Hydrophobic ionic liquid immoblizing cholesterol oxidase on the electrodeposited Prussian blue on glassy carbon electrode for detection of cholesterol

Recent advances in ionic liquid-based electrochemical biosensors

“…Attributed to the high viscosity, ILs can be used as the binder on the electrode, and may effectively immobilize the sensitive biomolecules onto the surface of the electrode. While the influence of the viscosity on the rate of mass transport in the solutions should be taken into account [11]. The most attractive advantages for their applications in electrochemical sensors are their excellent electrochemical properties including both the wide electrochemical window [12] and the good electrical conductivity [13].…”

“…For the fabrication of the electrochemical biosensors, the biocompatibility of ILs and their effect on the natural activity of biomolecules such as the enzymes should be seriously considered. Satisfyingly, ILs exhibit good biocompatibility with biomolecules [11,[16][17][18][19][20][21][22][23]. ILs could also enhance the activity of some biomolecules, leading to the more sensitive and more satisfactory detection results.…”

Bimetallic Co2+ and Mn2+ Hexacyanoferrate for Hydrogen Peroxide Electrooxidation and Its Application in a Highly Sensitive Cholesterol Biosensor

“…The determination of cholesterol is routinely performed spectrophotometrically, [3][4][5][6] but the implementation of electrochemical cholesterol biosen-sors is of paramount importance to advance towards device portability with the added advantage of real-time monitoring and suitability for miniaturization. [7,8] Cholesterol oxidase is capable of oxidizing cholesterol and producing hydrogen peroxide at the same time. [7,8] Cholesterol oxidase is capable of oxidizing cholesterol and producing hydrogen peroxide at the same time.…”

“…Commonly, cholesterol biosensing is performed with the aid of cholesterol oxidase immobilized on a suitable electrode. [7,8] Cholesterol oxidase is capable of oxidizing cholesterol and producing hydrogen peroxide at the same time. Both the recognition event and biochemical oxidation of cholesterol have to be transduced into an electrical signal, which is achieved by including an adequate hydrogen peroxide electrocatalyst at the interface.…”

Synthesis of Surface‐Modification‐Oriented Nanosized Molybdenum Disulfide with High Peroxidase‐Like Catalytic Activity for H2O2 and Cholesterol Detection

“…The ab-normalH 2 O 2 level in tissues can potentially induce various types of damage to cellular compartments, consequently causing aging, atherosclerosis, cancer,a nd renal diseases. Therefore, it is extremelye ssential to establish reliable methods to detectc holesterol with high sensitivitya nd selectivity.T od ate, various methods such as electrochemistry sensors, [6][7][8][9][10][11][12] fluorometric analysis, [13][14][15] chromatographic analysis, [16][17][18] molecular imprinting techniques, [19] surface plasma resonance, [20] fieldeffect-transistor sensors, [21] chemiluminescence, [22] and colorimetric methods [23,24] have been reported to detect cholesterol and H 2 O 2 .A mongt hem, the colorimetric methodi sp opular becauseo fi ts rapid ands imple operation as well as good reproducibility.I na ddition, the color change during colorimetric detection could be directly observed by the eyes insteado fb y using expensive instruments. [25,26] It is well known that natural horseradish peroxidase (HRP) can be used for colorimetricd etection.…”