Hyperoxia impairs pro-angiogenic RNA production in preterm endothelial colony-forming cells

Ahern, Megan; Black, Claudine P.; Seedorf, Gregory J.; Baker, Christopher D.; Shepherd, Douglas P.

doi:10.3934/biophy.2017.2.284

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…We realized three-wavelength SIM using the 473 nm, 532 nm, and 635 nm channels to image β -actin, HIF, and FGF18 labelled with Alexa Fluor 488, Alexa Fluor 555, and Alexa Fluor 647 in fixed primary rat endothelial cells. We have previously studied angiogenic signaling in primary rat, sheep, and human endothelial cells using high-throughput multi-wavelength widefield microscopy (38–40).…”

Section: Resultsmentioning

confidence: 99%

Multicolor structured illumination microscopy and quantitative control of polychromatic coherent light with a digital micromirror device

Brown

Kruithoff

Seedorf

et al. 2020

Preprint

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We demonstrate structured illumination microscopy (SIM) at three wavelengths using a digital mirror device (DMD), an approach which has not previously been realized due to the DMD’s blazed grating effect. Previous approaches to SIM using a DMD have used one color of coherent light or multiple colors via incoherent projection. To address this challenge, we develop a forward model of DMD diffraction, a forward model of coherent pattern projection, and identify an experimentally feasible configuration for three common fluorophore wavelengths. Based on these results, we constructed a custom DMD SIM and created an open-source software suite to simulate, calibrate, execute, and reconstruct 2D linear SIM data. We validate this approach by demonstrating resolution enhancement for known calibration samples and fixed cells.

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Section: Resultsmentioning

confidence: 99%

Multicolor structured illumination microscopy and quantitative control of polychromatic coherent light with a digital micromirror device

Brown

Kruithoff

Seedorf

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…We realized three-wavelength SIM using the 473 nm, 532 nm, and 635 nm channels to image β-actin, HIF, and FGF18 labeled with Alexa Fluor 488, Alexa Fluor 555, and Alexa Fluor 647 in fixed primary rat endothelial cells. We have previously studied angiogenic signaling in primary rat, sheep, and human endothelial cells using high-throughput multi-wavelength widefield microscopy [20][21][22].…”

Section: Otf Measurement Techniquementioning

confidence: 99%

Supplementary document for Multicolor structured illumination microscopy and quantitative control of polychromatic light with a digital micromirror device - 5251324.pdf

Brown,

Kruithoff,

Seedorf

et al. 2021

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Hyperoxia impairs pro-angiogenic RNA production in preterm endothelial colony-forming cells

Cited by 2 publications

References 29 publications

Multicolor structured illumination microscopy and quantitative control of polychromatic coherent light with a digital micromirror device

Multicolor structured illumination microscopy and quantitative control of polychromatic coherent light with a digital micromirror device

Supplementary document for Multicolor structured illumination microscopy and quantitative control of polychromatic light with a digital micromirror device - 5251324.pdf

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