Hypericum roeperianum bark extract suppresses breast cancer proliferation via induction of apoptosis, downregulation of PI3K/Akt/mTOR signaling cascade and reversal of EMT

Guefack, Michel-Gael F.; Talukdar, Debojit; Mukherjee, Rimi; Guha, Subhabrata; Mitra, Debarpan; Saha, Depanwita; Das, Gaurav; Damen, François; Kuete, Victor; Murmu, Nabendu

doi:10.1016/j.jep.2023.117093

Cited by 6 publications

(2 citation statements)

References 54 publications

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“…To assess the change in mitochondrial membrane potential (MMP) induced by RTME treatment, flow cytometry analysis was performed using JC-1 stain, following a previously established protocol (Guefack et al, 2024) with few modifications. In brief, 2 × 10 8 cells/well were cultured overnight and treated with 11.9 µg/mL and 23.8 µg/mL concentrations of RTME.…”

Section: Mitochondrial Membrane Potential (δψM) Estimation By Jc-1 St...mentioning

confidence: 99%

“…For quantification of gene expression levels related to apoptosis, qRT-PCR was performed following a previously established protocol (Guefack et al, 2024) with few modifications. In brief, 1 × 10 6 cells/well were cultured overnight and treated with 11.9 µg/mL and 23.8 µg/mL concentrations of RTME.…”

Section: Gene Expression Analysis By Quantitative Real-time Pcr (Qrt-...mentioning

confidence: 99%

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Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Guha,

Talukdar,

Mandal

et al. 2024

Preprint

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Ethnophamacological relevance: In the traditional folklore medicine system, the primary uses of Ruellia tuberosa L. include as a diuretic, anti-hypertensive, antipyretic, anti-diabetic, analgesic, and gastroprotective agent. Some reports also demonstrated that it has been used to treat gonorrhea-like diseases. Purpose: Exploring the anti-cancer potential of the methanolic extract of Ruellia tuberosa L. flower (RTME) with special emphasis on human triple-negative breast cancer (TNBC) and investigating the possible signaling networks and regulatory pathways underlying it. Methods: Preparation of RTME and identifying the possible phytochemicals through GC-MS analysis. The anti-cancer potential of RTME was executed through in-vitro cytotoxicity assay, clonogenic assay, wound healing assay, ROS generation assay, cell cycle arrest, apoptotic nuclear morphology study, cellular apoptosis study, mitochondrial membrane potential (MMP) alteration study, protein and gene expressions alteration study. Apart from this, toxicological status and in-silico molecular docking studies were also conducted. Results: From this study, it was obvious that several phytochemicals within RTME have the potential to act as anti-cancerous agents. RTME was found to exhibit significant in-vitro cytotoxicity along with a reduction in colony formation and inhibition of cell migratory potential in MDA-MB-231 cells. RTME also induced intracellular ROS, promoted G0/G1 cell cycle arrest, caused mitochondrial membrane potential (MMP) alteration, and promoted cell death. From the pro- and anti-apoptotic marker study through the western blot and the qRT-PCR analysis, it was revealed that RTME promoted the intrinsic pathway of apoptosis. Furthermore, blood parameters and histological analysis revealed that RTME does not exhibit any toxic effect on female Balb/C mice. Finally, an in-silico molecular docking study revealed that the three identified lead phytochemicals in RTME show strong receptor-ligand interactions with the anti-apoptotic Bcl-2 and give a clue to the possible molecular mechanism of the RTME extract. Conclusion: From the findings, it was concluded that RTME has a significant therapeutic potential against TNBC which could be an alternative option for anti-cancer drug development.

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Section: Mitochondrial Membrane Potential (δψM) Estimation By Jc-1 St...mentioning

confidence: 99%

Section: Gene Expression Analysis By Quantitative Real-time Pcr (Qrt-...mentioning

confidence: 99%

Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Guha,

Talukdar,

Mandal

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

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Cancer Stem Cells: Robust Features and Therapeutic Targets

Abu-Serie,

Sarhan

2024

Interdisciplinary Cancer Research

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A novel normonoterpene glycoside and a new benzophenone derivative from Hypericum cerastioides and their in vitro cytotoxic activities

Konya Konuk,

Aru,

Öztürk

et al. 2024

Fitoterapia

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Hypericum roeperianum bark extract suppresses breast cancer proliferation via induction of apoptosis, downregulation of PI3K/Akt/mTOR signaling cascade and reversal of EMT

Cited by 6 publications

References 54 publications

Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Cancer Stem Cells: Robust Features and Therapeutic Targets

A novel normonoterpene glycoside and a new benzophenone derivative from Hypericum cerastioides and their in vitro cytotoxic activities

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