytoskeleton, ROS scavenging, energy supply, gene expression, protein synthesis and processing, as well as other basic metabolic processes were altered in callus to cope with the stress. Conclusions: The undifferentiated callus exhibited unique salinity-responsive mechanisms for ROS scavenging and energy supply. Activation of the POD pathway and AsA-GSH cycle was universal in callus and differentiated organs, but salinity-induced SOD pathway and salinity-reduced CAT pathway in callus were different from those in leaves and roots. To cope with salinity, callus mainly relied on glycolysis, but not the TCA cycle, for energy supply. Keywords: Salinity response, ROS scavenging, Energy supply, Osmotic homeostasis, Callus, Halophyte alkaligrass, Proteomics

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“…The proline content was calculated with concentration (C), volume of total extraction solution (V), and Glycine betaine content was determined using Reinecke salt according to a method from Yu et al [77]. Briefly, fresh callus was ground to powder in liquid nitrogen, and then incubated in 6 mL 0.375% (w/v) reinecke salt solution for 24 h. The homogenate was centrifuged at 10,000× g at 20 °C for 15 min.…”

Section: Measurements Of Mda Proline Total Soluble Sugar and Glycimentioning

confidence: 99%

“…H 2 O 2 content was determined spectrophotometrically after reaction with potassium iodide [78]. The O 2 •− generation rate was detected using a hydroxylamine oxidization method [77].…”

Section: Ros Measurement and Enzyme Activity Assaymentioning

confidence: 99%

“…One unit of SOD activity was defined as the amount of enzyme that inhibited 50% of NBT photoreduction, and inhibition of the photochemical reduction of nitro blue tetrazolium (NBT) was detected at 560 nm. CAT activity was measured by detecting H 2 O 2 consumption at 240 nm, and POD activity was determined by a guaiacol method at 470 nm [77]. APX activity and GPX activity were measured by monitoring the absorbance changes at 290 nm and 340 nm, according to the oxidized ascorbate and NADPH, respectively.…”

mentioning

confidence: 99%

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NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

Zhang

¹

,

Zhang

²

,

Yu

³

et al. 2019

Preprint

Self Cite

0

View full text Add to dashboard Cite

Background: Salinity has obvious effects on plant growth and crop productivity. The salinity-responsive mechanisms have been well-studied in differentiated organs (e.g., leaves, roots and stems), but not in unorganized cells such as callus. High-throughput quantitative proteomics approaches have been used to investigate callus development, somatic embryogenesis, organogenesis, and stress response in numbers of plant species. However, they have not been applied to callus from monocotyledonous halophyte alkaligrass (Puccinellia tenuifora). Results: The alkaligrass callus growth, viability and membrane integrity were perturbed by 50 mM and 150 mM NaCl treatments. Callus cells accumulated the proline, soluble sugar and glycine betaine for the maintenance of osmotic homeostasis. Importantly, the activities of ROS scavenging enzymes (e.g., SOD, APX, POD, GPX, MDHAR and GR) and antioxidants (e.g., ASA, DHA and GSH) were induced by salinity. The abundance patterns of 55 salt-responsive proteins indicate that salt signal transduction, cytoskeleton, ROS scavenging, energy supply, gene expression, protein synthesis and processing, as well as other basic metabolic processes were altered in callus to cope with the stress. Conclusions: The undifferentiated callus exhibited unique salinity-responsive mechanisms for ROS scavenging and energy supply. Activation of the POD pathway and AsA-GSH cycle was universal in callus and differentiated organs, but salinity-induced SOD pathway and salinity-reduced CAT pathway in callus were different from those in leaves and roots. To cope with salinity, callus mainly relied on glycolysis, but not the TCA cycle, for energy supply.

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Hydrogen Peroxide Response in Leaves of Poplar (Populus simonii × Populus nigra) Revealed from Physiological and Proteomic Analyses

Cited by 26 publications

References 73 publications

Comparative Proteomic Analysis of Nodulated and Non-Nodulated Casuarina glauca Sieb. ex Spreng. Grown under Salinity Conditions Using Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)

Comparative Proteomic Analysis of Nodulated and Non-Nodulated Casuarina glauca Sieb. ex Spreng. Grown under Salinity Conditions Using Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS)

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

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