Hyperactive reactive oxygen species impair function of porcine Sertoli cells via suppression of surface protein ITGB1 and connexin-43

Zhang, Danchen; Chen, Rong; Cai, Yihui; Wang, Jingjing; Yin, Caixia; Zou, Kang

doi:10.24272/j.issn.2095-8137.2020.024

Cited by 12 publications

(3 citation statements)

References 25 publications

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“…In vitro evidence revealed overproduction of ROS suppress expression of Gja1 and Itgb1 in SCs can lead to germ cells losses (26). In addition, previous research demonstrated that heat stress changes the BTB dynamics.…”

Section: Real-time Pcr Analysismentioning

confidence: 97%

Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

Aghajanpour,

Soltani,

Afshar

et al. 2024

IJRM

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Background: An increase in the temperature of the testis is associated with damage to the epithelium of seminiferous tubules and disruption of sperm production. Objective: The current study aimed to investigate the effect of the Sertoli cell-conditioned medium (SCCM) on the blood-testis-barrier associated genes and spermatogenesis process following scrotal hyperthermia. Materials and Methods: In this experimental study, 40 adult NMRI mice (8 wk, 25–30 gr) were allocated into 4 groups: I) control, II) DMEM (10 μl Dulbecco’s Modified Eagle Medium), III) scrotal hyperthermia, and IV) scrotal hyperthermia+SCCM (10 μl SCCM). Hyperthermia was induced by placing the mice scrotum in water at 43°C for 20 min every other day for 10 days. Mice were treated every other day for 5 wk. Then the animals were euthanized, and the tails of epididymis were removed to analyze sperm parameters, testis were taken for stereological assessment, reactive oxygen spices and glutathione levels, and the expression of Ocln, Gja1, Cdh2, and Itgb1. Results: The results of sperm analysis indicated that SCCM-treated mice significantly increased sperm count and motility and reduced DNA fragmentation. In addition, histological and molecular findings showed that the volume of testicular tissue, the number of germ cells, the glutathione level, and the expression of Ocln, Gja1, Cdh2, and Itgb1 genes were significantly increased in the SCCM-treated mice. Conclusion: Findings suggest that growth factors of SCCM stimulate the proliferation and differentiation of germ cells through paracrine effects and upregulate the blood-testis-barrier-associated genes in mice subjected to scrotal hyperthermia. Key words: Testis, Sertoli cells, Culture media, Hyperthermia, Spermatogenesis.

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Section: Real-time Pcr Analysismentioning

confidence: 97%

Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

Aghajanpour,

Soltani,

Afshar

et al. 2024

IJRM

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“…After further enrichment of through differential attachment, ProSGs were purified and identified using IF staining against UCHL1(81.2±1.78%) and PLZF (73.09±1.56%) (Figure S1A-H). Meanwhile, Sertoli cells were purified using hypotonic treatment (Figure S1I-L) and inactivated using mitomycin C. Purified ProSGs were seed on mitosis-inactive Sertoli cells as an invitro co-culture system (Figure S1M-P) (Zhang et al, 2020). During in vitro culture, the morphology of the newly isolated cells was round and transparent.…”

Section: Isolation and Identification Of Porcine Prosgsmentioning

confidence: 99%

Cadherin-18 loss in prospermatogonia and spermatogonial stem cells enhances cell adhesion through a compensatory mechanism

Li,

Zhang,

Wang

et al. 2024

Zoological Research

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Extracellular membrane proteins play key roles in the interaction of testicular microenvironment germline stem cell (GSC) through mediating cell attachment, recognition, and signal transduction processes. Cadherin 18 (CDH18), a type II classical cadherin, is mainly expressed in the nervous and reproductive systems. Here we report the expression of CDH18 in the Prospermatogonia (ProSGs) of neonatal pigs and mouse spermatogonial stem cells (SSCs). Disruption of CDH18 expression showed no detrimental impact on cell morphology, proliferation, self-renewal or differentiation in cultured porcine ProSGs, but enhanced cell adhesion and prolonged maintenance. Transcriptomic analysis indicated that the downregulation of CDH18 in ProSGs significantly upregulated genes and signaling pathways associated with cell adhesion. To further reveal the function of CDH18 in germ cells, Cdh18 knockout mice were constructed, which exhibited normal testicular morphology, histology, and spermatogenesis. Moreover, transcriptomic data showed increased expression of genes associated with adhesion, consistent with those observed in porcine ProSGs. The interaction of CDH18 with β-catenin and JAK2 in both porcine ProSGs and murine SSCs indicated an inhibitory effect on the canonical Wnt and JAK-STAT signaling pathways during CDH18 deficiency. Collectively, our findings highlight the crucial role of CDH18 in regulating the cell adhesion in porcine ProSGs and mouse SSCs. These insights may have significant implications for understanding the regulatory mechanism of testicular niche.

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“…Moreover, our lab’s studies have revealed that TLRs’ expression influences the production of pro-inflammatory cytokines and the level of oxidative stress ( Deng et al, 2020 ; Wang et al, 2020 ). Alternatively, bacterial endotoxin lipopolysaccharide-induced ROS can inhibit Leydig cell steroidogenesis and cause Sertoli cell apoptosis ( Le Goffic et al, 2003 ; Zhang et al, 2020 ). Cumulative oxidative damage may inhibit steroid synthesis in Leydig cells, and abnormal mitochondria may also be associated with Leydig cell apoptosis, leading to decreased sex steroid hormones.…”

Section: Effects Of Oxidative Stress On Male Fertility During Bacterial Infectionmentioning

confidence: 99%

Bacterial Infections Affect Male Fertility: A Focus on the Oxidative Stress-Autophagy Axis

Wang

Zhang

Yao

et al. 2021

Front. Cell Dev. Biol.

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Numerous factors trigger male infertility, including lifestyle, the environment, health, medical resources and pathogenic microorganism infections. Bacterial infections of the male reproductive system can cause various reproductive diseases. Several male reproductive organs, such as the testicles, have unique immune functions that protect the germ cells from damage. In the reproductive system, immune cells can recognize the pathogen-associated molecular patterns carried by pathogenic microorganisms and activate the host’s innate immune response. Furthermore, bacterial infections can lead to oxidative stress through multiple signaling pathways. Many studies have revealed that oxidative stress serves dual functions: moderate oxidative stress can help clear the invaders and maintain sperm motility, but excessive oxidative stress will induce host damage. Additionally, oxidative stress is always accompanied by autophagy which can also help maintain host homeostasis. Male reproductive system homeostasis disequilibrium can cause inflammation of the genitourinary system, influence spermatogenesis, and even lead to infertility. Here, we focus on the effect of oxidative stress and autophagy on bacterial infection in the male reproductive system, and we also explore the crosslink between oxidative stress and autophagy during this process.

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Hyperactive reactive oxygen species impair function of porcine Sertoli cells via suppression of surface protein ITGB1 and connexin-43

Cited by 12 publications

References 25 publications

Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

Cadherin-18 loss in prospermatogonia and spermatogonial stem cells enhances cell adhesion through a compensatory mechanism

Bacterial Infections Affect Male Fertility: A Focus on the Oxidative Stress-Autophagy Axis

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