Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo

Abstract

Hydroxyapatite nanoparticles (nano-HAPs) have been reported to exhibit antitumor effects on various human cancers, but the effects of nano-HAPs on human glioma cells remain unclear. The aim of this study was to explore the inhibitory effect of nano-HAPs on the growth of human glioma U251 and SHG44 cells in vitro and in vivo. Nano-HAPs could inhibit the growth of U251 and SHG44 cells in a dose-and time-dependent manner, according to methyl thiazoletetrazolium assay and flow cytometry. Treated with 120 mg/L and 240 mg/L nano-HAPs for 48 hours, typical apoptotic morphological changes were noted under Hoechst staining and transmission electron microscopy. The tumor growth of cells was inhibited after the injection in vivo, and the related side effects significantly decreased in the nano-HAP-and-drug combination group. Because of the function of nano-HAPs, the expression of c-Met, SATB1, Ki-67, and bcl-2 protein decreased, and the expression of SLC22A18 and caspase-3 protein decreased noticeably. The findings indicate that nano-HAPs have an evident inhibitory action and induce apoptosis of human glioma cells in vitro and in vivo. In a drug combination, they can significantly reduce the adverse reaction related to the chemotherapeutic drug 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU).

Multifunctional and hybrid systems based on calcium-phosphate and hydroxyapatite for preventive and regenerative medicine

“…Razvojem nanotehnologije, pokazano je da nHAp-a inhibiraju proliferaciju ćelija različitih vrsta tumora, ali njihov uticaj na ljudske ćelije glioma nije dovoljno istražen. U cilju postavljanja teorijskih osnova za kliničku upotrebu ispitivan je inhibitorni efekat nHAp-a na rast ljudskih ćelija glioma (U251 i SHG44), pri čemu je pokazano da nHAp indukuje apoptozu tumorskih ćelija glioma in vitro i in vivo, a u kombinaciji sa hemoterapeutskim medikamentom: BCNU (1,3-bis(2-chloroethyl)-1-nitrosourea) značajno smanjuje nuspojave [74].…”

The Ins and Outs of Nanoparticle Technology in Neurodegenerative Diseases and Cancer

“…nHA with the size of >100nm has been developed over the past years. Several groups have shown that nHA NPs have minimal effects on healthy cells whilst at the same time inhibit specifically the cellular proliferation of cancer cells, such as liver and glioma cells [52][53][54]. nHA NPs coated with a fluorescent dye are great carriers for applications such as imaging or for photodynamic therapy.…”

Nonlinear Response Amplification Mechanisms for Low Doses of Natural Product Nanomedicines: Dynamical Interactions with the Recipient Complex Adaptive System

“…The nanoparticle form of calcium phosphate has antiproliferative and pro-apoptotic effects on glioma [176] and osteosarcoma [54] cells. Similarly, their homeopathically-manufactured counterparts, e.g., Ruta graveolens 6C and Calcarea Phosphorica 3X [172], are part of a leading combination anti-cancer protocol for gliomas [108,172].…”

“…The diagnosisbased selection of medicines facilitates providing care from a public health perspective to a large number of patients. Bulk herb extracts of Ruta graveolens, for instance, exert anti-cancer effects [173,174]; and an oral nanoscale formulation of one of its main constituents, rutin, has good bioavailability [175].The nanoparticle form of calcium phosphate has antiproliferative and pro-apoptotic effects on glioma [176] and osteosarcoma [54] cells. Similarly, their homeopathically-manufactured counterparts, e.g., Ruta graveolens 6C and Calcarea Phosphorica 3X [172], are part of a leading combination anti-cancer protocol for gliomas [108,172].…”