Hydrophobin HGFI–based fibre-optic biosensor for detection of antigen–antibody interaction

Abstract

A compact S-tapered fibre biosensor functionalised with class I hydrophobin HGFI found in Grifola frondosa has been proposed for the first time to accrue label-free detection of reaction between goat–anti-rabbit immunoglobulin G (IgG) (GAR, antibody) and rabbit–anti-hemagglutinin IgG (R, antigen). The HGFI nanolayer self-assembled on fibre surface provides a distinguished analytical platform to implement biocompatible binding owing to its prominent amphiphilicity and remarkable optical as well as biochemical properties. Water contact angle measurements, atomic force microscopy, and immunofluorescence assay are utilised to characterize the modification properties of siliconised substrate surface with HGFI. Functionalisation of the S-tapered fibre is achieved by exploiting self-assembly of HGFI and immobilisation of GAR for specific R detection. HGFI with a concentration of 300 μg/ml allows the formation of a self-assembled amphipathic film on fibre surface, which can adsorb antibody smoothly with such desirable merits as ease of operation, fast response, good stability, good repeatability, and no side effects. Immunoassay experiments are conducted based on dip wavelengths interrogation of the interferometric transmission spectrum. In this study, we propose a novel antigen detection scheme, which could be extended for the detection of other biomolecules owing to its high integration, good specificity, real-time detection, and simple detection scheme.

Impact of temperature on optical sensing in biology based on investigation of SARS‐CoV‐2

“…Presented graph confirms proper operation of the sensor. Moreover, it is known form the literature that the immobilized proteins are stable in the surface of optical fiber and the antigens can be presented for antibody interaction [ 26 , 27 , 28 ].…”

Innovative surface bio-functionalization by fungal hydrophobins and their engineered variants

“…Functionalization of optical fibers was achieved by exploiting the self-assembly of HPB and the immobilization of goat-anti-rabbit IgG for the development of a label-free immunosensor. HGFI allows the formation of a self-assembled amphipathic film on the fiber surface, which can adsorb antibodies with desirable advantages such as ease of use, quick response, good stability, good repeatability, good specificity, and no side effects ( Duan et al, 2020 ). Following a different approach, the class II HPB HFBI was fused to Protein A, a protein known to bind different immunoglobulin subclasses, and the HFBI–Protein A monolayer was proven to bind to immunoglobin; thus, a highly sensitive label-free graphene biosensor was built ( Soikkeli et al, 2016 ).…”

Applications of Hydrophobins in Medical Biotechnolog

“…Designing Biointerfaces (Antibacterial interfaces) (Wang, et al, 2016) Detection of Antibody (Duan, et al, 2020) Vmh2 Pleurotus ostreatus PC15 Development of Antimicrobial Surfaces using a Hydrophobin Chimeric (Sorrentino, et al, 2020) Immobilization of Antibodies ( Stanzione, et al, 2021)…”