Hyperbranched Poly‐L‐Lysine‐Based Water‐Insoluble Complexes as Antibacterial Agents with Efficient Antibacterial Activity And Cytocompatibility

Abstract

Despite the advances in technology, bacterial infection associated with biomedical devices is still one of the most challenging issues in clinical practice. Incorporation of antimicrobial agents is regarded as an efficient way to combat medical device associated infectious. However, most of antimicrobial agents have high toxicity to host cells. Thus, fabrication of novel antimicrobial agents that simultaneously fulfil the requirements of antibacterial activity as well as biocompatibility is urgently needed. Herein, a series of water‐insoluble antibacterial complexes based on hyperbranched poly‐L‐lysine (HBPL) and four different surfactants through non‐covalent interactions were developed. Such kinds of surfactants had great effects on the antibacterial property of poly(ɛ‐caprolactone) (PCL) films that incorporated with the HBPL‐based complexes. The results revealed that the PCL films that doped with HBPL/phosphate ester surfactant complexes showed the highest bacterial killing efficiency. Moreover, the cytocompatibility of the composite films was also investigated. Hemolysis experiments indicated that all the films had low hemolytic activities. Considering the excellent antimicrobial and cytocompatibility properties, we believe that the optimized complexes have great potential to be used as antimicrobial agents in biomedical field.This article is protected by copyright. All rights reserved

Fabrication of Oleophilic Polypeptide Nanoparticle from Complexing of Cross-Linked Epsilon-poly-l-lysine with Docusate Sodium for Preparation of Bactericidal Thermoplastic Polyurethanes

“…The CPL/DS nanoparticles were prepared through a modified method according to our previous report . Briefly, 0.1 g of CP was added to 10 mL of hydrochloride solution (0.2 M HCl) and then stirred at room temperature until realizing the homogeneous dispersion.…”

“…The CPL/DS nanoparticles were prepared through a modified method according to our previous report. 31 Briefly, 0.1 g of CP was added to 10 mL of hydrochloride solution (0.2 M HCl) and then stirred at room temperature until realizing the homogeneous dispersion. The anionic surfactant (DS) was dissolved in 60% (v/v) ethanol and then added dropwise to the above CPL solution at room temperature.…”

“…We first synthesized cross-linked epsilon-poly- l -lysine by using hexamethylene diisocyanate (HDI) as a cross-linking agent to form a urea linkage. Inspired by the idea of polyelectrolyte–surfactant complexes, − we then used docusate sodium, an ionic compound, to change the surface characteristics of the CPL nanoparticle, thus favoring its dispersion in an organic solvent. As expected, the CPL/DS nanoparticle that can be dispersed in organic solvent was obtained and characterized.…”