Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells

Raabe, Oksana; Reich, Christine Maria; Wenisch, Sabine; Hild, Anne; Burg‐Roderfeld, Monika; Siebert, Hans Christian; Arnhold, Stefan

doi:10.1007/s00418-010-0760-4

Cited by 69 publications

(65 citation statements)

References 42 publications

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“…These fi ndings demonstrated that hydrolyzed fi sh collagen alone has the potential to induce and maintain ADSCs-derived chondrogenesis. These results support the application of ADSCs in equine veterinary tissue engineering, especially for cartilage repair," (Raabe et al 2010 ).…”

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confidence: 52%

“…Recently, Raabe et al ( 2010 ) "investigated the effect of transforming growth factor beta 1 (TGF-β1), in comparison to hydrolyzed fi sh collagen, in terms of the chondrogenic differentiation potential of Adipose-derived stromal cells (ADSCs). Adipose-derived stromal cells are multipotent cells which, in the presence of appropriate stimuli, can differentiate into various lineages such as the osteogenic, adipogenic and chondrogenic lines.…”

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confidence: 99%

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Marine Structural Proteins in Biomedicine and Tissue Engineering

Ehrlich

2014

Biological Materials of Marine Origin

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Marine biopolymers like collagens, gelatins, keratins (keratin-like proteins), and elastins (elastin-like proteins) have been investigated during the last decade as potentially approvable, manufacturable, highly reproducible, approvable, and affordable biological materials. The development of biocompatible composites and vehicles of marine biopolymer origin for growth, retention, delivery, and differentiation of stem cells is of crucial importance for regenerative medicine.All the marine structural proteins discussed have above possess high biomimetic potential. They either directly meet current challenges in many applied fi elds; or may be used as a source of bioinspiration for designing biomimetic materials and composites with hierarchical structure and appropriative mechanical properties. The application of new biomaterial technologies based on marine biopolymers offers the potential to direct the stem cell fate, targeting the delivery of cells and reducing immune rejection, thereby supporting the development of regenerative medicine (Armentano et al. 2013 ). The fi rst goal is to develop porous biodegradable composite scaffolds, which can be designed with initial properties that reproduce the tension-compression nonlinearity, viscoelasticity and anisotropy of different tissues by introducing specifi c nanostructures. The second goal is more complex, as it deals with development of artifi cial organs. However, the fi rst attempts have been carried out. Recently, dense and porous synthetic scaffolds were used as template for fi sh cells line, which, being attached, can proliferate and express fi sh skin components. This biomimetic approach has been used for development of a biosynthetic fi sh skin in vitro with future application in aquatic robots that can emulate living fi sh (Pouliot et al. 2004 ).As reported above, marine collagens of vertebrate origin as well as gelatins as their derivatives are the most popular biological materials. These can be used as alternative to terrestrial mammal's collagens especially in tissue engineering. According to Glowacki and Mizuno ( 2008 ), "there are two major approaches to tissue engineering for regeneration of tissues and organs. One involves cell-free materials and/or factors and one involves delivering cells to contribute to the regeneration process. Of the many scaffold materials being investigated, collagen type I, with selective removal of its telopeptides, has been shown to have many advantageous features for both of these approaches," (Glowacki and Mizuno 2008 ).

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supporting

confidence: 52%

mentioning

confidence: 99%

Marine Structural Proteins in Biomedicine and Tissue Engineering

Ehrlich

2014

Biological Materials of Marine Origin

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“…25 According to FIGURE 6. images of high-speed AFM of tilapia and porcine collagen undergoing fibril formation at 0, 11, and 15 min after the addition of collagen solution to PBS (2).…”

Section: Discussionmentioning

confidence: 98%

Rapid oriented fibril formation of fish scale collagen facilitates early osteoblastic differentiation of human mesenchymal stem cells

Matsumoto

Uemura

et al. 2014

J Biomedical Materials Res

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We studied the effect of fibril formation of fish scale collagen on the osteoblastic differentiation of human mesenchymal stem cells (hMSCs). We found that hMSCs adhered easily to tilapia scale collagen, which remarkably accelerated the early stage of osteoblastic differentiation in hMSCs during in vitro cell culture. Osteoblastic markers such as ALP activity, osteopontin, and bone morphogenetic protein 2 were markedly upregulated when the hMSCs were cultured on a tilapia collagen surface, especially in the early osteoblastic differentiation stage. We hypothesized that this phenomenon occurs due to specific fibril formation of tilapia collagen. Thus, we examined the time course of collagen fibril formation using high-speed atomic force microscopy. Moreover, to elucidate the effect of the orientation of fibril formation on the differentiation of hMSCs, we measured ALP activity of hMSCs cultured on two types of tilapia scale collagen membranes with different degrees of fibril formation. The ALP activity in hMSCs cultured on a fibrous collagen membrane was significantly higher than on a non-fibrous collagen membrane even before adding osteoblastic differentiation medium. These results showed that the degree of the fibril formation of tilapia collagen was essential for the osteoblastic differentiation of hMSCs.

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“…Both molecules were used to analyse the applicability of adipose-derived stromal cells (ADSCs) in chondral defects (Raabe et al 2010). TGF-1 and hydrolyzed Wsh collagen were found to be very eVective stimulants for diVerentiation of the chondrogenic lineage making ADSCs in equine veterinary tissue engineering useable in cartilage repair.…”

Section: Developmentmentioning

confidence: 99%

Histochemistry and cell biology: the annual review 2010

Hübner

Efthymiadis

2011

Histochem Cell Biol

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This review summarizes recent advances in histochemistry and cell biology which complement and extend our knowledge regarding various aspects of protein functions, cell and tissue biology, employing appropriate in vivo model systems in conjunction with established and novel approaches. In this context several non-expected results and discoveries were obtained which paved the way of research into new directions. Once the reader embarks on reading this review, it quickly becomes quite obvious that the studies contribute not only to a better understanding of fundamental biological processes but also provide use-oriented aspects that can be derived therefrom.

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Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells

Cited by 69 publications

References 42 publications

Marine Structural Proteins in Biomedicine and Tissue Engineering

Marine Structural Proteins in Biomedicine and Tissue Engineering

Rapid oriented fibril formation of fish scale collagen facilitates early osteoblastic differentiation of human mesenchymal stem cells

Histochemistry and cell biology: the annual review 2010

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