Hyphenated MS-based targeted approaches in metabolomics

Begou, Olga; Gika, Helen; Wilson, Ian D.; Theodoridis, Georgios

doi:10.1039/c7an00812k

Cited by 85 publications

(38 citation statements)

References 128 publications

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“…9 Moreover, LC-MS targeted analysis (i.e., analysis of specific metabolites) may be a powerful tool for plant metabolomics studies. 10 This work focuses on targeted analysis leading to the identification of further β-carboline alkaloids from sweet (P. alata) and sour (P. edulis) pulp and seeds by UHPLC-MS techniques and the quantification of harmane and harmine in sweet fruits by SBSE(PDMS)-LC/Flu.…”

Section: Introductionmentioning

confidence: 99%

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

Freire

Silva

Yariwake

2017

J. Braz. Chem. Soc.

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Samples of pulp and seeds of sour (P. edulis) and sweet (P. alata) passionfruit were subjected to targeted analysis to identify β-carboline alkaloids, using combined UHPLC-MS (ultrahigh performance liquid chromatography-mass spectrometry) and SBSE(PDMS) (stir bar sorption extraction using polydimethylsiloxane as stationary phase) for sample preconcentration. All the samples contained harmane and harmine; harmaline was found only in P. alata seeds and in P. edulis pulp, while harmalol was found only in P. alata seeds. An analysis of sweet passionfruit by SBSE(PDMS) combined with LC/Flu (liquid chromatography/fluorescence detection) indicated 1.03 × 10 -1 ± 3.05 × 10 -3 µg harmane L -1 pulp and 7.44 × 10 -5 ± 2.55 × 10 -6 µg harmane g -1 seeds, but harmine was not detectable by the quantitative method. Our findings underscore the need for further studies about passionfruit food safety, in view of the potential toxicity of β-carboline alkaloids.

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Section: Introductionmentioning

confidence: 99%

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

Freire

Silva

Yariwake

2017

J. Braz. Chem. Soc.

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“…Currently, the development of interfaces as the sheathless interface improve the sensitivity in the metabolomic analysis [9]. Nonetheless, CE-MS is a good alternative and has proved to be useful in the analysis of polar and charged metabolites in biological samples (urine, plasma, serum, or tissues) and endogenous compounds, such as nucleotides and amino acids [11]. For this reason, CE-MS is considered an orthogonal technique to LC-MS, which requires minute amounts of the sample [11].…”

Section: Introductionmentioning

confidence: 99%

A Non-Targeted Capillary Electrophoresis-Mass Spectrometry Strategy to Study Metabolic Differences in an In Vitro Model of High-Glucose Induced Changes in Human Proximal Tubular HK-2 Cells

et al. 2020

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Diabetic nephropathy is characterized by the chronic loss of kidney function due to high glucose renal levels. HK-2 proximal tubular cells are good candidates to study this disease. The aim of this work was to study an in vitro model of high glucose-induced metabolic alterations in HK-2 cells to contribute to the pathogenesis of this diabetic complication. An untargeted metabolomics strategy based on CE-MS was developed to find metabolites affected under high glucose conditions. Intracellular and extracellular fluids from HK-2 cells treated with 25 mM glucose (high glucose group), with 5.5 mM glucose (normal glucose group), and with 5.5 mM glucose and 19.5 mM mannitol (osmotic control group) were analyzed. The main changes induced by high glucose were found in the extracellular medium where increased levels of four amino acids were detected. Three of them (alanine, proline, and glutamic acid) were exported from HK-2 cells to the extracellular medium. Other affected metabolites include Amadori products and cysteine, which are more likely cause and consequence, respectively, of the oxidative stress induced by high glucose in HK-2 cells. The developed CE-MS platform provides valuable insight into high glucose-induced metabolic alterations in proximal tubular cells and allows identifying discriminative molecules of diabetic nephropathy.

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“…Currently, the two main techniques employed for (global) metabolic profiling studies are MS hyphenated to LC and NMR spectroscopy. Within the metabolomics field, CE‐MS especially emerged as a useful analytical technique for the profiling of (highly) polar and charged metabolites .…”

Section: Introductionmentioning

confidence: 99%

CE‐MS for metabolomics: Developments and applications in the period 2016–2018

2018

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In the field of metabolomics, CE-MS is now recognized as a strong analytical technique for the analysis of (highly) polar and charged metabolites in a wide range of biological samples. Over the past few years, significant attention has been paid to the design and improvement of CE-MS approaches for (large-scale) metabolic profiling studies and for establishing protocols in order to further expand the role of CE-MS in metabolomics. In this paper, which is a follow-up of a previous review paper covering the years 2014-2016 (Electrophoresis 2017, 38, 190-202), main advances in CE-MS approaches for metabolomics studies are outlined covering the literature from July 2016 to June 2018. Aspects like developments in interfacing designs and data analysis tools for improving the performance of CE-MS for metabolomics are discussed. Representative examples highlight the utility of CE-MS in the fields of biomedical, clinical, microbial, and plant metabolomics. A complete overview of recent CE-MS-based metabolomics studies is given in a table, which provides information on sample type and pretreatment, capillary coatings and MS detection mode. Finally, some general conclusions and perspectives are given.

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Hyphenated MS-based targeted approaches in metabolomics

Cited by 85 publications

References 128 publications

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

A Non-Targeted Capillary Electrophoresis-Mass Spectrometry Strategy to Study Metabolic Differences in an In Vitro Model of High-Glucose Induced Changes in Human Proximal Tubular HK-2 Cells

CE‐MS for metabolomics: Developments and applications in the period 2016–2018

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