Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracellular matrix environment for human dermal fibroblasts

Abstract

We have found that a water-soluble alkaline-digested form of eggshell membrane (ASESM) can provide an extracellular matrix (ECM) environment for human dermal fibroblast cells (HDF) in vitro. Avian eggshell membrane (ESM) has a fibrous-meshwork structure and has long been utilized as a Chinese medicine for recovery from burn injuries and wounds in Asian countries. Therefore, ESM is expected to provide an excellent natural material for biomedical use. However, such applications have been hampered by the insolubility of ESM proteins. We have used a recently developed artificial cell membrane biointerface, 2-methacryloyloxyethyl phosphorylcholine polymer (PMBN) to immobilize ASESM proteins. The surface shows a fibrous structure under the atomic force microscope, and adhesion of HDF to ASESM is ASESM-dose-dependent. Quantitative mRNA analysis has revealed that the expression of type III collagen, matrix metalloproteinase-2, and decorin mRNAs is more than two-fold higher when HDF come into contact with a lower dose ASESM proteins immobilized on PMBN surface. A particle-exclusion assay with fixed erythrocytes has visualized secreted water-binding molecules around the cells. Thus, HDF seems to possess an ECM environment on the newly designed PMBN-ASESM surface, and future applications of the ASESM-PMBN system for biomedical use should be of great interest.

Eggshell membrane ameliorates hepatic fibrogenesis in human C3A cells and rats through changes in PPARγ-Endothelin 1 signaling

“…Due to properties such as its high surface area, porous structure, inert nature, and water permeability, ESM has been used in metallurgy and bioremediation applications such as the recovery of gold from electroplating waste water3 and platforms for enzyme immobilization4. Notably, these studies did not deal with the food applications of ESM, although ESM contains high amounts of collagenized fibrous proteins.…”

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

“…Nanofibers made up of bovine serum albumin (BSA) have potential application in wound closure suturing materials as these nanofibers are highly biocompatible and biodegradable [233]. Avian egg shell membrane which is a meshwork of fibrous proteins, is used in the treatment of wounds and burn injuries as they provide an ECM environment for human dermal fibroblast cells [234]. Gastric pentadecapeptide has been used topically (cream) or systemically [intra-peritoneal (i.p.)…”

Pengaruh Aplikasi Gel Ekstrak Membran Kulit Telur Bebek 10% Terhadap Kepadatan Serabut Kolagen Pada Proses Penyembuhan Luka Gingiva

“…9 Sumber bahan baku cangkang telur bebek tersedia cukup banyak dan saat ini belum dimanfaatkan. Oleh sebab itu, pemanfaatan cangkang telur bebek merupakan salah satu bentuk usaha yang cukup relevan untuk meningkatkan nilai ekonomi dan mengurangi limbah yang ada di lingkungan.…”