Hydrophobic Interaction‐Mediated Capture and Release of Cancer Cells on Thermoresponsive Nanostructured Surfaces

Liu, Hongliang; Liu, Xueli; Meng, Jing; Zhang, Pengchao; Gao, Yang; Su, Bin; Sun, Kang; Chen, Li; Han, Dong; Wang, Shutao; Jiang, Lei

doi:10.1002/adma.201203826

Cited by 250 publications

(204 citation statements)

References 37 publications

Supporting

Mentioning

198

Contrasting

Unclassified

Order By: Relevance

“…Captured cells were then stained with PE-labeled anti-CK, FITC-labeled anti-CD45 and DAPI. [26][27][28][29] Fluorescence microscopy was used to distinguish specifically captured CTCs (CK+/CD45-/DAPI+) from non-specifically immobilized WBCs (CK-/CD45+/DAPI+) by their fluorescence and larger size (CTCs: 10-30 µm; 1 WBCs, 15 µm) ( Figure 6A). CTC enumeration results are summarized in Figure 6B and Table 1.…”

Section: Sa-anti-epcam-flat Glass S a -A N T I -E P C A M F L A T G Lmentioning

confidence: 99%

Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

Cheng¹,

He²,

Zhao³

et al. 2014

IJN

View full text Add to dashboard Cite

Circulating tumor cells (CTCs) in the blood which have detached from both the primary tumor and any metastases may be considered as a “liquid biopsy” and are expected to replace tumor biopsies in the monitoring of treatment response and determining patient prognosis. Here, we introduce a facile and efficient CTC detection material made of hydroxyapatite/chitosan (HA/CTS), which is beneficial because of its transparency and excellent biological compatibility. Atomic force microscopy images show that the roughness of the HA/CTS nanofilm (HA/CTSNF) substrates can be controlled by changing the HA:CTS ratio. Enhanced local topographic interactions between nano-components on cancer cell membranes, and the antibody coated nanostructured substrate lead to improved CTC capture and separation. This remarkable nanostructured substrate has the potential for CTC culture in situ and merits further analysis. CTCs captured from artificial blood samples were observed in culture on HA/CTSNF substrates over a period of 14 days by using conventional staining methods (hematoxylin eosin and Wright’s stain). We conclude that these substrates are multifunctional materials capable of isolating and culturing CTCs for subsequent studies.

show abstract

Section: Sa-anti-epcam-flat Glass S a -A N T I -E P C A M F L A T G Lmentioning

confidence: 99%

Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

Cheng¹,

He²,

Zhao³

et al. 2014

IJN

View full text Add to dashboard Cite

show abstract

“…The combination of responsive polymer brushes with 3D nanostructures constitutes a promising candidate for the engineering of advanced materials for various applications, especially in the field of biotechnology [25,26]. For instance, the realization of stimulated and controlled uptake and release of biomolecules into and from the free space in between the nanostructures is expected by the use of polymer brushes ( Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Combined QCM-D/GE as a tool to characterize stimuli-responsive swelling of and protein adsorption on polymer brushes grafted onto 3D-nanostructures

et al. 2014

View full text Add to dashboard Cite

A combined setup of quartz crystal microbalance and generalized ellipsometry can be used to comprehensively investigate complex functional coatings comprising stimuli-responsive polymer brushes and 3D nanostructures in a dynamic, noninvasive in situ measurement. While the quartz crystal microbalance detects the overall change in areal mass, for instance, during a swelling or adsorption process, the generalized ellipsometry data can be evaluated in terms of a layered model to distinguish between processes occurring within the intercolumnar space or on top of the anisotropic nanocolumns. Silicon films with anisotropic nanocolumnar morphology were prepared by the glancing angle deposition technique and further functionalized by grafting of poly-(acrylic acid) or poly-(N-isopropylacrylamide) chains. Investigations of the thermoresponsive swelling of the poly-(N-isopropylacrylamide) brush on the Si nanocolumns proved the successful preparation of a stimuli-responsive coating. Furthermore, the potential of these novel coatings in the field of biotechnology was explored by investigation of the adsorption of the model protein bovine serum albumin. Adsorption, retention, and desorption triggered by a change in the pH value is observed using poly-(acrylic acid) functionalized nanostructures, although generalized ellipsometry data revealed that this process occurs only on top of the nanostructures. Poly-(N-isopropylacrylamide) is found to render the nanostructures non-fouling properties.

show abstract

Section: Anti-biofoulingmentioning

confidence: 99%

Smart Polymers with Special Wettability

Chang

Zhang

Sun

2016

Small

View full text Add to dashboard Cite

Surface wettability plays a key role in addressing issues ranging from basic life activities to our daily life, and thus being able to control it is an attractive goal. Learning from nature, both of its structure and function, brings us much inspiration in designing smart polymers to tackle this major challenge. Life functions particularly depend on biomolecular recognition-induced interfacial properties from the aqueous phase onto either "soft" cell and tissue or "hard" inorganic bone and tooth surfaces. The driving force is noncovalent weak interactions rather than strong covalent combinations. An overview is provided of the weak interactions that perform vital actions in mediating biological processes, which serve as a basis for elaborating multi-component polymers with special wettabilities. The role of smart polymers from molecular recognitions to macroscopic properties are highlighted. The rationale is that highly selective weak interactions are capable of creating a dynamic synergetic communication in the building components of polymers. Biomolecules could selectively induce conformational transitions of polymer chains, and then drive a switching of physicochemical properties, e.g., roughness, stiffness and compositions, which are an integrated embodiment of macroscopic surface wettabilities.

show abstract

Hydrophobic Interaction‐Mediated Capture and Release of Cancer Cells on Thermoresponsive Nanostructured Surfaces

Cited by 250 publications

References 37 publications

Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

Transparent, biocompatible nanostructured surfaces for cancer cell capture and culture

Combined QCM-D/GE as a tool to characterize stimuli-responsive swelling of and protein adsorption on polymer brushes grafted onto 3D-nanostructures

Smart Polymers with Special Wettability

Contact Info

Product

Resources

About