Hydrolyzed polyacrylamide biotransformation in an up-flow anaerobic sludge blanket reactor system: key enzymes, functional microorganisms, and biodegradation mechanisms

Zhao, Lanmei; Song, Tianwen; Han, Dong; Bao, Mutai; Lu, Jinren

doi:10.1007/s00449-019-02094-w

Cited by 26 publications

(16 citation statements)

References 45 publications

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“…Also, amidase activity was demonstrated to be responsible for transforming PAAm into PAA during anaerobic biodegradation with a w a s t e w a t e r m i c r o b i a l co m m u n i t y t h a t i n cl u de s Bacteriodetes, Firmicutes, Proteobacteria, Spirochaetes, and others (Dai et al 2015). Also, urease activity has been correlated with HPAAm degradation in an upflow anaerobic sludge blanket reactor system, where Proteobacteria, Chloroflexi, Bacteriodetes, Fusobacteria, and others were identified (Zhao et al 2019). The significant role of ureases on HPAAm biodegradation was demonstrated by enzyme inhibition experiments in aerobic cultures of Bacillus megaterium SZK-5.…”

Section: Amidasesmentioning

confidence: 97%

“…3a). Enzymatic activities such as amidases, nitriledegrading enzymes, esterases, and lipases have been responsible for AP side group modifications (Tauber et al 2000;Mahalik and Madras 2005;Fischer-Colbrie et al 2006;Zhao et al 2019;Gitalis et al 2019: Huang et al 2018.…”

Section: First Phase: Enzymatic Elimination Of Side Groupsmentioning

confidence: 99%

“…Similar results were observed for HPAAm fermentation by an activated sludge microbial community integrated by Proteobacteria, Bacteriodetes, and Chloroflexi, among others, in which dehydrogenase and urease activities correlated with HPAAm biodegradation. The hydrolysis of C-N by ureases, followed by the participation of dehydrogenases and monooxygenases (non-demonstrated), producing acetyl-CoA, pyruvic acid, and organic acids (acetic, propionic, and butyric) as biodegradation products, was proposed (Zhao et al 2019).…”

Section: Anaerobic Pathwaysmentioning

confidence: 99%

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Current status on the biodegradability of acrylic polymers: microorganisms, enzymes and metabolic pathways involved

Gaytán

Burelo

Loza-Tavera

2021

Appl Microbiol Biotechnol

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Acrylic polymers (AP) are a diverse group of materials with broad applications, frequent use, and increasing demand. Some of the most used AP are polyacrylamide, polyacrylic acid, polymethyl methacrylates, and polyacrylonitrile. Although no information for the production of all AP types is published, data for the most used AP is around 9 MT/year, which gives an idea of the amount of waste that can be generated after products' lifecycles. After its lifecycle ends, the fate of an AP product will depend on its chemical structure, the environmental setting where it was used, and the regulations for plastic waste management existing in the different countries. Even though recycling is the best fate for plastic polymer wastes, few AP can be recycled, and most of them end up in landfills. Because of the pollution crisis the planet is immersed, setting regulations and developing technological strategies for plastic waste management are urgent. In this regard, biotechnological approaches, where microbial activity is involved, could be attractive eco-friendly strategies. This mini-review describes the broad AP diversity, their properties and uses, and the factors affecting their biodegradability, underlining the importance of standardizing biodegradation quantification techniques. We also describe the enzymes and metabolic pathways that microorganisms display to attack AP chemical structure and predict some biochemical reactions that could account for quaternary carbon-containing AP biodegradation. Finally, we analyze strategies to increase AP biodegradability and stress the need for more studies on AP biodegradation and developing stricter legislation for AP use and waste control. Key points• Acrylic polymers (AP) are a diverse and extensively used group of compounds.• The environmental fates and health effects of AP waste are not completely known.• Microorganisms and enzymes involved in AP degradation have been identified.• More biodegradation studies are needed to develop AP biotechnological treatments.

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Section: Amidasesmentioning

confidence: 97%

Section: First Phase: Enzymatic Elimination Of Side Groupsmentioning

confidence: 99%

Section: Anaerobic Pathwaysmentioning

confidence: 99%

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Current status on the biodegradability of acrylic polymers: microorganisms, enzymes and metabolic pathways involved

Gaytán

Burelo

Loza-Tavera

2021

Appl Microbiol Biotechnol

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“…The subdominant phyla were Bacteroidetes (10.89 ± 6.31%) and Chlorobi (2.96 ± 1.12%). Members of Bacteroidetes can degrade residual recalcitrant substances and high-molecular-weight organic compounds [40,41].…”

Section: Taxonomic Complexity Of the Microbial Communitymentioning

confidence: 99%

Diversity and functional prediction of microbial communities involved in the first aerobic bioreactor of coking wastewater treatment system

et al. 2020

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The pre-aerobic process of coking wastewater treatment has strong capacity of decarbonization and detoxification, which contribute to the subsequent dinitrogen of non-carbon source/heterotrophic denitrification. The COD removal rate can reach > 90% in the first aerobic bioreactor of the novel O/H/O coking wastewater treatment system during long-term operation. The physico-chemical characteristics of influent and effluent coking wastewater in the first aerobic bioreactor were analyzed to examine how they correlated with bacterial communities. The diversity of the activated sludge microbial community was investigated using a culture-independent molecular approach. The microbial community functional profiling and detailed pathways were predicted from the 16S rRNA gene-sequencing data by the PICRUSt software and the KEGG database. High-throughput MiSeq sequencing results revealed a distinct microbial composition in the activated sludge of the first aerobic bioreactor of the O/H/O system. Proteobacteria, Bacteroidetes, and Chlorobi were the decarbonization and detoxification dominant phyla with the relative abundance of 84.07 ± 5.45, 10.89 ± 6.31, and 2.96 ± 1.12%, respectively. Thiobacillus, Rhodoplanes, Lysobacter, and Leucobacter were the potential major genera involved in the crucial functional pathways related to the degradation of phenols, cyanide, benzoate, and naphthalene. These results indicated that the comprehensive understanding of the structure and function diversity of the microbial community in the bioreactor will be conducive to the optimal coking wastewater treatment.

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“…The essence of biodegradation is enzymatic reaction, and the activity of key enzymes had been detected in the HPAM fermentation broth [13]. Zhang et al [7] found that the efficiencies of HPAM removal were positively correlated with the activity of dehydrogenase and urease, respectively.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation Mechanism of Anionic Polyacrylamide in Coal Preparation Using Molecular Simulation

Wang¹,

Zhang²,

Wu³

et al. 2021

Preprint

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Biodegradation of anionic polyacrylamide (HPAM) and polyacrylate (PAA) by key enzymes, such as amidase and bacterial laccase, have been reported. However, the interaction mechanism between HPAM or PAA and enzymes is still poorly unclear. Here, docking study was undertook to demonstrate the binding modes and interaction details for degradation of HPAM or PAA. Then, bioactivities between PAA and HPAM were compared with frontier orbital theory. The docking results showed that HPAM completely buried in pocket of Rhodococcus sp. N-771 amidase (Rh Amidase), while most of PAA molecule exposed outside pocket of Bacillus subtilis laccase ( B. subtilis laccase ), further suggesting PAA was much more difficult to degrade than HPAM. Hydrophobic interactions and hydrogen bonds were necessary for stabilizing HPAM-Rh Amidase or PAA- B. subtilis laccase complex. The frontier orbital analysis indicated that bioactivity of PAA was higher than that of PAA. These findings provide an insight into enzyme-catalyzed degradation of HPAM. It is helpful in designing highly efficient enzymes against HPAM or PAA to protect environment.

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Hydrolyzed polyacrylamide biotransformation in an up-flow anaerobic sludge blanket reactor system: key enzymes, functional microorganisms, and biodegradation mechanisms

Cited by 26 publications

References 45 publications

Current status on the biodegradability of acrylic polymers: microorganisms, enzymes and metabolic pathways involved

Current status on the biodegradability of acrylic polymers: microorganisms, enzymes and metabolic pathways involved

Diversity and functional prediction of microbial communities involved in the first aerobic bioreactor of coking wastewater treatment system

Biodegradation Mechanism of Anionic Polyacrylamide in Coal Preparation Using Molecular Simulation

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