Hyperspectral digital holography of microobjects

Abstract

Novel method is suggested for a hyperspectral wave field holographic recording, based on asymmetrical Fourier spectrometer with a flat microobject placed in one of its arms. The output signal, which is the interference of the reference field with the field diffracted by the object, is registered by CCD. The process of recording is reduced to consecutive registration of two-dimensional interferograms by changing the optical length of the reference arm of the interferometer. One-dimensional Fourier transform of the interferogram in each pixel gives a spatial distribution of the complex amplitude for all spectral components of a hyperspectral object field. Inverse Fresnel transform of this field gives a hyperspectral object field in the object plane. Hyperspectral amplitude and average-phase profile images of standard microscope samples obtained experimentally are presented. Coloring, Fellgett's advantage and speckle noise reduction are discussed.

Deep learning‐based color holographic microscopy

“…To achieve increased color accuracy using coherent imaging systems, a computational hyperspectral imaging approach can be used [8]. However, such systems typically require engineered illumination, such as a tunable laser to efficiently sample the visible band.…”