Hydroxysafflor yellow A attenuates the expression of inflammatory cytokines in acute soft tissue injury

Dong, Fang; Xue, Changjiang; Wang, Yu; Peng, Yuanyuan; Zhang, Yadan; Jin, Ming; Zang, Baoxia

doi:10.1038/srep40584

Cited by 18 publications

(17 citation statements)

References 12 publications

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“…Sports-related muscle injuries in the lower extremities cause significant opportunity loss in training or competition, affecting exercise performance. The acute soft tissue injury is generally induced by external stress above a threshold, causing localized swelling, pain, bruising, blood capillary dilation, leukocyte infiltration, edema, and hemorrhage [2,3]. In this phase, neutrophils and macrophages rapidly invade injured muscle tissue and provide several important functions contributing to removal of damaged tissue, inflammation and healing [4].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Resveratrol Supplementation on Contusion Induced Muscle Injury

Hsu

Lee

et al. 2020

Int. J. Med. Sci.

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Muscle injuries frequently occur in contact sports events. The current treatment options for soft tissue injuries remain suboptimal and often result in delayed or incomplete recovery of damaged muscles. Resveratrol (RES) is a phenolic phytochemical, well-known for its antioxidant and anti-inflammatory properties. The purpose of this study is to evaluate the potential beneficial effects of RES supplementation on inflammation and regeneration in skeletal muscle after a contusion injury, in comparison to a conventional treatment of nonsteroidal anti-inflammatory drugs (NSAID). After one week of acclimation, forty eight-week-old male ICR mice were randomly divided into the five groups (n=8 per group): 1) normal control (NC), 2) mass-drop injury without any treatment (mass-drop injury, MDI), 3) post-injury NSAID treatment (MDI+ 10mg/kg NSAID), 4) post-injury RES supplementation (MDI+ 25mg/kg/day RES) and 5) post-injury treatment with RES and NSAID (MDI + resveratrol+ NSAID). After muscle contusion injury of the left gastrocnemius muscle, RES or NSAID were orally administered post-injury once a day for 7 days. Results showed that the MDI group had significantly higher serum uric acid (UA), CREA (creatinine), LDH (lactic dehydrogenase) and creatine kinase (CK) than the normal control group. Treatment with resveratrol reduced muscle damage as evidenced by the significantly decreased serum levels of UA, CREA, LDH and CK after contusion-induced muscle injuries in mice. In addition, RES and RES + NSAID groups promoted muscle satellite cell regeneration with increase in desmin protein after injury. Our results suggest that resveratrol combined with NSAID potentially improve muscle recovery and may be a potential candidate for further development as an effective clinical treatment for muscle repair.

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Section: Introductionmentioning

confidence: 99%

Protective Effects of Resveratrol Supplementation on Contusion Induced Muscle Injury

Hsu

Lee

et al. 2020

Int. J. Med. Sci.

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“…Hydroxysafflor yellow A (HSYA), the main water-soluble ingredient extracted from Carthami flos ( Carthamus tinctorius L.), is predominantly used in the treatment of cardiovascular diseases ( Li et al, 2019 ; Xu et al, 2019 ). Previous study reported that HSYA can resist inflammation, alleviate oxidation and apoptosis, improve neurological function, alleviate infarct volume, inhibit thrombosis formation and platelet aggregation, reduce brain edema, and protect blood vessels ( Han et al, 2016 ; Wang et al, 2016 ; Dong et al, 2017 ; Sun et al, 2018 ; Li et al, 2019 ). Recently, our laboratory found that HSYA has a protective effect on hypoxia/reoxygenation-induced cardiomyocyte damage in vitro , and the mechanism was related to NLRP3 inflammasome inhibition ( Ye et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Hydroxysafflor Yellow A Protects Against Myocardial Ischemia/Reperfusion Injury via Suppressing NLRP3 Inflammasome and Activating Autophagy

Ye¹,

Lu²,

Wang³

et al. 2020

Front. Pharmacol.

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Myocardial ischemia/reperfusion (MI/R) injury is a serious threat to human health. Hydroxysafflor yellow A (HSYA), the main water-soluble ingredient extracted from Carthami flos ( Carthamus tinctorius L.), has therapeutic potential for treating MI/R injury. However, the mechanisms of HSYA−mediated protection from MI/R injury are incompletely understood. In the present study, we investigated the effects and the underlying mechanisms of HSYA during MI/R. Adult Sprague-Dawley rats were subjected to left anterior descending artery ligation for 30 min followed by 24 h of reperfusion with or without HSYA treatment. The protective effect of HSYA was detected by 2,3,5-triphenyl tetrazolium chloride (TTC) staining, hematoxylin eosin (HE) staining, and myocardial enzymes detections. Serum levels of inflammatory factors such as TNF-α, interleukin (IL)-1β, and IL-18, were detected using ELISA kits. The expression of NLRP3 and other related proteins in the myocardium was detected by western blot and immunohistochemistry. The expression of autophagy-related proteins, including Atg5, BECN1, P62, and LC3B, was detected by western blot to evaluate the effect of HSYA on autophagy. Results showed that HSYA decreased the myocardial infarct size and attenuated the cardiac dysfunction in rats after I/R. In addition, HSYA inhibited myocardial apoptosis compared with the I/R group, decreased the levels of inflammatory cytokines in rat serum, reduced NLRP3 inflammasome expression, and induced autophagy. Mechanistically, our results demonstrated that HSYA can activate AMPK to improve autophagy and inhibit NLRP3 inflammasome by inhibiting the mTOR pathway. This work provides strong data supporting for the clinical applications of HSYA in MI/R injury.

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“…HSYA has been demonstrated to protect cardiovascular, cerebrovascular and nerve cells, and to prevent inflammation, oxidation and tumors (30)(31)(32)(33). As aforementioned, cisplatin is one of the most commonly used cancer chemotherapy drugs.…”

Section: Discussionmentioning

confidence: 99%

Hydroxyl safflower yellow A regulates the tumor immune microenvironment to produce an anticancer effect in a mouse model of hepatocellular carcinoma

Feng

Wang

et al. 2019

Oncol Lett

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Hepatocellular carcinoma (HCC) is a serious threat to human health. Chemotherapy drugs such as cisplatin are widely used in cancer treatment, but can cause severe side effects. Hydroxyl safflower yellow A (HSYA) is a water-soluble chalcone glycoside substance extracted from safflowers (Carthamus tinctorius L.) that has been reported to inhibit tumor growth with few side effects. The tumor immune microenvironment is crucial for the proliferation and invasiveness of tumor cells, and it is mediated by forkhead box P3-positive (FOXP3 +) regulatory T cells (Tregs) and retinoic acid receptor-related orphan receptor-γ (RORγ)-expressing Th17 cells. FOXP3 + Tregs inhibit immunoreaction and FOXP3 is a key indicator of Tregs. RORγ isoform 2, also known as RORγt, is an important transcription factor in Th17 cells that may promote cancer progression. In the present study, the antitumor effect of HSYA on HCC was investigated, as well as its impact on the tumor immune microenvironment. Following the establishment of a mouse model for HCC, hematoxylin and eosin staining were performed to observe histological changes in liver tumors, and the spleen and thymus were weighed to calculate the spleen and thymus indexes. The proportion of FOXP3 + Tregs in the spleen was determined by flow cytometry, and expression levels of Foxp3 and Rorγt were examined by reverse transcription-quantitative polymerase chain reaction and western blot analysis. The results of the present study showed that cisplatin inhibited tumor growth, caused weight loss and reduced the immunoreactivity of the mice. HSYA inhibited tumor growth without causing significant weight loss. The proportion of FOXP3-expressing Tregs in the spleen and the expression of Foxp3 and Rorγt mRNA decreased following treatment with certain doses of HSYA. In conclusion, HSYA inhibited tumor growth without detrimental effects on the weight of the mice, indicating that HSYA may be suitable as a novel therapy for HCC patients.

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Hydroxysafflor yellow A attenuates the expression of inflammatory cytokines in acute soft tissue injury

Cited by 18 publications

References 12 publications

Protective Effects of Resveratrol Supplementation on Contusion Induced Muscle Injury

Protective Effects of Resveratrol Supplementation on Contusion Induced Muscle Injury

Hydroxysafflor Yellow A Protects Against Myocardial Ischemia/Reperfusion Injury via Suppressing NLRP3 Inflammasome and Activating Autophagy

Hydroxyl safflower yellow A regulates the tumor immune microenvironment to produce an anticancer effect in a mouse model of hepatocellular carcinoma

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