Hypoosmotic shock adaptation by prolactin involves upregulation of arginine vasotocin and osmotic stress transcription factor 1 mRNA in the cinnamon clownfish<i>Amphiprion melanopus</i>

Park, Mi Seon; Kim, Na Na; Shin, Hyun Suk; Min, Byung Hwa; Kil, Gyung‐Suk; Cho, Sung Hwoan; Choi, Cheol Young

doi:10.1080/19768354.2012.719547

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…The sequence of the full-length IT gene and its neighboring regions was obtained by DNA walking, as described previously [23], using genomic DNA isolated from clownfish muscle and cDNA pre- precursor [24] of A. melanopus (AEB00559). The phylogenetic tree also indicated similarity between cinnamon clownfish and other teleosts, but a distant separation from non-teleost ITs (Fig.…”

Section: Resultsmentioning

confidence: 99%

Investigation of the Gene Encoding Isotocin and its Expression in Cinnamon Clownfish, Amphiprion melanopus

Noh¹,

Choi²,

Min³

et al. 2016

Journal of Life Science

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Isotocin (IT), a nonapeptide homolog of oxytocin in mammals, has been suggested to be involved in physiological processes including social behaviors, stress responses, and osmoregulation in teleost fish. To study its structure and function, the gene encoding the IT precursor was cloned from the genomic DNA and brain cDNA of the cinnamon clownfish, Amphiprion melanopus. The IT precursor gene consists of three exons separated by two introns, and encodes an open reading frame of 156 amino acid (aa) residues, comprising a putative signal peptide of 19 aa, a mature IT protein of 9 aa, a proteolytic processing site of 3 aa, and 125 aa of neurophysin. Tissue-specific analysis of the IT precursor transcript indicated its expression in the brain and gonads of A. melanopus. To examine its osmoregulatory effects, the salinity of the seawater (34 ppt) used for rearing A. melanopus was lowered to 15 ppt. Histological analysis of the gills indicated the apparent disappearance of an apical crypt on the surface of the gill lamella of A. melanopus, as pavement cells covered the surface upon acclimation to the lower salinity. The level of NaATPase activity in the gills was increased during the initial stage of acclimation, followed by a decrease to its normal level, suggesting its involvement in osmoregulation and homeostasis. The only slight increase in the level of IT precursor transcript in the A. melanopus brain upon low-salinity acclimation suggested that IT played a minor role, if any, in the process of osmoregulation.

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Section: Resultsmentioning

confidence: 99%

Investigation of the Gene Encoding Isotocin and its Expression in Cinnamon Clownfish, Amphiprion melanopus

Noh¹,

Choi²,

Min³

et al. 2016

Journal of Life Science

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“…Their organs that are involved in osmoregulation, such as the gills, kidneys, and liver, have proteins and hormones that regulate ions and water so these fish can adapt to the local environment. The hormones and proteins involved in osmoregulation include aquaporins (AQPs), cortisol, prolactin, growth hormone, Na + /K + -ATPase (NKA), and arginine vasotocin (Matsuzaki et al 2002;Marshall et al 2005;Park et al 2012;Sakamoto and McCormick 2006).…”

Section: Introductionmentioning

confidence: 99%

Effects of Recombinant Aquaporin 3 and Seawater Acclimation on the Expression of Aquaporin 3 and 8 mRNAs in the Parr and Smolt Stages of Rainbow Trout, Oncorhynchus mykiss

Kim¹,

Choi²,

Lim³

et al. 2016

Ocean and Polar Research

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: This study aimed to examine the role of two aquaporin isoforms (AQP3 and AQP8) in response to the hyperosmotic challenge of transitioning from freshwater (FW) to seawater (SW) during parr and smoltification (smolt) using the rainbow trout, Oncorhynchus mykiss. We examined the changes in the expression of AQPs mRNAs in the gills and intestine of the parr and smolt stages of rainbow trout transferred from FW to SW using quantitative real-time PCR in an osmotically changing environment [FW, SW, and recombinant AQP3 (rAQP3) injection at two dosage rates]. Correspondingly, AQPs were greater during smoltification than during parr stages in the rainbow trout. Plasma osmolality and gill Na + /K + -ATPase activity increased when the fish were exposed to SW, but these parameters decreased when the fish were exposed to SW following treatment with rAQP3 during the transition to seawater. Our results suggest that AQPs play an important role in water absorbing mechanisms associated with multiple AQP isoforms in a hyperosmotic environment.

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Dynamic expression of vasotocin and isotocin receptor genes in the marbled eel (Anguilla marmorata) following osmotic challenges

Cao

Liang

Wang

et al. 2018

Gene

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Hypoosmotic shock adaptation by prolactin involves upregulation of arginine vasotocin and osmotic stress transcription factor 1 mRNA in the cinnamon clownfishAmphiprion melanopus

Cited by 4 publications

References 33 publications

Investigation of the Gene Encoding Isotocin and its Expression in Cinnamon Clownfish, Amphiprion melanopus

Investigation of the Gene Encoding Isotocin and its Expression in Cinnamon Clownfish, Amphiprion melanopus

Effects of Recombinant Aquaporin 3 and Seawater Acclimation on the Expression of Aquaporin 3 and 8 mRNAs in the Parr and Smolt Stages of Rainbow Trout, Oncorhynchus mykiss

Dynamic expression of vasotocin and isotocin receptor genes in the marbled eel (Anguilla marmorata) following osmotic challenges

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