Hydroxyapatite coating of cellulose sponge does not improve its osteogenic potency in rat bone

Ekholm, Erika; Tommila, Miretta; Forsback, Ari-Pekka; Märtson, Matis; Holmbom, Johanna; Ääritalo, Virpi; Finnberg, Christa; Kuusilehto, Asko; Salonen, Jukka; Yli‐Urpo, Antti; Penttinen, Risto

doi:10.1016/j.actbio.2005.05.003

Cited by 35 publications

(22 citation statements)

References 27 publications

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“…The effects of surface modification with calcium phosphate have received mixed reviews in the literature. Some research groups show positive effects of these coatings on bone regeneration and the mechanical properties of the coated biomaterials , whereas others show no beneficial effects of the coating with respect to bone regeneration (Ekholm et al 2005;Fontana et al 2011). It should be noted, however, that most of the defects treated in the aforementioned studies were small bone defects (not critical-sized, thereby able to heal unassisted) or were studies to demonstrate the effects of surface modification on implant ingrowth (Fontana et al 2011;Mihalko et al 2010).…”

Section: Discussionmentioning

confidence: 94%

Biomimetic tubular nanofiber mesh and platelet rich plasma-mediated delivery of BMP-7 for large bone defect regeneration

et al. 2012

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There is a growing need for successful bone tissue engineering strategies and advanced biomaterials that mimic the structure and function of native tissues carry great promise. Successful bone repair approaches may include an osteoconductive scaffold, osteoinductive growth factors, cells with an osteogenic potential and capacity for graft vascularisation. To increase osteoinductivity of biomaterials, the local combination and delivery of growth factors has been developed. In the present study we investigated the osteogenic effects of calcium phosphate (CaP)-coated nanofiber mesh tube-mediated delivery of BMP-7 from a PRP matrix for the regeneration of critical sized segmental bone defects in a small animal model. Bilateral full-thickness diaphyseal segmental defects were created in twelve male Lewis rats and nanofiber mesh tubes were placed around the defect. Defects received either treatment with a CaP-coated nanofiber mesh tube (n = 6), an un-coated nanofiber mesh tube (n=6) a CaP-coated nanofiber mesh tube with PRP (n=6) or a CaP-coated nanofiber mesh tube in combination with 5 μg BMP-7 and PRP (n = 6). After 12 weeks, bone volume and biomechanical properties were evaluated using radiography, microCT, biomechanical testing and histology. The results demonstrated significantly higher biomechanical properties and bone volume for the BMP group compared to the control groups. These results were supported by the histological evaluations, where BMP group showed the highest rate of bone regeneration within the defect. In conclusion, BMP-7 delivery via PRP enhanced functional bone defect regeneration, and together these data support the use of BMP-7 in the treatment of critical sized defects.

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Section: Discussionmentioning

confidence: 94%

Biomimetic tubular nanofiber mesh and platelet rich plasma-mediated delivery of BMP-7 for large bone defect regeneration

et al. 2012

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“…Therefore, CaR seems to have a function of retaining HSCs in close physical nearness to the endosteal surface (Adams et al 2006). The HA coating used in this study resembles the HA of bone (Ekholm et al 2005). Plentiful giant cells/ macrophages are gathered around the mineralized cellulose ( Tommila et al 2008).…”

Section: J R Soc Interfacementioning

confidence: 99%

“…HA-coated cellulose was prepared as described previously (Ekholm et al 2005). In short, simulated body fluids (SBF) with ion concentration close to 1 and 1.5 times that of human blood plasma were prepared, buffered to pH 7.4 and sterilized by filtering.…”

Section: Treatment Of Cellulose Sponges With Bioactive Glassmentioning

confidence: 99%

“…In the bone marrow, the HSC niche is located close to the endosteal surface and is regulated via calciumsensing receptors (CaRs) of HSCs (Adams et al 2006;Drüeke 2006). The HA coating resembles the natural mineral composition of bone (Ekholm et al 2005) and might therefore attract bone-marrow-derived stem cells. Thus, we wanted to investigate whether the granulation tissue-stimulating ability of the HA-coated cellulose implant is due to increased homing of bonemarrow-derived stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous work, we showed that hydroxyapatite (HA) coating of cellulose sponges induces a more effective formation of a highly vascularized granulation tissue than uncoated cellulose when the sponges were implanted subcutaneously in rat (Tommila et al 2008). The HA coating was formed by treating cellulose with a bioactive glass, which creates a silica-rich calcium phosphate layer (Ekholm et al 2005). In the bone marrow, the HSC niche is located close to the endosteal surface and is regulated via calciumsensing receptors (CaRs) of HSCs (Adams et al 2006;Drüeke 2006).…”

Section: Introductionmentioning

confidence: 99%

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Hydroxyapatite coating of cellulose sponges attracts bone-marrow-derived stem cells in rat subcutaneous tissue

Tommila

Jokilammi

Terho

et al. 2009

J. R. Soc. Interface.

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The presence of bone-marrow-derived stem cells was investigated in a wound-healing model where subcutaneously implanted cellulose sponges were used to induce granulation tissue formation. When cellulose was coated with hydroxyapatite (HA), the sponges attracted circulating haemopoietic and mesenchymal progenitor cells more efficiently than uncoated cellulose. We hypothesized that the giant cells/macrophages of HA-coated sponges recognize HA as foreign material, phagocyte or hydrolyse it and release calcium ions, which are recognized by the calcium-sensing receptors (CaRs) expressed on many cells including haemopoietic progenitors. Our results showed, indeed, that the HA-coated sponges contained more CaR-positive cells than untreated sponges. The stem cells are, most probably, responsible for the richly vascularized granulation tissue formed in HA-coated sponges. This cell-guiding property of HA-coated cellulose might be useful in clinical situations involving impaired wound repair.

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Bone responses to rough titanium implants coated with biomimetic Ca‐P in rabbit tibia

Yang

Wang

et al. 2009

J Biomed Mater Res

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A new technique to biomimetic deposition calcium phosphate (BDCaP) coating onto rough titanium substrates has been developed recently. This biomimetic deposition technique seems to be promising. It appears to have some advantages such as an inexpensive and simple set-up, and the possibility to synthesize layers with a defined surface morphology. The aim of this study was to examine the bone responses to BDCaP-coated implants in a rabbit model. Thirty one implants (16 BDCaP and 15 rough) were inserted into both tibia of 15 rabbits. After 2, 4, and 8 weeks following the implantation, the tibias were retrieved and prepared for histological evaluation. After 2 weeks, BDCaP-coated implant showed more bone ingrowth inside threads than the rough implants in medullary region (31.43% vs. 24.38%). Histological and quantitative histomorphometrical measurements demonstrated no more bone ingrowth and bone-implant contact for coated implant as compared with uncoated implant in cortical region at all experiment periods. From the histological viewpoint, the BDCaP coating did not have any positive effect on new bone formation.

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Hydroxyapatite coating of cellulose sponge does not improve its osteogenic potency in rat bone

Cited by 35 publications

References 27 publications

Biomimetic tubular nanofiber mesh and platelet rich plasma-mediated delivery of BMP-7 for large bone defect regeneration

Biomimetic tubular nanofiber mesh and platelet rich plasma-mediated delivery of BMP-7 for large bone defect regeneration

Hydroxyapatite coating of cellulose sponges attracts bone-marrow-derived stem cells in rat subcutaneous tissue

Bone responses to rough titanium implants coated with biomimetic Ca‐P in rabbit tibia

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