Hyperacute Serum has Markedly Better Regenerative Efficacy than Platelet-Rich Plasma in a Human Bone Oxygen–Glucose Deprivation Model

Abstract

A similar pro-proliferation effect was observed with recombinant growth factors, indicating that HAS may be an alternative agent for enhancing the regeneration of damaged bone cells.

The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method

“…The results were presented as relative values compared to the positive control for the secondary antibody. From the 138 cytokines and growth factors selected as known paracrine mediators of tissue regeneration, vascularization or inflammation, 82 proteins were neglected (AU < 2%) thus 56 proteins (AU > 2%) are presented in Figure 2A [35]. The whole data set containing the 138 cytokines and growth factors is shown in Table S1.…”

“…The presence of pro-inflammatory cytokines could further enhance inflammation in injured tissues. Further benefits of using HAS instead of EDTA PRP is the better cell proliferative effect on mesenchymal stem cells, osteoblasts, and osteoarthritic chondrocytes than EDTA PRP based on our previous results [32,34,35].…”

“…The level of angiogenic proteins [35] and cytokines was screened by semi-quantitative proteome profiler analysis. This method is not accurate enough for statistic comparison but was sufficient to determine that the overall concentration of proteins was the highest in EDTA PRP, followed by HAS, plasma, and the lowest was in serum.…”

“…Despite the positive results of HAS in cell proliferation and migration, the composition of HAS has not been reported so far. However, angiogenic proteins were measured earlier both in HAS and PRP by Proteome Profiler, showing that EDTA PRP has more angiopoietic components, whereas HAS has more anti-angiopoietic components [35]. In the present study we investigated and compared the composition of HAS, PRP, blood serum, and plasma to understand the differences in the cell proliferative and regenerative effect of HAS and PRP.…”

Redifferentiation of Articular Chondrocytes by Hyperacute Serum and Platelet Rich Plasma in Collagen Type I Hydrogels

“…Discrepancies among PRP preparations and the mode of activation remains a concern owing to the uncertainty of biological effects and clinical outcomes. A recent alternative derivative of platelet-rich fibrin (PRF) is hyperacute serum (HAS) and HAS which has been consistently reported for its regenerative capacity by increasing the cell proliferation capacity, as reported for chondrocytes [14], bone-marrow mesenchymal stem cells [15], and in an ex vivo model of bone ischemia recently [16]. HAS involves the activation of the natural coagulation cascade by a single-step centrifugation process, and its chemical composition comprises serum proteins, albumin, growth factor, and cytokines.…”