Hydroxyl scavenging activity accounts for differential antioxidant protection of <i>Plantago major</i> against oxidative toxicity in isolated rat liver mitochondria

Mello, Joyce C.; Guimarães, Natalia S.S.; Gonzalez, Mariano V D; Paiva, Juliana S; Prieto, Tatiana; Nascimento, Otaciro Rangel; Rodrigues, Tiago

doi:10.1111/j.2042-7158.2012.01496.x

Cited by 14 publications

(12 citation statements)

References 41 publications

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“…Although widely used in popular medicine, extraction using ethanolic solution has been poorly studied in the Plantago genus as methanol is ordinarily used. The amount of phenols in PmHE were lower than those observed in methanolic extracts of P. major , whose reported concentration was 9.17% [26], or methanolic extracts of other genus, such as P. lagopus that presented 7.99% [27].…”

Section: Resultsmentioning

confidence: 99%

Protective Effect of Plantago major Extract against t-BOOH-Induced Mitochondrial Oxidative Damage and Cytotoxicity

et al. 2015

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Plantago major L. produces several chemical substances with anti-inflammatory and analgesic activities and its use in the treatment of oral and throat inflammation in popular medicine is well described. In this study, the antioxidant potential of the Plantago major hydroethanolic extract was screened and its protective action was evaluated against t-BOOHinduced oxidative stress. The extract was obtained by fractionated percolation using 50% ethanolic solution and, after drying, suspended in dimethyl sulfoxide. The chromatographic profile of crude extract was obtained with the identification of some phytochemical markers and the total phenols and flavonoids were quantified. The scavenger activity against DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals was determined and the antioxidant activity in biological systems was evaluated in isolated rat liver mitochondria and HepG2 cells. The extract exhibited a significant free radical scavenger activity at 0.1 mg/mL, and decreased the ROS (reactive oxygen species) generation in succinate-energized mitochondria. Such an effect was associated with the preservation of the intrinsic antioxidant defenses (reduced glutathione OPEN ACCESSMolecules 2015, 20 17748 and NAD(P)H) against the oxidation by t-BOOH, and also to the protection of membranes from lipid oxidation. The cytoprotective effect of PmHE against t-BOOH induced cell death was also shown. These findings contribute to the understanding of the health benefits attributed to P. major.

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Section: Resultsmentioning

confidence: 99%

Protective Effect of Plantago major Extract against t-BOOH-Induced Mitochondrial Oxidative Damage and Cytotoxicity

et al. 2015

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“…Ethyl acetate and ethyl:methanol (10:1) extract showed a similar shape of absorbance pattern. Similarly, in another study aimed at characterizing the different polarity extracts obtained from Plantago major, methanol and ethyl acetate extracts had higher phenol concentration than dichloromethane and hexane, and only ethyl acetate had highest flavonoid concentration, including gallic acid, luteolin, quercetin, catechin, and galangin [12]. Thus, ethyl acetate extract was further analyzed.…”

Section: Targeted Isolation Of Antioxidant Constituents From Plantagomentioning

confidence: 99%

“…Apigenin (P2) was isolated from ethyl acetate extract. gallic acid, luteolin, quercetin, catechin, and galangin [12]. Thus, ethyl acetate extract was further analyzed.…”

Section: Targeted Isolation Of Antioxidant Constituents From Plantagomentioning

confidence: 99%

Targeted Isolation of Antioxidant Constituents from Plantago asiatica L. and In Vitro Activity Assay

et al. 2020

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Plantago asiatica L. is widely distributed in Eastern Asia and a commonly used drug in China, Korea, and Japan for diuretic and antiphlogistic purposes. In this experiment, the present study was performed to isolate antioxidant molecules based on the DPPH scavenging activity assay and discover the bioactive compounds which contributed to performing the function of Plantago asiatica L. Each faction was chosen for further isolation guided by DPPH scavenging activity assay. Afterwards, two potential bioactive molecules, aesculetin and apigenin, were isolated for in vitro antioxidant activity in cells. Hydrogen-peroxide-induced oxidative stress led to decreased cell viability, impaired intercellular junction, and damage to the cell membrane and DNA. Furthermore, aesculetin ameliorated decreased cell viability induced by hydrogen peroxide via upregulation of antioxidant related genes, and apigenin also protected against H2O2 mainly by improving the glutathione (GSH) antioxidant system, such as increasing the activity of glutathione peroxidase (GPX), glutathione reductase (GR), and the ration of GSH/glutathione disulfide (GSSG). Above all, these findings suggest that aesculetin and apigenin may be bioactive compounds for antioxidant function in Plantago asiatica L.

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“…Ispaghula. Wound healing (Westerhof et al, 2001)Antiulcer (Singh et al, 2012) Bronchospasm (Hulbert et al, 1995) In vivo and in vitro In vitro Human Swellings (Avicenna, 1998;Khorasani, 2001) Lubricates the lung (Avicenna, 1998) Polydipsia (Avicenna, 1998;Khorasani, 2001) Fevers (Avicenna, 1998;Tonkaboni, 2007) (Mello et al, 2012) Wound healing (Thome et al, 2012;Zubair et al, 2012) Anti-inflammatory (Turel et al, 2009) Antiviral (Chiang et al, 2002) In vivo In vitro…”

Section: In Vivomentioning

confidence: 99%

Medicinal plants contain mucilage used in traditional Persian medicine (TPM)

Alipour

Heydarirad

Jafari

et al. 2014

Pharmaceutical Biology

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Hydroxyl scavenging activity accounts for differential antioxidant protection of Plantago major against oxidative toxicity in isolated rat liver mitochondria

Abstract: The potent antioxidant activity exhibited by P. major was able to prevent oxidative mitochondrial damage, contributing to the understanding of its hepatoprotective action against ROS-mediated toxicity.

Cited by 14 publications

References 41 publications

Protective Effect of Plantago major Extract against t-BOOH-Induced Mitochondrial Oxidative Damage and Cytotoxicity

Protective Effect of Plantago major Extract against t-BOOH-Induced Mitochondrial Oxidative Damage and Cytotoxicity

Targeted Isolation of Antioxidant Constituents from Plantago asiatica L. and In Vitro Activity Assay

Medicinal plants contain mucilage used in traditional Persian medicine (TPM)

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