Hyperprolactinemia modifies extracellular matrix components associated with collagen fibrillogenesis in harderian glands of non- and pregnant female mice

Araujo, Ariadne S.L.; Simões, Manuel de Jesus; Araujo-Jr, Osvaldo P.; Simões, Ricardo Santos; Baracat, Edmund Chada; Nader, Helena B.; Soares-Jr, José Maria; Gomes, Regina C.T.

doi:10.1016/j.exer.2023.109612

Cited by 2 publications

(1 citation statement)

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“…Vaginal smears were performed daily, and the presence of sperm and vaginal epithelial cells in the smear was considered a successful mating and was recorded as day 1 of pregnancy. Five groups of pregnant rats were randomly assigned ( n = 6) 14 : control group, model group (PE), low-dose EG group (0.535 g/kg), medium-dose EG group (1.07 g/kg), and high-dose EG group (2.14 g/kg).…”

Section: Methodsmentioning

confidence: 99%

Eucommia granules activate Wnt/β-catenin pathway, and improve oxidative stress, inflammation, and endothelial injury in preeclampsia rats

Huang,

Xing,

Zhang

et al. 2024

Acta Cir. Bras.

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Purpose: Pre-eclampsia (PE) is a pregnancy-related complication. Eucommia is effective in the treatment of hypertensive disorders in pregnancy, but the specific effects and possible mechanisms of Eucommia granules (EG) in PE remain unknown. The aim of this study was to investigate the effects and possible mechanisms of EG in PE rats. Methods: Pregnant Sprague Dawley rats were divided into five groups (n = 6): the control group, the model group, the low-dose group, the medium-dose group, and the high-dose group of EG. The PE model was established by subcutaneous injection of levonitroarginine methyl ester. Saline was given to the blank and model groups, and the Eucommia granules were given by gavage to the remaining groups. Blood pressure and urinary protein were detected. The body length and weight of the pups and the weight of the placenta were recorded. Superoxide dismutase (SOD) activity and levels of malondialdehyde (MDA), placental growth factor (PIGF), and soluble vascular endothelial growth factor receptor-1 (sFIt-1) were measured in the placenta. Pathological changes were observed by hematoxylin-eosin staining. Wnt/β-catenin pathway-related protein expression was detected using Western blot. Results: Compared with the model group, the PE rats treated with EG had lower blood pressure and urinary protein. The length and weight of the pups and placental weight were increased. Inflammation and necrosis in the placental tissue was improved. SOD level increased, MDA content and sFIt-1/PIGF ratio decreased, and Wnt/β-catenin pathway-related protein expression level increased. Moreover, the results of EG on PE rats increased with higher doses of EG. Conclusions: EG may activate the Wnt/β-catenin pathway and inhibit oxidative stress, inflammation, and vascular endothelial injury in PE rats, thereby improving the perinatal prognosis of preeclamptic rats. EG may inhibit oxidative stress, inflammation, and vascular endothelial injury through activation of the Wnt/β-catenin pathway in preeclampsia rats, thereby improving perinatal outcomes in PE rats.

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Section: Methodsmentioning

confidence: 99%