Hydrogen sulfide promotes osteogenic differentiation of human periodontal ligament cells via p38-MAPK signaling pathway under proper tension stimulation

Jiang, Zhaoxia; Hua, Yi-Ming

doi:10.1016/j.archoralbio.2016.08.008

Cited by 21 publications

(18 citation statements)

References 33 publications

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“…Other non-collagenous proteins, such as laminin 5 and matrix protein 3, can induce the expression of Cbfa-1 and ALP through the ERK1/2 signaling pathway and can enhance the matrix mineralization and osteogenic differentiation of hMSCs. 408 Together, these results suggest that the MAPK signaling pathway plays an important role in the osteogenic differentiation of hMSCs.…”

Section: Interactions Between Bone Biomaterials and Stem Cellsmentioning

confidence: 80%

Bone biomaterials and interactions with stem cells

et al. 2017

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Bone biomaterials play a vital role in bone repair by providing the necessary substrate for cell adhesion, proliferation, and differentiation and by modulating cell activity and function. In past decades, extensive efforts have been devoted to developing bone biomaterials with a focus on the following issues: (1) developing ideal biomaterials with a combination of suitable biological and mechanical properties; (2) constructing a cell microenvironment with pores ranging in size from nanoscale to submicro- and microscale; and (3) inducing the oriented differentiation of stem cells for artificial-to-biological transformation. Here we present a comprehensive review of the state of the art of bone biomaterials and their interactions with stem cells. Typical bone biomaterials that have been developed, including bioactive ceramics, biodegradable polymers, and biodegradable metals, are reviewed, with an emphasis on their characteristics and applications. The necessary porous structure of bone biomaterials for the cell microenvironment is discussed, along with the corresponding fabrication methods. Additionally, the promising seed stem cells for bone repair are summarized, and their interaction mechanisms with bone biomaterials are discussed in detail. Special attention has been paid to the signaling pathways involved in the focal adhesion and osteogenic differentiation of stem cells on bone biomaterials. Finally, achievements regarding bone biomaterials are summarized, and future research directions are proposed.

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Section: Interactions Between Bone Biomaterials and Stem Cellsmentioning

confidence: 80%

Bone biomaterials and interactions with stem cells

et al. 2017

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“…Then, we investigated how POSTN improves migration and osteogenic differentiation of PDLSCs under inflammatory conditions.Previous studies have reported that POSTN promotes cell mobility, adhesion and survival in a number of cell types by binding to integrin αvβ3 and αvβ5 via focal adhesion kinase (FAK) phosphorylation and AKT phosphorylation in the integrin-mediated signalling pathway [23][24][25]. According to previous research, the differentiation and inflammatory response of PDLSCs or PDL cells could be affected through the P38 and ERK1/2 signalling pathways by a number of stimulatory factors [31][32][33][34]. Additionally, our previous study found thatPOSTN acted on PDLSCs via the integrin signalling pathway; the integrin inhibitor Cilengitide represses POSTN-enhanced cell migration.…”

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confidence: 99%

Periostin promotes migration and osteogenic differentiation of human periodontal ligament mesenchymal stem cells via the Jun amino‐terminal kinases (JNK) pathway under inflammatory conditions

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“…Our previous in vitro study also demonstrated that H 2 S could promote osteogenic differentiation by up-regulating the expression ratio of OPG/RANKL expression in human PDLSCs ( 18 ). It was also demonstrated that H 2 S significantly increased the expression of ALP, OCN, runt-related transcription factor 2 and collagen 1 in the human PDLSCs with tension force stimulation, and promoted osteogenic differentiation of human PDLSCs by activating the p38-MAPK and ERK signaling pathways ( 19 , 35 ). In the present study, exogenous H 2 S could up-regulate the expression of ALP and OCN, the ratio of RANKL/OPG and the number of osteoclasts in the alveolar bone.…”

Section: Discussionmentioning

confidence: 95%

“…In the present study, exogenous H 2 S could up-regulate the expression of ALP and OCN, the ratio of RANKL/OPG and the number of osteoclasts in the alveolar bone. ALP has been commonly studied as an early marker of osteogenic differentiation in tension force experiments and OCN is a key transcription factor in the modulation of osteogenic differentiation and bone formation ( 19 ). The results of the present study revealed that H 2 S could up-regulate the process of osteogenesis, osteoclastic activities and bone remodeling.…”

Section: Discussionmentioning

confidence: 99%

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Hydrogen sulfide regulates bone remodeling and promotes orthodontic tooth movement

Hua

2017

Molecular Medicine Reports

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Hydrogen sulfide (H2S) is a gas signaling molecule that has multiple influences on physiological and pathological processes in the mammalian body, including vasodilation, neurotransmission, inflammation, hypoxia sensing and bone remodeling. Our previous studies suggested that H2S might be involved in the periodontal tissue remodeling during the orthodontic tooth movement (OTM) via increasing periodontal ligament cell differentiation, tissue mineralization, bone formation and collagen synthesis. The aim of the present study was to investigate the effects of H2S on alveolar bone remodeling that is associated with tooth movement. Experiments were performed in an OTM mouse model. Sodium hydrosulfide (NaHS), which is a donor of H2S and DL-propargylglycine (PAG) and a cystathionine-γ-lyase (CSE) inhibitor, which could also decrease H2S expression, were administered intraperitoneally and respectively. A total of 60 male C57BL6/J mice were divided into 4 groups; Control, NaHS, PAG and combination (PAG+NaHS). The rate of OTM and the bone mineral density (BMD) of alveolar bone were scanned and measured by micro-computed tomography (micro-CT). The number of osteoclasts and expression of the tumor necrosis factor ligand superfamily member-11 (RANKL), alkaline phosphatase (ALP), osteocalcin (OCN) and osteoprotegerin (OPG) in alveolar bone were accessed to evaluate the osteoclastic activity and osteogenesis with histochemistry of tartrate-resistant acid phosphatase staining, immunohistochemistry and reverse transcription-quantitative polymerase chain reaction. In the alveolar bone, NaHS increased the OTM and decreased the BMD, respectively. PAG significantly decrease OTM and increased the BMD. NaHS combined with PAG rescued the PAG-induced changes in the OTM and the BMD. Additionally, the number of osteoclasts, the expression of RANKL, ALP, OCN and the ratio of RANKL/OPG were significantly up-regulated in the NaHS group. In contrast, PAG down-regulated the number of osteoclasts, the expression of RANKL, ALP, OCN and the ratio of RANKL/OPG. These findings suggested that H2S might facilitate the OTM by enhancing alveolar bone remodeling as a result of an increased osteoclastic activity and osteogenesis.

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Hydrogen sulfide promotes osteogenic differentiation of human periodontal ligament cells via p38-MAPK signaling pathway under proper tension stimulation

Abstract: HS could promote osteogenic differentiation of hPDLCs by activating p38-MAPK and ERK signaling pathways.

Cited by 21 publications

References 33 publications

Bone biomaterials and interactions with stem cells

Bone biomaterials and interactions with stem cells

Periostin promotes migration and osteogenic differentiation of human periodontal ligament mesenchymal stem cells via the Jun amino‐terminal kinases (JNK) pathway under inflammatory conditions

Hydrogen sulfide regulates bone remodeling and promotes orthodontic tooth movement

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