Hyperspectral imaging and multivariate analysis in the dried blood spots investigations

Abstract

The aim of this study was to apply a new methodology using the combination of the hyperspectral imaging and the dry blood spot (DBS) collecting. Application of the hyperspectral imaging is fast and non-destructive. DBS method offers the advantage also on the micro-invasive blood collecting and low volume of required sample. During experimental step, the reflected light was recorded by two hyperspectral systems. The collection of 776 spectral bands in the VIS-NIR range (400-1000 nm) and 256 spectral bands in the SWIR range (970-2500 nm) was applied. Pixel has the size of 8 × 8 and 30 × 30 µm for VIS-NIR and SWIR camera, respectively. The obtained data in the form of hyperspectral cubes were treated with chemometric methods, i.e., minimum noise fraction and principal component analysis. It has been shown that the application of these methods on this type of data, by analyzing the scatter plots, allows a rapid analysis of the homogeneity of DBS, and the selection of representative areas for further analysis. It also gives the possibility of tracking the dynamics of changes occurring in biological traces applied on the surface. For the analyzed 28 blood samples, described method allowed to distinguish those blood stains because of time of apply.

HyTexiLa: High Resolution Visible and Near Infrared Hyperspectral Texture Images

“…Hyperspectral imaging is widely used in a number of applications, e.g., remote sensing [ 14 ], medical diagnosis [ 15 ], cultural heritage [ 16 , 17 ], face recognition [ 18 ], food quality control [ 19 ], color management and printing [ 20 , 21 ]. A number of applications have been developed through the multivariate analysis of hyperspectral images, including the investigation of dried blood spots [ 22 ], the measurement of oil and protein contents in peanut varieties [ 23 ], the classification of muscle foods [ 24 ], the detection of defects on peaches [ 25 ] and numerous other applications.…”

Direct analysis from dried blood spot card surfaces with direct probe mass spectrometry – Evaluation study

“…Moreover, the scale placed in Figure B enables the size of the spaces between cellulose fibers to be estimated. The pores reach a few tens of micrometers in width (30 μm) which is more than the size of blood elements such as erythrocytes (ca 7 μm), so they may easily penetrate the interior of the DBS card.…”

Prediction of Microbial Spoilage and Shelf-Life of Bakery Products Through Hyperspectral Imaging

“…Each pixel of a hypercube shows a complete spectrum and the reaction of each wavelength band corresponding to that pixel. HSI has been used for decades in remote sensing [23], [24] while later it became an eminent technology in many other fields such as; food quality and assessment [13], [25], [26], forensic [27], medical [28], defence and home security [29], etc.…”