Hydrostatic Pressure Improves Maturation of Oocyte in Cultures of Follicles Derived from Mouse Polycystic Ovary

Kalhori, Zahra

doi:10.4183/aeb.2015.26

Cited by 2 publications

(1 citation statement)

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“…To induce polycystic ovary, mice were injected daily with testosterone enanthate 1 mg/100g body weight dissolved in sesame oil (PCO group), while non-PCO group were injected only with vehicle for 4 weeks as described in our previous study (22).…”

Section: Polycystic Ovary Inductionmentioning

confidence: 99%

Effect of Progesterone on Survival, in vitro Growth and in Vitro Maturation of Follicle Derived from Mouse Polycystic Ovary

Bazdar

Azadbakht²

2018

Acta Endo (Buc)

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Context. Polycystic ovarian syndrome is the most common endocrine disorder in women of reproductive age. Objective. The purpose of the present study is to investigate the role of progesterone on survival, in vitro growth and in vitro maturation of follicles and the maturation of oocytes derived from mouse polycystic ovary. Subjects and Methods. To induce polycystic ovary (PCO) female NMRI mice 21 days old were injected daily with testosterone enanthate 1 mg/100g body weight dissolved in sesame oil for 4 weeks (PCO group), while non-PCO group were injected only with vehicle. Follicles were derived from both groups and cultured in MEM-α medium either with progesterone or without progesterone. Sizes of follicles were measured in days 1, 3, 6, 9 and 12. After 12 days follicles were transferred to mature medium, follicles from two groups were cultured for 24 and 48 h and the in vitro maturation of oocytes was assessed. Results. In PCO groups with progesterone, survival and in vitro growth of follicles, significantly increased as compared with PCO groups without progesterone (p<0.05). The in vitro maturation rate in PCO group with progesterone was significantly higher than in those not treated by progesterone (p<0.05). Conclusions. This study demonstrated that progesterone can improve survival, in vitro growth and in vitro maturation of follicles derived from mouse polycystic ovary.

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Section: Polycystic Ovary Inductionmentioning

confidence: 99%

Effect of Progesterone on Survival, in vitro Growth and in Vitro Maturation of Follicle Derived from Mouse Polycystic Ovary

Bazdar

Azadbakht²

2018

Acta Endo (Buc)

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L-Carnitine improves endocrine function and folliculogenesis by reducing inflammation, oxidative stress and apoptosis in mice following induction of polycystic ovary syndrome

Kalhori

Azadbakht

Shariatzadeh

2019

Reprod. Fertil. Dev.

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Polycystic ovary syndrome (PCOS) is related to low levels of serum l-carnitine, which has antioxidant, anti-inflammatory and antiapoptotic properties. The aim of this study was to investigate the effect of l-carnitine on folliculogenesis in mice following induction of PCOS. PCOS was induced by daily injections of testosterone enanthate (1mg per 100g, s.c., for 35 days). NMRI mice (21 days old) were divided into four groups (n=6 per group): Control, Control+l-carnitine, PCOS and PCOS+l-carnitine. Mice were treated with 500mgkg−1, i.p., l-carnitine every second day for 28 days. Ovaries were studied stereologically and serum concentrations of FSH, LH, testosterone, interleukin (IL)-6 and tumour necrosis factor (TNF)-α were determined using ELISA kits. Serum concentrations of malondialdehyde (MDA) and the ferric ion reducing antioxidant power (FRAP) were also analysed. Apoptosis of follicles was evaluated by terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling (TUNEL). CD31 was assessed immunohistochemically. Data were analysed using one-way analysis of variance (ANOVA) and Tukey’s test, differences considered significant at P<0.05.The total volume of the ovary, cortex volume, oocyte volume, zona pellucida thickness and the number of antral follicles increased significantly, whereas the number of primary and preantral follicles decreased significantly, in the PCOS+l-carnitine versus PCOS group. In the PCOS+l-carnitine group, serum concentrations of FSH and FRAP increased significantly, whereas there were significant decreases in serum concentrations of testosterone, LH, MDA, IL-6 and TNF-α, as well as in the percentage of TUNEL-positive apoptotic cells, compared with the PCOS group. l-Carnitine improves folliculogenesis and is therefore suggested as a therapeutic supplement in the treatment of PCOS.

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Hydrostatic Pressure Improves Maturation of Oocyte in Cultures of Follicles Derived from Mouse Polycystic Ovary

Cited by 2 publications

References 0 publications

Effect of Progesterone on Survival, in vitro Growth and in Vitro Maturation of Follicle Derived from Mouse Polycystic Ovary

Effect of Progesterone on Survival, in vitro Growth and in Vitro Maturation of Follicle Derived from Mouse Polycystic Ovary

L-Carnitine improves endocrine function and folliculogenesis by reducing inflammation, oxidative stress and apoptosis in mice following induction of polycystic ovary syndrome

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