Hydrolysis of surimi wastewater for production of transglutaminase by Enterobacter sp. C2361 and Providencia sp. C1112

Protein-peptide nutritional material prepared from surimi wash-water using immobilized chymotrypsin-trypsin

“…WSP (2.257 ± 0.009 mg mL −1 ) mainly consisted of sarcoplasmic protein in hairtail surimi wash‐water, and NPN (1.923 ± 0.010 mg mL −1 ) mainly contained oligopeptides and free amino acids, which are flavoring compounds and flavoring precursors for food. Other workers have produced surimi wash‐waters with protein concentrations falling in the range 0.8 − 16 mg mL −1 , which involved multiple washing steps. The value reported in this work (6.78 mg mL −1 ) was within this range.…”

“…This process produces large quantities of wastewater . Surimi wash‐water typically contains 15–30% of the initial protein in fish, resulting in considerable waste of protein resources . The annual output of surimi amounts to 200 000 tons, generating more than 6000 tons of protein resource waste.…”

Mini Review: Pemanfaatan Microbial Transglutaminase (MTGase) pada Surimi Ikan Patin

“…Industri surimi menghasilkan banyak limbah. Limbah-limbah ini masih belum banyak dimanfaatkan dan dapat menimbulkan masalah lingkungan (H-Kittikun et al, 2012). Air limbah surimi mengandung banyak nutrisi.…”

Microbial Transglutaminase: Source, Production and Its Role to Improve Surimi Properties

“…Other MTGase microbial producers include Bacillus substilis (Kobayashi et al, 1998), Zygomonas mobilis and Hafnea alvei (Bech et al, 2002). Kittikun et al (2012) reported Enterobacter sp. C2361 and Providencia sp.…”

“…Since it was published by Ando et al (1989), the medium composition for MTGase productions from Streptomyces has been almost the same which contains yeast extract, peptone, sodium phosphate, potassium phosphate, magnesium sulphate and a carbon source (Portilla- Rivera et al, 2009;Bahrim et al, 2010;Aidaroos et al, 2011;Zheng et al, 2001).The common carbon sources used are glucose (Bahrim et al, 2010), starch (Zheng et al, 2001), solubilized starch (Kittikun et al, 2012;Bourneow et al, 2012) anddextrins (Guerra-Rodríguez & Vazquez, 2013b). However, to be economically acceptable for industrialscale, the source of culture medium should not only abundant but also cheap.The formulation of culture medium was crucially importance due to its effect on yield and volumetric productivity (Bahrim et al, 2010;Portilla-Rivera et al, 2009).The cost for a microbial enzyme production represented almost 30% by fermentation medium (Portilla- Rivera et al, 2009).The medium commonly used for transglutaminase production from Streptomycesare not economically attractiv e. It contains expensiv e f or medium components i.e.…”