Hyperbaric pressure of 51 atmospheres is without effect on the depression of oxygen uptake in kidney tissue culture produced by halothane

Cohen, Peter J.; Bedows, Elliott; Brabec, Michael J.; Knight, Paul R.

doi:10.1016/0024-3205(85)90133-x

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…Finally, to better understand the cellular mechanisms of volatile anesthetic action, hyperbaric pressure experiments may be promising as high atmospheric pressures are shown to reverse many volatile anesthetics actions. 40 …”

Section: Discussionmentioning

confidence: 99%

Sevoflurane-mediated modulation of oxidative myocardial injury

Sedghi,

Khadra,

Pourafkari

et al. 2023

J Cardiovasc Thorac Res

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Introduction: Volatile anesthetics offer protection when administered throughout an ischemic injury. We examined how volatile anesthetics modulate the cardiac myocytic injury associated with hydrogen peroxide. Methods: Forty-eight Long-Evans rats were divided into four groups depending on the treatment: none (CONT), Glibenclamide (GLB); Sevoflurane (SEV); or GLB+SEV. Each group was further divided into two, one of which was exposed to hydrogen peroxide (H2 O2 ). Oral GLB was administered 48 hours before myocardial isolation. All rats were anesthetized by intraperitoneal injection of Ketamine, and the hearts were harvested after heparinization. Cardiomyocytes were isolated using a combination of mechanical mincing and enzymatic digestion. After isolation, the aliquots of cells were exposed to H2 O2 and FeSO4 for 30 minutes. The cell suspensions were then bubbled for 10 minutes with 100% oxygen and 1.5% SEV if appropriate. Apoptosis was detected by fluorescein-bound annexin-V (ANX-V), necrosis by propidium iodide, and ELISA assessed caspase-3 activity in all groups. Results: There was an increase in apoptosis, necrosis, and caspase-3 activity in the cells following exposure to hydrogen peroxide. SEV reduced the rate of cell necrosis and apoptosis. Pretreatment with GLB did not alter the effects of SEV. Similarly, caspase-3 activity did not change with GLB, although SEV administration reduced this enzymatic activity in response to hydrogen peroxide. Conclusion: In this oxidant injury model, we demonstrated that incubating isolated cardiomyocytes with SEV profoundly diminished H2 O2 -induced apoptotic and necrotic cells compared to their CONTs. These results support the hypothesis that KATP channels are not the sole mediators associated with anesthetic preconditioning.

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Section: Discussionmentioning

confidence: 99%

Sevoflurane-mediated modulation of oxidative myocardial injury

Sedghi,

Khadra,

Pourafkari

et al. 2023

J Cardiovasc Thorac Res

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Hyperbaric pressure does not affect the analgesia produced by nitrous oxide in the mouse

Cohen

1989

Can J Anaesth

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14: 48-9, 1987. Supported in part by NIH Grant GM 29628.The ability of increased pressure to reverse unconsciousness produced by administration of general anaesthetics (pressure reversal) has been well documented since its first description over 40 years ago) Anaesthetic actions amenable to pressure reversal include loss of the righting reflex in the mouse produced by both nitrous oxide 2 (25 ata of helium increased EDso by 15 per cent) and isoflurane 3 (12.5 ata was associated with a 27 per cent increase in EDso) recovery of swimming in tadpoles exposed to 0.25 per cent chloroform 4 (complete narcotic reversal was observed at a hydrostatic pressure of 34 ata), and inhibition of axonal transmission in the rat superior cervical ganglion s (35 ata reduced the inhibition produced by 2,4 per cent halothane by 30 per cen0. However, pressure does not antagonize all phenomena associated with general anaesthesia. For example, halothane's inhibition of synaptic transmission in the rat superior cervical ganglion is potentiated, rather than antagonized, by pressure; a decrease of five to ten per cent in post-ganglionic action potential is produced by 35 ata of helium, both in the presence and absence of halothane.5 Similarly, halothane's depression of the ciliary beat of Tetrahymena pyriformis is enhanced by 137 ata. 6 Recently, it has been reported that halothane's inhibition of the oxygen uptake of rat liver mitochondria 7 and intact green monkey kidney cells 8 is not reversed by 50 ata of hydraulic pressure.While there have been numerous studies of the action of pressure on anaesthetic-induced unconsciousness or loss of the righting refex, its effect on other components of general anaesthesia (such as analgesia or suppression of reflex activity) has not been examined. Accordingly, the following study was undertaken.Abbreviation: ata = atmospheres absolute. Ambient pressure is 1 ata = 101.3 kPa (760 mmHg; 14.7 lb. in -2) CAN J ANAESTH 1989 / 36:1 / pp40-3

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Hyperbaric pressure of 51 atmospheres is without effect on the depression of oxygen uptake in kidney tissue culture produced by halothane

Cited by 2 publications

References 14 publications

Sevoflurane-mediated modulation of oxidative myocardial injury

Sevoflurane-mediated modulation of oxidative myocardial injury

Hyperbaric pressure does not affect the analgesia produced by nitrous oxide in the mouse

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