Hyperbaric Oxygen Therapy After Acute Thoracic Spinal Cord Injury

Falavigna, Asdrúbal; Figueiró, Manuela Peletti; Silva, Pedro Guarise da; Conzatti, Lucas Piccoli; Rizkalla, Elisa Braun; Santos, Scheila Cardoso dos; Quadros, Francine Würzius de; Radaelli, Lucas

doi:10.1097/brs.0000000000002387

Cited by 10 publications

(6 citation statements)

References 26 publications

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“…As the urethra of female rats is shorter compared with that of male rats, urination recovery and abdominal massage may be easier following SCI in female rats. Therefore, female rats were selected as an animal model of spinal cord injury to minimize the experimental error caused by urinary dysfunction that occurred when not using female rats alone, as observed in previous studies ( 47 , 64 , 65 ). In the early stage of spinal cord injury (1 week) in the present study, the rats received abdominal massages every 6 h to assist with emptying their bladders until independent urination was possible.…”

Section: Discussionmentioning

confidence: 99%

Hypoxic preconditioned bone mesenchymal stem cells ameliorate spinal cord injury in rats via improved survival and migration

et al. 2018

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The unique hypoxic inflammatory microenvironment observed in the spinal cord following spinal cord injury (SCI) limits the survival and efficacy of transplanted bone mesenchymal stem cells (BMSCs). The aim of the present study was to determine whether hypoxic preconditioning (HP) increased the therapeutic effects of BMSC on SCI. BMSCs were pretreated with cobalt chloride (CoCl2) in vitro, and the proliferative apoptotic and migratory abilities of these hypoxic BMSCs (H-BMSCs) were assessed. BMSCs and H-BMSCs derived from green fluorescent protein (GFP) rats were transplanted into SCI rats in vivo. The neurological function, histopathology, inflammation, and number and migration of transplanted cells were examined. HP significantly enhanced BMSC migration (increased hypoxia inducible factor 1α and C-X-C motif chemokine receptor 4 expression) and tolerance to apoptotic conditions (decreased caspase-3 and increased B-cell lymphoma 2 expression) in vitro. In vivo, H-BMSC transplantation significantly improved neurological function, decreased spinal cord damage and suppressed the inflammatory response associated with microglial activation. The number of GFP-positive cells in the SCI core and peripheral region of H-BMSC animals was increased compared with that in those of BMSC animals, suggesting that HP may increase the survival and migratory abilities of BMSCs and highlights their therapeutic potential for SCI.

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Section: Discussionmentioning

confidence: 99%

Hypoxic preconditioned bone mesenchymal stem cells ameliorate spinal cord injury in rats via improved survival and migration

et al. 2018

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“…Studied indicated that HBOP has positive effects on traumatic brain injury, ischemic stroke, and spinal cord injury . Recently, there is also discovered that HBOP provides neuroprotective effect on ICH .…”

Section: Discussionmentioning

confidence: 99%

“…Studied indicated that HBOP has positive effects on traumatic brain injury, 1,20 ischemic stroke, 21,22 and spinal cord injury. 2,3 Recently, there is also discovered that HBOP provides neuroprotective effect on ICH. 6,7 HBOP-induced activation of ribosomal protein S6 kinases (p70 S6 K), an important enzyme in protein synthesis, causes HO-1 upregulation and reduces brain edema.…”

Section: Discussionmentioning

confidence: 99%

“…Intracerebral hemorrhage (ICH), a devastating stroke subtype, is characterized by high mortality and disability without an effective treatment. Hyperbaric oxygen preconditioning (HBOP) provides neuroprotective effects in ischemia/hypoxia, trauma, and spinal cord injury . Increasing evidences suggest that HBOP also plays beneficial role in ICH …”

Section: Introductionmentioning

confidence: 99%

“…Hyperbaric oxygen preconditioning (HBOP) provides neuroprotective effects in ischemia/hypoxia, trauma, and spinal cord injury. [1][2][3][4] Increasing evidences suggest that HBOP also plays beneficial role in ICH. 5,6 Studies suggest that inflammatory reaction is an important mechanism of secondary injury after ICH, which is the critical factor of high mortality and disability after ICH.…”

Section: Introductionmentioning

confidence: 99%

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Hyperbaric oxygen preconditioning attenuates brain injury after intracerebral hemorrhage by regulating microglia polarization in rats

et al. 2019

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Aims Hyperbaric oxygen preconditioning (HBOP) attenuates brain edema, microglia activation, and inflammation after intracerebral hemorrhage (ICH). In this present study, we investigated the role of HBOP in ICH‐induced microglia polarization and the potential involved signal pathway. Methods Male Sprague‐Dawley rats were divided into three groups: SHAM, ICH, and ICH + HBOP group. Before surgery, rats in SHAM and HBOP groups received HBO for 5 days. Rats in SHAM group received needle injection, while rats in ICH and ICH + HBOP groups received 100 μL autologous blood injection into the right basal ganglia. Rats were euthanized at 24 hours after ICH, and the brains were removed for immunohistochemistry and Western blotting. Neurological deficits and brain water content were determined. Results Intracerebral hemorrhage induced brain edema, which was significantly lower in the HBOP group. The levels of MMP9 were also less in the HBOP group. HBO pretreatment resulted in less neuronal death and neurological deficits after ICH. Their immunoactivity and protein levels of M1 markers were downregulated, but the M2 markers were unchanged by HBOP. In addition, ICH‐induced pro‐inflammatory cytokine (TNF‐α and IL‐1β) levels and the phosphorylation of JNK and STAT1 were also lower in the HBOP rats. Conclusions HBO pretreatment attenuated ICH‐induced brain injuries and MMP9 upregulation, which may through the inhibiting of M1 polarization of microglia and inflammatory signal pathways after ICH.

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