Hydrogen Sulfide Protects Retinal Ganglion Cells Against Glaucomatous Injury In Vitro and In Vivo

Liu, Hanhan; Anders, Fabian; Thanos, Solon; Mann, Carolina; Liu, Aiwei; Grus, Franz H.; Pfeiffer, Norbert; Prokosch‐Willing, Verena

doi:10.1167/iovs.17-22200

Cited by 38 publications

(38 citation statements)

References 27 publications

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“…Zur Darstellung der Gefäße der Retina wurden kurz vor der Kauterisation der Tiere OCT-Aufnahmen (OCT: optische Kohärenztomografie) des operierten Auges mit dem Spectral-Domain-OCT (SD-OCT; Heidelberg Engineering; Heidelberg) angefertigt, wie in folgenden Studien zuvor [20,21]. Damit die Darstellung der Gefäße optimal ist, trugen alle Ratten während der Untersuchung entsprechend angepasste Sammellinsen.…”

Section: Oct-untersuchungunclassified

Erhöhter Augeninnendruck für 7 Wochen induziert lokale Gefäßveränderungen im experimentellen Glaukommodell in vivo

Mann

Anders

Liu

et al. 2018

Klin Monatsbl Augenheilkd

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Section: Oct-untersuchungunclassified

Erhöhter Augeninnendruck für 7 Wochen induziert lokale Gefäßveränderungen im experimentellen Glaukommodell in vivo

Mann

Anders

Liu

et al. 2018

Klin Monatsbl Augenheilkd

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“…Recently, there have been questions whether any such effect of H 2 S could be applicable in glaucoma. Therefore it has been tested in some previous studies …”

Section: Introductionmentioning

confidence: 99%

“…Therefore it has been tested in some previous studies. [16][17][18][19][20] Apoptosis is essential in normal development for the maintenance of tissue homeostasis. 21,22 The process of apoptosis induction mechanism is initiated by several factors, including decrease in growth factors and DNA damage.…”

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confidence: 99%

The effects of intravitreal H₂S application on apoptosis in the retina and cornea in experimental glaucoma model

Erişgin

Özer

Tosun

et al. 2019

Int J Experimental Path

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Summary One of the most important causes of visual loss (blindness) is glaucoma, which occurs due to the degeneration of the ganglion cells in retina. It has been shown that hydrogen sulphide (H2S) acts an antioxidant, neuroprotective and neuromodulator and provides protection against oxidative stress and apoptosis. This study aims to examine through which apoptotic pathway H2S acts in experimental glaucoma model. Twenty‐two male wistar albino rats were used in this study. Group 1 (n = 6, control group): Intravitreal saline was given in the third week without inducing ocular hypertension (OHT) with laser photocoagulation. Group 2 (n = 8): After the induction of OHT with laser photocoagulation, intravitreal saline was given in the third week. Group 3 (n = 8): After the induction of OHT with laser photocoagulation, intravitreal H2S’s donor sodium hydrosulphide (NaSH) 100 nmol/L was given in the third week. At the end of the 6th week, the eyes of the rats were sacrified under anaesthesia and extracted and then routine tissue follow‐up was undertaken. Besides haematoxylin & eosin (H&E) staining, Bax, Bcl‐2, p53 and caspase‐3 activation were examined immunohistochemically in the retina and the cornea. This showed that ocular hypertension caused apoptosis through the intrinsic pathway, due to Bax and caspase‐3 activation, in both retina and cornea, and that this led to DNA damage due to p53 activation. Also, we found that H2S exposure in glaucoma distinctly suppressed Bax, caspase‐3 and p53 activations in retina but that it has a limited effect on the cornea. According to these results, glaucoma caused apoptosis in the retina through intrinsic pathway, and the damage to the retina could be compensated partially by H2S but would have limited on the cornea.

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“…Alteration of endogenous H 2 S level in retina is correlated with different pathological situations, and its exogenous donors exhibited potential in protecting retinal ganglion cells against assaults, such as diabetic retinopathy, ischemia-reperfusion injury, and N-methyl-Daspartic acid-(NMDA-) induced excitatory neurotoxicity [16][17][18]. In our previous study, alteration of endogenous H 2 S synthases is observed in a glaucoma animal model; furthermore, we observed that GYY4137, a slow-release H 2 S donor, effectively protected RGCs against different glaucomatous injuries in vitro and in vivo [19].…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Sulfide and β-Synuclein Are Involved and Interlinked in the Aging Glaucomatous Retina

Liu

Mercieca

Anders

et al. 2020

Journal of Ophthalmology

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Purpose. Glaucoma, one of the leading causes of irreversible blindness worldwide, is a group of disorders characterized by progressive retinal ganglion cell (RGC) loss. Synucleins, a family of small proteins, have been of interest in studies of neurodegeneration and CNS. However, their roles and functions in glaucoma are still not completely understood and remain to be explored. Our previous studies showed that α-synuclein and H2S play a pivotal role in glaucoma. This study aims to (1) elucidate the potential roles and functions of synucleins in glaucoma throughout aging, (2) investigate the interaction between the synucleins and H2S, and better understand the mechanism of H2S in neuroprotection. Methods. The chronic IOP elevation model was carried out in 12 animals at different ages (3 months and 14 months), and RGCs were quantified by Brn3a staining. Mass spectrometric-assisted proteomics analysis was employed to measure synuclein levels and H2S producing proteins in retina. Secondly, the acute IOP elevation model was carried out in 12 juvenile animals, with or without intravitreal injection of GYY4137 (a H2S donor). RGCs were quantified along with the abundancy of synucleins. Results. RGCs and β-synuclein (SNCB) are significantly changed in old animals. Under chronic IOP elevation, there is a significant RGC loss in old animals, whereas no significant change in young animals; SNCB is significantly downregulated and 3MST is significantly upregulated in young animals due to IOP, while no significant changes in old ones are notable. Under acute IOP elevation (approx. 55 mmHg), a significant RGC loss is observed; exogenous H2S significantly reduced RGC loss and downregulated SNCB levels. Conclusion. The present study indicates a strong link between ageing and SNCB regulation. In young animals SNCB is downregulated going along with less RGC loss. Furthermore, increasing endogenous H2S is effective to downregulate SNCB and is neuroprotective against acute IOP elevation.

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Hydrogen Sulfide Protects Retinal Ganglion Cells Against Glaucomatous Injury In Vitro and In Vivo

Abstract: H2S is specifically regulated in experimental glaucoma. By scavenging reactive oxygen species and dilating retinal vessels, H2S may protect RGCs from pressure and oxidative stress-induced RGC loss in vitro and in vivo. Therefore, H2S might be a novel neuroprotectant in glaucoma.

Cited by 38 publications

References 27 publications

Erhöhter Augeninnendruck für 7 Wochen induziert lokale Gefäßveränderungen im experimentellen Glaukommodell in vivo

Erhöhter Augeninnendruck für 7 Wochen induziert lokale Gefäßveränderungen im experimentellen Glaukommodell in vivo

The effects of intravitreal H₂S application on apoptosis in the retina and cornea in experimental glaucoma model

Hydrogen Sulfide and β-Synuclein Are Involved and Interlinked in the Aging Glaucomatous Retina

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Hydrogen Sulfide Protects Retinal Ganglion Cells Against Glaucomatous Injury In Vitro and In Vivo

Abstract: H2S is specifically regulated in experimental glaucoma. By scavenging reactive oxygen species and dilating retinal vessels, H2S may protect RGCs from pressure and oxidative stress-induced RGC loss in vitro and in vivo. Therefore, H2S might be a novel neuroprotectant in glaucoma.

Cited by 38 publications

References 27 publications

Erhöhter Augeninnendruck für 7 Wochen induziert lokale Gefäßveränderungen im experimentellen Glaukommodell in vivo

Erhöhter Augeninnendruck für 7 Wochen induziert lokale Gefäßveränderungen im experimentellen Glaukommodell in vivo

The effects of intravitreal H2S application on apoptosis in the retina and cornea in experimental glaucoma model

Hydrogen Sulfide and β-Synuclein Are Involved and Interlinked in the Aging Glaucomatous Retina

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The effects of intravitreal H₂S application on apoptosis in the retina and cornea in experimental glaucoma model