Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology

Prochowska, Sylwia; Niżański, Wojciech; Fontbonne, Alain

doi:10.3390/ani12070903

Cited by 12 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The toxic effects of ROS were lipid peroxidation, protein ionization and inactivation, and DNA damage (Juan et al, 2021). After the addition of the hypo-osmotic solution, the damaged spermatozoa plasma membrane is seen straight tail shape (Prochowska et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke

Silviani¹,

Sukmanadi²,

Kurnijasanti³

et al. 2022

Ovz

View full text Add to dashboard Cite

Cigarette smoke chemicals caused oxidative stress by increasing Reactive Oxygen Species (ROS). ROS exposure could be neutralized by antioxidants, such as green tea (Camellia sinensis) leaf extract. This study aimed to determine the effect of green tea extract (GTE) on spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice (Mus musculus) exposed to cigarette smoke. Twenty-five mice were randomly divided into five groups. Group C- mice were given a placebo (1% Sodium carboxymethyl cellulose, Na-CMC). Group C+, T1, T2, and T3 mice were exposed to cigarette smoke and given 0, 20, 40, and 60 mg/kg BW GTE respectively. Cigarette smoke exposure used a clove cigarette per day. GTE in 1% Na-CMC solution was administered at 0.5 mL orally using a gastric probe. The treatment was conducted daily for 36 days, and on day 37, all mice were euthanized for spermatozoa evaluation. The results showed that all parameters evaluated in the C+ group were lower (p <0.05) than in C- group. Administration of GTE in the T2 group increased (p <0.05) all parameters compared to mice in the C+ group. However, administration of GTE to mice in the T3 group caused a decrease (p <0.05) in all parameters than those of the T2 group and was not significantly different (p >0.05) compared to those of the C+ group. It could be concluded that the administration of GTE at 40 mg/kg BW has maintained the spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice exposed to cigarette smoke.

show abstract

Section: Discussionmentioning

confidence: 99%

Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke

Silviani¹,

Sukmanadi²,

Kurnijasanti³

et al. 2022

Ovz

View full text Add to dashboard Cite

show abstract

“…The thawed sperm membrane integrity was evaluated by a hypo-osmotic swelling test (HOST) [ 22 ]. A 20 µL aliquot of thawed semen was diluted in 200 µL of hypo-osmotic solution (9 mg/mL fructose and 4.9 mg/mL sodium citrate).…”

Section: Methodsmentioning

confidence: 99%

Effects of Different Diluents and Freezing Methods on Cryopreservation of Hu Ram Semen

Zhang,

Wang,

Jiang

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

The purpose of this study was to investigate the effects of different diluents and freezing methods on the quality of thawed sperm after cryopreservation and find an inexpensive and practical method for freezing Hu ram semen for use in inseminations under farm conditions. Ejaculates were collected from five Hu rams. In experiment I, ejaculates were diluted with eight different freezing diluents (basic diluents A, B, C, D, E, F, G, and H). After dilution and cooling, the samples were loaded into 0.25 mL straws and frozen using the liquid nitrogen fumigation method. In experiment II, diluent C was used as the basic diluent and the semen was frozen using liquid nitrogen fumigation and two program-controlled cooling methods. For analysis, frozen samples were evaluated in terms of motility parameters (total motility (TM), progressive motility (PM)), biokinetic characteristics (straight-line velocity (VSL), average path velocity (VAP), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), wobble movement coefficient (WOB), average motion degree (MAD)), reactive oxygen species (ROS) level, and membrane and acrosome integrity. In experiment I, diluent C had higher TM, PM, and acrosome and membrane integrity and lower ROS compared to other extenders (p < 0.05) except diluent A. Diluent C exhibited higher (p < 0.05) VCL, VAP, ALH, WOB, and MAD compared to diluents B, D, E, and F. In experiment II, TM and all biokinetic characteristics did not show significant differences (p > 0.05) amongst the three freezing methods. Liquid nitrogen fumigation resulted in higher (p < 0.05) PM, membrane integrity, acrosome integrity, and lower ROS level compared to the program. In conclusion, the thawed semen diluted with diluent C had higher quality compared to other diluents. The liquid nitrogen fumigation demonstrated superior semen cryopreservation effects compared to the program-controlled cooling method using diluent C.

show abstract

“…Hypo-osmotic swelling test (HOST) was used to measure the sperm plasma membrane integrity [65]. Briefly, 200 µL of the semen was mixed with 1 mL of HOST solution and incubated at 37 • C for 30 min.…”

Section: Detection Of Sperm Plasma Membrane Integritymentioning

confidence: 99%

Phenylbutyrate and Dichloroacetate Enhance the Liquid-Stored Boar Sperm Quality via PDK1 and PDK3

Guo,

Zhang,

Huang

et al. 2023

IJMS

View full text Add to dashboard Cite

Artificial insemination (AI) with liquid-stored semen is the most prevalent and efficient assisted reproduction technique in the modern pork industry. Pyruvate dehydrogenase complex component X (PDHX) was demonstrated to be associated with sperm metabolism and affected the boar sperm viability, motility, and fertility. Pyruvate Dehydrogenase Kinases (PDKs) are the key metabolic enzymes that regulate pyruvate dehydrogenase complex (PDHC) activity and also the conversion from glycolysis to oxidative phosphorylation. In the present study, two PDK inhibitors, Dichloroacetate (DCA) and Phenylbutyrate (4-PBA), were added to an extender and investigated to determine their regulatory roles in liquid-stored boar sperm at 17 °C. The results indicated that PDK1 and PDK3 were predominantly located at the head and flagella of the boar sperm. The addition of 2 mM DCA and 0.5 mM 4-PBA significantly enhanced the sperm motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and ATP content. In addition, DCA and 4-PBA exerted their effects by inhibiting PDK1 and PDK3, respectively. In conclusion, DCA and 4-PBA were found to regulate the boar sperm metabolic activities via PDK1 and PDK3. These both can improve the quality parameters of liquid-stored boar sperm, which will help to improve and optimize liquid-stored boar semen after their addition in the extender.

show abstract

Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology

Cited by 12 publications

References 27 publications

Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke

Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke

Effects of Different Diluents and Freezing Methods on Cryopreservation of Hu Ram Semen

Phenylbutyrate and Dichloroacetate Enhance the Liquid-Stored Boar Sperm Quality via PDK1 and PDK3

Contact Info

Product

Resources

About