Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for acidic herbicides and metabolites analysis in fresh water

Fauvelle, Vincent; Mazzella, Nicolás; Morin, Soizic; Moreira, Sylvia; Delest, Brigitte; Budzinski, Hélène

doi:10.1007/s11356-014-2876-x

Cited by 18 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three additional experimental units, used as abiotic positive controls, were filled with culture medium contaminated with the highest concentration of metolachlor. The three concentrations correspond to (i) environmentally realistic exposure concentrations (1 µg.L -1 ) in the Leyre watershed (Roubeix et al, 2012) or other rivers of the area (Fauvelle et al, 2014), and (ii) for the higher ones, concentrations of the same order of magnitude as those used in other ecotoxicological studies (Debenest et al, 2009;Roubeix et al, 2011a). The cultures were thus exposed to these four treatments over 7 days, during which samples were taken for chemical and biological measurements.…”

Section: 1gomphonema Gracilementioning

confidence: 99%

Use of diatom motility features as endpoints of metolachlor toxicity

et al. 2015

Self Cite

View full text Add to dashboard Cite

Many recent ecotoxicological studies suggest a relationship between freshwater contamination and increasing abundances of motile diatoms (potentially able to move). The capacity to escape would present advantages to species in polluted environments. However, actual motility as a response to toxicants had not been described and required experimental validation. We designed a specific experiment to assess how a field-isolated diatom (Gomphonema gracile) distributes energy to in situ resistance (increased population growth or photosynthesis) and escape (behavioral changes), when exposed to increasing concentrations of the herbicide metolachlor. We report here the dose-time dependent responses of G. gracile populations. They coped with low contamination by resisting in situ, with early hormetic responses highlighted by stimulation of chlorophyll-a fluorescence. At a higher dose, harmful impacts were observed on growth after a few days, but an earlier behavioral response suggested that higher motility (percentage of motile individuals and mean distance crossed) could be involved in escape. Our findings bring new arguments to support the implementation of real measurements instead of motility traits in toxicity assessment. Specifically, motion descriptors have been used as early-warning indicators of contamination in our study. Further works should address the reliability of these endpoints in more complex conditions (interspecific variability, behavior in the field).

show abstract

Section: 1gomphonema Gracilementioning

confidence: 99%

Use of diatom motility features as endpoints of metolachlor toxicity

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…80% acetonitrile is often required, significantly increasing LODs. In environmental analysis, HILIC was applied for the targeted analysis of polar herbicides [71], pesticides [72], and pharmaceuticals, such as antibiotics [17], drugs of abuse [16], cytostatics [73], antidiabetics [24,74], and contrast agents [75,76]. HILIC was successfully used as a complementary method to RPLC for the non-target screening of emerging polar organic compounds in wastewater [77].…”

Section: Hydrophilic Interaction Liquid Chromatographymentioning

confidence: 99%

Trends in sample preparation and separation methods for the analysis of very polar and ionic compounds in environmental water and biota samples

2020

View full text Add to dashboard Cite

Recent years showed a boost in knowledge about the presence and fate of micropollutants in the environment. Instrumental and methodological developments mainly in liquid chromatography coupled to mass spectrometry hold a large share in this success story. These techniques soon complemented gas chromatography and enabled the analysis of more polar compounds including pesticides but also household chemicals, food additives, and pharmaceuticals often present as traces in surface waters. In parallel, sample preparation techniques evolved to extract and enrich these compounds from biota and water samples. This review article looks at very polar and ionic compounds using the criterion log P ≤ 1. Considering about 240 compounds, we show that (simulated) log D values are often even lower than the corresponding log P values due to ionization of the compounds at our reference pH of 7.4. High polarity and charge are still challenging characteristics in the analysis of micropollutants and these compounds are hardly covered in current monitoring strategies of water samples. The situation is even more challenging in biota analysis given the large number of matrix constituents with similar properties. Currently, a large number of sample preparation and separation approaches are developed to meet the challenges of the analysis of very polar and ionic compounds. In addition to reviewing them, we discuss some trends: for sample preparation, preconcentration and purification efforts by SPE will continue, possibly using upcoming mixed-mode stationary phases and mixed beds in order to increase comprehensiveness in monitoring applications. For biota analysis, miniaturization and parallelization are aspects of future research. For ionic or ionizable compounds, we see electromembrane extraction as a method of choice with a high potential to increase throughput by automation. For separation, predominantly coupled to mass spectrometry, hydrophilic interaction liquid chromatography applications will increase as the polarity range ideally complements reversed phase liquid chromatography, and instrumentation and expertise are available in most laboratories. Two-dimensional applications have not yet reached maturity in liquid-phase separations to be applied in higher throughput. Possibly, the development and commercial availability of mixed-mode stationary phases make 2D applications obsolete in semi-targeted applications. An interesting alternative will enter routine analysis soon: supercritical fluid chromatography demonstrated an impressive analyte coverage but also the possibility to tailor selectivity for targeted approaches. For ionic and ionizable micropollutants, ion chromatography and capillary electrophoresis are amenable but may be used only for specialized applications such as the analysis of halogenated acids when aspects like desalting and preconcentration are solved and the key advantages are fully elaborated by further research.

show abstract

“…The selection of an SPE sorbent that includes both polar and nonpolar moieties was the first requirement for retaining the whole range of target chemicals (1.6 < log Kow < 9.5). Among them, Oasis HLB is identified as the best candidate for extracting a wide range of contaminants (Kaserzon et al, 2012;Fauvelle et al, 2015;Net et al, 2015a). An important SPE step was the selection of an appropriate elution fraction.…”

Section: Spe Optimizationmentioning

confidence: 99%

One-Single Extraction Procedure for the Simultaneous Determination of a Wide Range of Polar and Nonpolar Organic Contaminants in Seawater

et al. 2018

Self Cite

View full text Add to dashboard Cite

A rapid analytical method including one-single solid-phase extraction (SPE) procedure followed by gas and liquid chromatography coupled with high resolution mass spectrometry detection (GC-MS and LC-HRMS respectively) was developed for the quantification of 40 organic compounds (1.6 < log K ow < 9.5) in seawater including both legacy and emerging contaminants, with a focus on the most common plastic organic additives. This new method allowed for the analyses of nine organophosphate esters (OPEs), seven phthalates (PAEs), six bisphenols (BPs), five perfluorinated compounds (PFCs), and thirteen legacy organochlorinated compounds (OCs, including polychlorobiphenyles and pesticides) with recoveries in the ranges of 57-124% for OPEs, 52-163% for PAEs, 64-118% for BPs, 63-124% for PFCs, and 40-95% for OCs. As a result of (i) strict cleanup protocols, (ii) material, and solvent selection, and (iii) the use of an ISO 6 cleanroom for sample treatment, the procedural blank levels were always lower than 5 ng L −1 , even for the most abundant and ubiquitous compounds like tris-(2-chloro, 1-methylethyl) phosphate (TCPP) and diethylhexyl phthalate (DEHP). The quantification limits were in the ranges of 0.03-0.75 ng L −1 for OPEs, 0.03-0.25 ng L −1 for PAEs, 0.1-5 ng L −1 for BPs, 0.1-8 ng L −1 for PFCs, and 0.02-1.1 ng L −1 for OCs, matching seawater analysis requirements. Dissolved water phase samples collected in Marseille Bay (NW Mediterranean Sea) were analyzed using the developed method reveling the concentration of PAEs up to 140 ng L −1 (DEHP) and that of OCs up to 70 ng L −1 (α-endosulfan). For the first time, we also provided the concentrations of OPEs (TCPP up to 450 ng L −1), BPs (bisphenol S up to 123 ng L −1), and PFCs (PFOS up to 5 ng L −1) in this area. A sampling station close to the municipal waste water treatment plant outfall exhibited the highest concentration levels for all compounds.

show abstract

Hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for acidic herbicides and metabolites analysis in fresh water

Cited by 18 publications

References 32 publications

Use of diatom motility features as endpoints of metolachlor toxicity

Use of diatom motility features as endpoints of metolachlor toxicity

Trends in sample preparation and separation methods for the analysis of very polar and ionic compounds in environmental water and biota samples

One-Single Extraction Procedure for the Simultaneous Determination of a Wide Range of Polar and Nonpolar Organic Contaminants in Seawater

Contact Info

Product

Resources

About