Hydroxyl radicals generated by hydrogen peroxide photolysis recondition biofilm-contaminated titanium surfaces for subsequent osteoblastic cell proliferation

Abstract

Titanium dental implants have been successfully used for decades; however, some implants are affected by peri-implantitis due to bacterial infection, resulting in loss of supporting bone. This study aimed to evaluate the effect of an antimicrobial chemotherapy employing H2O2 photolysis—developed to treat peri-implantitis—on biofilm-contaminated titanium surfaces in association with osteoblastic cell proliferation on the treated surface. Titanium discs were sandblasted and acid-etched, followed by contamination with a three-species biofilm composed of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mitis. This biofilm model was used as a simplified model of clinical peri-implantitis biofilm. The discs were subjected to ultrasound scaling, followed by H2O2 photolysis, wherein 365-nm LED irradiation of the disc immersed in 3% H2O2 was performed for 5 min. We analysed proliferation of mouse osteoblastic cells (MC3T3-E1) cultured on the treated discs. Compared with intact discs, biofilm contamination lowered cell proliferation on the specimen surface, whereas H2O2 photolysis recovered cell proliferation. Thus, H2O2 photolysis can recover the degraded biocompatibility of biofilm-contaminated titanium surfaces and can potentially be utilised for peri-implantitis treatment. However, to verify the findings of this study in relation to clinical settings, assessment using a more clinically relevant multi-species biofilm model is necessary.

Influence of aerosol injection on the liquid chemistry induced by an RF argon plasma jet

“…Furthermore, the photo-dissociation of long-lived species (or their precursors, e.g. • NO 2 ) occurring by increasing the treatment time could contribute to their decomposition in liquid both for Ncontaining species [61,62], and for hydrogen peroxide [61,[63][64][65][66], mainly due to Ar excimers radiation (λ max = 126 nm) as shown in figure 4. To support this hypothesis, the high amount of species from water photolysis in long distances with plasma-only, shown in figure 7.…”

Protective Role of Sphingomyelin in Eye Lens Cell Membrane Model against Oxidative Stress

“…Therefore, they provide a powerful platform to study the membrane properties and interaction of reactive species with lipid bilayer [ 34 , 35 , 36 ]. Various methods have been developed for generating reactive species, such as Fenton reaction [ 37 , 38 ], photolysis [ 39 , 40 ] and cold physical plasma (CPP) [ 41 , 42 ]. CPP as ionized gas with temperature close to human body temperature can be produced by different sources such as plasma jets or dielectric barrier discharges [ 42 , 43 ], and beneficial effects have been shown in several medical applications, including wound healing [ 44 , 45 ], cancer treatment [ 46 , 47 , 48 , 49 ] and dental care [ 50 ].…”

Inhibition of tooth demineralization caused by Streptococcus mutans biofilm via antimicrobial treatment using hydrogen peroxide photolysis

“…Half of the samples in each group ( n = 6) were subjected to determination of viable bacterial counts immediately after treatment, while the remaining samples were additionally cultured in BHI-S for 30 h under the same conditions as described above, followed by the determination of viable bacterial counts. For viable counts, the biofilms were detached from the rat molar using an enzymatic technique according to previous studies [ 30 , 31 ]. The samples were immersed in 500 μL of an enzyme solution, composed of 4 mg/mL type I collagenase (Thermo Fisher Scientific, Waltham, MA, USA) and 2 mg/mL dispase (Thermo Fisher Scientific) in phosphate-buffered saline.…”