Hydrogen peroxide spraying alleviates drought stress in soybean plants

Ishibashi, Yoshihiro; Yamaguchi, Hikoyuki; Yuasa, Takashi; Iwaya‐Inoue, Mari; Arima, Susumu; 鄭, 紹輝

doi:10.1016/j.jplph.2011.02.003

Cited by 145 publications

(82 citation statements)

References 44 publications

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“…Relative water content and leaf water potential are the most important indicators of drought stress in plants and can be considered as an index for drought tolerance [29]. It is believed that H 2 O 2 has the ability to improve plant water status [17,33]. The results of the present study showed that RWC and Ψ leaf declined in response to drought stress.…”

Section: Discussionmentioning

confidence: 52%

“…Thus it could be deduced that H 2 O 2 contributed to the stress acclimation of soybean cultivars. In a similar study, Ishibashi et al [17] showed that RWC of drought-stressed leaves pretreated with H 2 O 2 were higher than that of leaves pre-treated with distilled water. In addition, the same authors reported that exogenous application of H 2 O 2 caused an increase in the mRNA levels of key enzymes for the biosynthesis of oligosaccharides, which are known to help plants tolerate drought stress.…”

Section: Discussionmentioning

confidence: 83%

“…Indeed, literature contains numerous evidence that H 2 O 2 is an essential signal in mediating stomatal closure induced by ABA under a variety of abiotic stresses, such as drought and salinity [18,26,27]. However, Ishibashi et al [17] reported that leaf water retention by H 2 O 2 spraying was probably due to the increased oligosaccharide biosynthesis rather than rapid stomatal closure.…”

Section: Discussionmentioning

confidence: 99%

“…Several recent studies have demonstrated that exogenous H 2 O 2 can enhance abiotic stress tolerance either by modulating ROS detoxification or regulating multiple stress-responsive pathways and gene expression [1,15,21,33]. Ishibashi et al [17] showed that exogenous application of H 2 O 2 promotes the up-regulation of stress responsive genes and increases drought stress tolerance. Lately a micro-array study indicated that the expression level of several hundred genes altered in H 2 O 2 treated Arabidopsis cell cultures [7].…”

Section: Introductionmentioning

confidence: 99%

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Exogenous low-dose hydrogen peroxide enhances drought tolerance of soybean (Glycine maxL.) through inducing antioxidant system

Güler

Pehlivan

2016

Acta Biologica Hungarica

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Hydrogen peroxide (H 2 O 2 ) functions as a signal molecule in plants under abiotic and biotic stress. In this study, the role of exogenous H 2 O 2 in improving drought tolerance in two soybean cultivars (Glycine max L. Merrill) differing in their tolerance to drought was evaluated. Plants were grown in plastic pots with normal irrigation in a phytotron. Four weeks after radicle emergence, either 1 mM H 2 O 2 or distilled water was sprayed as foliar onto the leaves of each plant, after drought stress was applied. Leaf samples were harvested on the 4 th and 7 th days of the drought. Antioxidant-related enzyme activity, such as the superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) content was measured during the drought period. Drought stress decreased leaf water potential, relative water content and photosynthetic pigment content but enhanced lipid peroxidation and endogenous H 2 O 2 concentration. By contrast, exogenous low dose H 2 O 2 improved water status, pigment content and lipid peroxidation under drought stress. Endogenous H 2 O 2 concentration was reduced by exogenous H 2 O 2 as compared to drought treatment alone. H 2 O 2 pre-treatment induced all the antioxidant enzyme activities, to a greater extent than the control leaves, during drought. H 2 O 2 pretreatment further enhanced the activities of antioxidant enzymes in the tolerant cultivar compared to the sensitive cultivar. Results suggested that low dose H 2 O 2 pre-treatment alleviated water loss and H 2 O 2 content and increased drought stress tolerance by inducing the antioxidant system.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exogenous low-dose hydrogen peroxide enhances drought tolerance of soybean (Glycine maxL.) through inducing antioxidant system

Güler

Pehlivan

2016

Acta Biologica Hungarica

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“…Moreover, it has been reported that the increase of H 2 O 2 in the cells of some drought-resistant plants might be more related to a structural role than to oxidative damage (Munné-Bosch et al, 2001;Jubany-Marí et al, 2009). In addition, a number of studies focused on physiological function of exogenous H 2 O 2 which enabled plants to tolerate many kinds of abiotic stresses (Li et al, 2009;Gao et al, 2010;Ishibashi et al, 2011;Zhang et al, 2011). ROS is considered to be a part of stress-induced signal transduction, and H 2 O 2 regulates numerous expressions of genes and complex signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Peroxide Acts as a Signaling Molecule for the Methyl Jasmonate-Induced Antioxidant Defense in Wheat Callus to Promote Enhanced Drought Tolerance

Ma¹,

Wang²,

Sun³

et al. 2015

JAS

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Drought stress is a major challenge in agriculture, causing severe loss of crops throughout the world. Methyl jasmonate (MeJA) regulates a variety of plant developmental process and responds to many biotic and abiotic stresses. To explore the physiological mechanisms of drought stress mitigated by exogenous MeJA, we subjected callus of wheat (Triticum aestivum L.) to drought induced medium after pretreatment with 10 ml sterile solution that contained the following singly or in combinations: MeJA (0, 0.25, and 2.5µM) and the lipoxygenase (LOX) inhibitor salicylhydroxamic acid (SHAM; 0 and 0.5 mM) for 24 hours. The endogenous jasmonic acid (JA) content, activity of LOX, hydrogen peroxide (H 2 O 2 ) content, antioxidant enzymes activities, levels of reactive oxygen species (ROS), content of malondialdehyde (MDA), and cell viability were measured. The results showed that exogenous MeJA induced the activity of LOX and resulted in rapid increase in endogenous JA levels in a concentration-dependent manner; JA declined gradually after reaching its maximum. The level of H 2 O 2 increased with increases in LOX activity and endogenous JA level, which are involved in the octadecanoid signaling pathway. However, SHAM inhibited the LOX activity, the endogenous JA level, and the accumulation of H 2 O 2 in wheat callus. In addition, the activities of several antioxidant enzymes increased after MeJA-pretreatment, but this effect was also inhibited by SHAM. The cumulative results suggest that H 2 O 2 was generated through the octadecanoid signaling pathway, and some antioxidant defense genes were provoked by the accumulation of H 2 O 2 . Therefore, we inferred that H 2 O 2 might act as a signaling molecule for the MeJA-induced antioxidant defense. Drought stress can be improved by MeJA-pretreatment, leading to decrease in endogenous MDA and ROS contents, which are normally induced by drought conditions. Both drought stress and exogenous MeJA-pretreatment increased in LOX activity, endogenous JA level, and antioxidant enzymes. Combinatorial treatments showed an apparent synergistic effect on the activities of these antioxidant enzymes and resulted in improved cell viability. These results confirmed the hypothesis that H 2 O 2 acts as a signaling molecule for the MeJA-induced antioxidant defense and could alleviate the negative effects of drought stress on wheat callus.

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Global explicit profiling of water deficit‐induced diminutions in agricultural crop sustainability

Singh

Tripathi

Dubey

et al. 2016

Water Stress and Crop Plants

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Hydrogen peroxide spraying alleviates drought stress in soybean plants

Cited by 145 publications

References 44 publications

Exogenous low-dose hydrogen peroxide enhances drought tolerance of soybean (Glycine maxL.) through inducing antioxidant system

Exogenous low-dose hydrogen peroxide enhances drought tolerance of soybean (Glycine maxL.) through inducing antioxidant system

Hydrogen Peroxide Acts as a Signaling Molecule for the Methyl Jasmonate-Induced Antioxidant Defense in Wheat Callus to Promote Enhanced Drought Tolerance

Global explicit profiling of water deficit‐induced diminutions in agricultural crop sustainability

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