Hyper-methylation of the upstream CpG island shore is a likely mechanism of GPER1 silencing in breast cancer cells

Manjegowda, Mohan C.; Gupta, Punit; Limaye, Anil Mukund

doi:10.1016/j.gene.2017.03.006

Cited by 26 publications

(38 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other studies investigating GPER mRNA expression in breast cancer have done so in comparison to normal mammary tissue to demonstrate lower staining in tumour tissue [ 25 ]. One of the largest studies to date reported GPER expression in 84 normal breast tissues and 781 primary breast tumours using TCGA RNAseq data accessed through the UCSC Cancer Genomics Browser; they demonstrated that GPER expression is lower in primary tumours than normal breast tissues and that higher GPER expression in breast cancer patients was associated with increased survival, which is in agreement with our findings [ 19 ].…”

Section: Discussionsupporting

confidence: 91%

“…High GPER protein expression is associated with increased distant disease free survival in ER-positive lymph node negative disease [ 15 ], presence of metastasis [ 16 ] and adverse relapse free survival of patients treated with tamoxifen [ 17 ]. GPER mRNA expression is significantly lower in tumour tissue in comparison to normal tissue, indicating that GPER acts as a tumour suppressor [ 18 , 19 ]. Recently, a large assessment of GPER mRNA expression in 781 primary breast tumours demonstrated that high GPER expression is associated with favourable overall survival and that GPER silencing may be due to hyper-methylation of the flanking regions of the upstream CpG island [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…GPER mRNA expression is significantly lower in tumour tissue in comparison to normal tissue, indicating that GPER acts as a tumour suppressor [ 18 , 19 ]. Recently, a large assessment of GPER mRNA expression in 781 primary breast tumours demonstrated that high GPER expression is associated with favourable overall survival and that GPER silencing may be due to hyper-methylation of the flanking regions of the upstream CpG island [ 19 ]. However a smaller study of 167 breast cancer patients showed no association between mRNA expression and patient survival [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Low expression of G protein-coupled oestrogen receptor 1 (GPER) is associated with adverse survival of breast cancer patients

et al. 2018

View full text Add to dashboard Cite

G protein-coupled oestrogen receptor 1 (GPER), also called G protein-coupled receptor 30 (GPR30), is attracting considerable attention for its potential role in breast cancer development and progression. Activation by oestrogen (17β-oestradiol; E2) initiates short term, non-genomic, signalling events both in vitro and in vivo. Published literature on the prognostic value of GPER protein expression in breast cancer indicates that further assessment is warranted. We show, using immunohistochemistry on a large cohort of primary invasive breast cancer patients (n=1245), that low protein expression of GPER is not only significantly associated with clinicopathological and molecular features of aggressive behaviour but also significantly associated with adverse survival of breast cancer patients. Furthermore, assessment of GPER mRNA levels in the METABRIC cohort (n=1980) demonstrates that low GPER mRNA expression is significantly associated with adverse survival of breast cancer patients. Using artificial neural networks, genes associated with GPER mRNA expression were identified; these included notch-4 and jagged-1. These results support the prognostic value for determination of GPER expression in breast cancer.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Low expression of G protein-coupled oestrogen receptor 1 (GPER) is associated with adverse survival of breast cancer patients

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Second: DNA methylation is recognized by specific Methyl-CpG binding proteins, such as MeCP2, that recruit co-repressor protein complexes and thereby mediate silencing (Nan et al, 1998). Aberrant methylation patterns at CpG islands and shores have been linked to human disease, including multiple cancers (Ohm et al, 2007; Irizarry et al, 2009; Berman et al, 2011; Manjegowda et al, 2017). …”

Section: The Role Of Dna Methylation In Disease and Agingmentioning

confidence: 99%

Implications of DNA Methylation in Parkinson’s Disease

Miranda-Morales

Meier

Sandoval-Carrillo

et al. 2017

Front. Mol. Neurosci.

View full text Add to dashboard Cite

It has been 200 years since Parkinson’s disease (PD) was first described, yet many aspects of its etiopathogenesis remain unclear. PD is a progressive and complex neurodegenerative disorder caused by genetic and environmental factors including aging, nutrition, pesticides and exposure to heavy metals. DNA methylation may be altered in response to some of these factors; therefore, it is proposed that epigenetic mechanisms, particularly DNA methylation, can have a fundamental role in gene–environment interactions that are related with PD. Epigenetic changes in PD-associated genes are now widely studied in different populations, to discover the mechanisms that contribute to disease development and identify novel biomarkers for early diagnosis and future pharmacological treatment. While initial studies sought to find associations between promoter DNA methylation and the regulation of associated genes in PD brain tissue, more recent studies have described concordant DNA methylation patterns between blood and brain tissue DNA. These data justify the use of peripheral blood samples instead of brain tissue for epigenetic studies. Here, we summarize the current data about DNA methylation changes in PD and discuss the potential of DNA methylation as a potential biomarker for PD. Additionally, we discuss environmental and nutritional factors that have been implicated in DNA methylation. Although the search for significant DNA methylation changes and gene expression analyses of PD-associated genes have yielded inconsistent and contradictory results, epigenetic modifications remain under investigation for their potential to reveal the link between environmental risk factors and the development of PD.

show abstract

“…In addition to the role of DNA methylation in long term silencing of genes and silencing of repetitive elements (e.g., transposons) [14], as well as X chromosome inactivation, it was also shown that DNA methylation plays an important role in the establishment and maintenance of imprinted genes, suppression of viral genes, and other deleterious elements that have been incorporated into the genome of the host over time as well as carcinogenesis [15,16].…”

mentioning

confidence: 99%

DNA Methylation as a Biomarker for Body Fluid Identification

Gomaa

Salehi

Behl

2017

AJFSFM

View full text Add to dashboard Cite

Sciences www.nauss.edu.sa http://ajfsfm.nauss.edu.sa Arab Journal of Forensic Sciences & Forensic Medicine to highlight the feasibility of using DNA methylation of certain genetic markers in body fluid identification and its implications in forensic investigations. The reviewed articles have employed molecular genetics techniques such as bisulfite sequencing PCR (BSP), methylation specific PCR (MSP), and Multiplex SNaPshot Microarray. Bioinformatics software such as Matrix Laboratory (MATLAB) and BiQ Analyzer has been used. Biological fluids have different methylation patterns which allows using DNA methylation to identify body fluids gives accurate results consuming minute amounts of precocious biological evidence material. Recent studies have incorporated next-generation sequencing (NGS) to discover more reliable markers that can differentiate between different body fluids. Nonetheless, new DNA methylation markers are yet to be discovered to accurately differentiate between saliva and vaginal secretions with high confidence. Epigenetic changes are dynamic, and it is important to find stable DNA sequences that can be used as reliable biomarkers for the identification of different types of body fluids encountered at a crime scene.

show abstract

Hyper-methylation of the upstream CpG island shore is a likely mechanism of GPER1 silencing in breast cancer cells

Cited by 26 publications

References 31 publications

Low expression of G protein-coupled oestrogen receptor 1 (GPER) is associated with adverse survival of breast cancer patients

Low expression of G protein-coupled oestrogen receptor 1 (GPER) is associated with adverse survival of breast cancer patients

Implications of DNA Methylation in Parkinson’s Disease

DNA Methylation as a Biomarker for Body Fluid Identification

Contact Info

Product

Resources

About