Hypothalamic Galanin-producing neurons regulate stress in zebrafish through a peptidergic, self-inhibitory loop

Corradi, Laura; Bruzzone, Matteo; Maschio, Marco Dal; Sawamiphak, Suphansa; Filosa, Alessandro

doi:10.1016/j.cub.2022.02.011

Cited by 15 publications

(34 citation statements)

References 74 publications

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“…Thus, we dissect these 2 mental disorders into simpler behavioral markers (Table 2) by considering their symptoms and diagnosis criteria. We also classify the endophenotypes into four broad categories: (1) Activity and Marco et al, 2013Irons et al, 2013;Woods et al, 2014;Vom Berg-Maurer et al, 2016;Barrios et al, 2020;Corradi et al, 2022) Sleep dysregulation Sleep initiation/sleep maintenance (arousal threshold)/sleep Medium Multiple (Prober et al, 2006;Chiu et al, 2016;Rihel et al, 2010) Reduced arousal threshold Light-dark transition Medium Monoamines, HPA (De Marco et al, 2013Irons et al, 2013;Sveinsdóttir et al, 2022) Flight or freeze Freezing/immobility Freezing Medium Left dorsal habenulo-interpeduncular pathway (Duboué et al, 2017) Increased startle responsiveness…”

Section: Human and Zebrafish Anxiety And Mood Endophenotypesmentioning

confidence: 99%

“…The ablation of these neurons resulted in significant stress-induced hyperactivity, suggesting the galanin-producing neurons are involved in the inhibition of the stress response. The HPA axis was also more active in larvae with ablated galanin-producing neurons, as indicated by higher cortisol levels (Corradi et al, 2022). In mammals, the neuropeptide galanin similarly regulates the stress response, particularly via the HPA axis to prevent overactivation which is often the case in stress-related anxiety and mood disorders (Karlsson and Holmes, 2006;Picciotto et al, 2010;Juhasz et al, 2014).…”

Section: Locomotionmentioning

confidence: 99%

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Larval Zebrafish as a Model for Mechanistic Discovery in Mental Health

Tan

Ang

Wee

2022

Front. Mol. Neurosci.

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Animal models are essential for the discovery of mechanisms and treatments for neuropsychiatric disorders. However, complex mental health disorders such as depression and anxiety are difficult to fully recapitulate in these models. Borrowing from the field of psychiatric genetics, we reiterate the framework of ‘endophenotypes’ – biological or behavioral markers with cellular, molecular or genetic underpinnings – to reduce complex disorders into measurable behaviors that can be compared across organisms. Zebrafish are popular disease models due to the conserved genetic, physiological and anatomical pathways between zebrafish and humans. Adult zebrafish, which display more sophisticated behaviors and cognition, have long been used to model psychiatric disorders. However, larvae (up to 1 month old) are more numerous and also optically transparent, and hence are particularly suited for high-throughput screening and brain-wide neural circuit imaging. A number of behavioral assays have been developed to quantify neuropsychiatric phenomena in larval zebrafish. Here, we will review these assays and the current knowledge regarding the underlying mechanisms of their behavioral readouts. We will also discuss the existing evidence linking larval zebrafish behavior to specific human behavioral traits and how the endophenotype framework can be applied. Importantly, many of the endophenotypes we review do not solely define a diseased state but could manifest as a spectrum across the general population. As such, we make the case for larval zebrafish as a promising model for extending our understanding of population mental health, and for identifying novel therapeutics and interventions with broad impact.

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Section: Human and Zebrafish Anxiety And Mood Endophenotypesmentioning

confidence: 99%

Section: Locomotionmentioning

confidence: 99%

Larval Zebrafish as a Model for Mechanistic Discovery in Mental Health

Tan

Ang

Wee

2022

Front. Mol. Neurosci.

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“…Because RS techniques can be analyzed cause-by-cause, direct information from satellite imagery, such as parameters, can be used to develop flood forecasting scenarios. Through the combined use of satellite or air sensor systems, the geographic distribution of land objects is determined, and together with data such as land and slope, it is converted into digital form and stored in GIS systems [15][16].…”

Section: S Technology and Its Integrationmentioning

confidence: 99%

Water Quality Monitoring and Early Warning Technology of Zebrafish Behavior Based on 3s Technology

2021

AJEB

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The development and key technology research of zebrafish online biomonitoring technology can realize real-time monitoring of water quality safety from the perspective of ecosystem by means of 3s technology, and realize early warning of water quality according to the response characteristics of zebrafish. The purpose of this paper is to study the water quality monitoring and early warning technology of zebrafish behavior based on 3s technology. Use GIS technology to process a large number of graphics, data and models, combine graphics and mathematical models, and implement the results of operations on the map. By analyzing the factors that affect the behavior of the indicator biological zebrafish, the results show that these potential physical stress factors have an impact on the normal behavior of zebrafish, but they are also acceptable in the normal range. Zebrafish can adjust and adapt within the fluctuation range of normal water quality, such as pH value and dissolved oxygen, and the behavioral difference is not obvious.

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“…This approach takes advantage of the phosphorylation of the extracellular signal-regulated kinase (ERK) upon neuronal activation. For complete details on the use and execution of this protocol, please refer to Corradi et al. (2022) .…”

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Corradi et al. (2022) .…”

mentioning

confidence: 99%

Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae

Corradi¹,

Zaupa²,

Sawamiphak³

et al. 2022

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Hypothalamic Galanin-producing neurons regulate stress in zebrafish through a peptidergic, self-inhibitory loop

Cited by 15 publications

References 74 publications

Larval Zebrafish as a Model for Mechanistic Discovery in Mental Health

Larval Zebrafish as a Model for Mechanistic Discovery in Mental Health

Water Quality Monitoring and Early Warning Technology of Zebrafish Behavior Based on 3s Technology

Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae

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