Hydrophilic phytic acid-functionalized magnetic dendritic mesoporous silica nanospheres with immobilized Ti4+: A dual-purpose affinity material for highly efficient enrichment of glycopeptides/phosphopeptides

Hong, Yayun; Zhan, Qiliang; Zheng, Yu; Pu, Chenlu; Zhao, Hongli; Lan, Minbo

doi:10.1016/j.talanta.2019.01.005

Cited by 50 publications

(19 citation statements)

References 45 publications

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“…In-syringe-type fast and robust enrichment of phosphopeptides was also made using Ti(IV) attached-phosphonic acid-functionalized capillary monolith obtained by the in-situ copolymerization of vinylphosphonic acid and ethylene dimethacrylate [7]. Magnetic mesoporous silica microspheres were modified with polyethyleneimine (PEI) and phytic acid (PA) by Hong et al [54]. The fact that PA has six phosphate groups that allow the immobilization of large amounts of Ti(IV) as it creates strong affinity sites for binding metal ions.…”

Section: F I G U R E 2 Bioaffinity Chromatography Methods Target Biomentioning

confidence: 99%

“…Today, inorganic materials such as silica, carbon-based materials, magnetic nanoparticles, and metal-organic frameworks (MOFs) are widely used as support materials in the synthesis of IMAC sorbents. Several silica-based sorbents both in the magnetic and non-magnetic forms were successfully tried for the enrichment of phosphorylated proteins [44,47,54,[60][61][62]. Silica-based supports have several advantages such as good mechanical stability, rigid 3D structure, tunable pore size, large surface area, and facile derivatization [63].…”

Section: Techniques Advantages Disadvantagesmentioning

confidence: 99%

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Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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Section: F I G U R E 2 Bioaffinity Chromatography Methods Target Biomentioning

confidence: 99%

Section: Techniques Advantages Disadvantagesmentioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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“…Recently, Hong et al [169] prepared Mag-MSMs with ordered radial mesochannels by a self-assembly synthesis in the chlorobenzeneÀ water mixed system. The obtained the Mag-MSM was then modified by layer-by-layer assembly with polyethyleneimine (PEI), phytic acid (PA) and Ti 4 + (denoted as Mag-MSMs@PEIÀ PAÀ Ti 4 + ).…”

Section: Immobilized Metal Ions For the Enrichment Of Phosphopeptidesmentioning

confidence: 99%

“…Recently, Hong et al . prepared Mag‐MSMs with ordered radial mesochannels by a self‐assembly synthesis in the chlorobenzene−water mixed system.…”

Section: Applications For Core‐shell Mag‐msnsmentioning

confidence: 99%

Recent Advances in the Synthesis, Surface Modifications and Applications of Core‐Shell Magnetic Mesoporous Silica Nanospheres

Zuo

et al. 2020

Chemistry An Asian Journal

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The hierarchically structured core-shell magnetic mesoporous silica nanospheres (Mag-MSNs) have attracted extensive attention, particularly in studies involving reliable preparations and diverse applications of the multifunctional nanomaterials in multi-disciplinary fields. Intriguingly, Mag-MSNs have been prepared with well-designed synthesis strategies and used as adsorbent materials, biomedicines, and in proteomics and catalysis due to their excellent magnetic responsiveness, enormous specific surface area and readiness for surface modifications. Through a carefully designed surface modification of Mag-MSNs, the performance and application prospects of the material are greatly improved. Typically, the introduction of various molecular matrices into the shell of Mag-MSNs facilitates the combination of surface modifications and magnetic separation technology. So far, as sustainable chemistry is concerned, it is important to recover the functionalized core-shell Mag-MSNs after the reaction and reuse them without losing activity. In this review, the design conceptions and the construction of core-shell Mag-MSNs are discussed. Furthermore, various surface modification approaches of core-shell Mag-MSNs are summarized, and recent applications of these functionalized nanomaterials in the fields of biomedicine, catalysis, proteomics and wastewater treatment are exemplified. 2 3 4 5 6 7 8

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“…Aberrant phosphorylation is associated with human diseases, such as Alzheimer's disease, 7 cardiovascular disease, and cancer 8 . Related research shows that aberrant phosphoproteins with high clinical specificity and sensitivity have become the biomarkers of clinical diagnosis, which means that research into aberrant phosphoproteins will help study the pathogenesis of human diseases 9 . However, when the biological sample is directly measured by mass spectrometry (MS), 10 the low ionization efficiency of phosphopeptides and the signal suppression of non‐phosphopeptides seriously affect the detection of the phosphopeptides 11 .…”

Section: Introductionmentioning

confidence: 99%

In situ synthesis of a novel metal oxide affinity chromatography affinity probe for the selective enrichment of low‐abundance phosphopeptides

Wang

Yan

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Due to the dynamic nature of phosphorylation states and the low stoichiometry of phosphopeptides, it is still a challenge to efficiently capture phosphopeptides from complex biological samples before mass spectrometry analysis. Among the enrichment strategies, metal oxide affinity chromatography (MOAC) is one of the most widely used and the one with the most potential. It is based on reversible Lewis acid–base interactions between the metal oxides and the negatively charged phosphate groups to achieve the specific selection of phosphopeptides. Methods A novel MOAC affinity probe, denoted as G@PDA@ZrO2, was successfully synthesized by in situ grafting ZrO2 onto the surface of graphene (G) modified with polydopamine (PDA). The novel MOAC probe thus obtained was used for phosphopeptide enrichment. Results This novel MOAC affinity probe when used to selectively enrich phosphopeptides from standard protein digest solutions exhibited a high selectivity (β‐casein:bovine serum albumin = 1:1000), a low detection limit (4 fmol), and a high loading capacity (400 mg/g). At the same time, the experimental results proved that G@PDA@ZrO2 had great recyclability (five cycles), stability, and reproducibility. Subsequently, G@PDA@ZrO2 was applied to enrich phosphopeptides from human saliva and human serum, in which 25 and 4 phosphopeptide peaks, respectively, were detected. Conclusions This novel MOAC affinity probe (G@PDA@ZrO2) showed good performance in enriching phosphopeptides. Thus, G@PDA@ZrO2 has good potential in phosphopeptidomics analysis.

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Hydrophilic phytic acid-functionalized magnetic dendritic mesoporous silica nanospheres with immobilized Ti4+: A dual-purpose affinity material for highly efficient enrichment of glycopeptides/phosphopeptides

Cited by 50 publications

References 45 publications

Recent trends in sorbents for bioaffinity chromatography

Recent trends in sorbents for bioaffinity chromatography

Recent Advances in the Synthesis, Surface Modifications and Applications of Core‐Shell Magnetic Mesoporous Silica Nanospheres

In situ synthesis of a novel metal oxide affinity chromatography affinity probe for the selective enrichment of low‐abundance phosphopeptides

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