Hypoxanthine and xanthine levels determined by high-performance liquid chromatography in plasma, erythrocyte, and urine samples from healthy subjects: The problem of hypoxanthine level evolution as a function of time

Boulieu, Roselyne; Bory, C.; Baltassat, P; Gonnet, C.

doi:10.1016/0003-2697(83)90568-7

Cited by 77 publications

(39 citation statements)

References 14 publications

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“…μmol/L RBCs (Boulieu et al 1983;Werner et al 1987 (Tavazzi et al 2000). In addition, the present basal AEC suggest that the current process provided a stable metabolic state in RBCs because the decreased energy charge was associated with the degradation of nucleotides (Atkinson 1968;Matsumoto et al 1979;de Atauri et al 2006).…”

Section: Discussionmentioning

confidence: 89%

A Novel Method for Measuring the ATP-Related Compounds in Human Erythrocytes

Aragón-Martínez

Galicia-Castillo

Isiordia-Espinoza

et al. 2014

Tohoku J. Exp. Med.

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The ATP-related compounds in whole blood or red blood cells have been used to evaluate the energy status of erythrocytes and the degradation level of the phosphorylated compounds under various conditions, such as chronic renal failure, drug monitoring, cancer, exposure to environmental toxics, and organ preservation. The complete interpretation of the energetic homeostasis of erythrocytes is only performed using the compounds involved in the degradation pathway for adenine nucleotides alongside the uric acid value. For the first time, we report a liquid chromatographic method using a diode array detector that measures all of these compounds in a small human whole blood sample (125 μL) within an acceptable time of 20 min. The stability was evaluated for all of the compounds and ranged from 96.3 to 105.1% versus the day zero values. The measurement had an adequate sensitivity for the ATP-related compounds (detection limits from 0.001 to 0.097 μmol/L and quantification limits from 0.004 to 0.294 μmol/L). This method is particularly useful for measuring inosine monophosphate, inosine, hypoxanthine, and uric acid. Moreover, this assay had acceptable linearity (r > 0.990), precision (coefficients of variation ranged from 0.1 to 2.0%), specificity (similar retention times and spectra in all samples) and recoveries (ranged from 89.2 to 104.9%). The newly developed method is invaluable for assessing the energetic homeostasis of red blood cells under diverse conditions, such as in vitro experiments and clinical settings.

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Section: Discussionmentioning

confidence: 89%

A Novel Method for Measuring the ATP-Related Compounds in Human Erythrocytes

Aragón-Martínez

Galicia-Castillo

Isiordia-Espinoza

et al. 2014

Tohoku J. Exp. Med.

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“…Allopurinol inhibits metabolism of xanthine to uric acid. Recombinant urate oxidase catalyzes the metabolism of uric acid into the more soluble allantoin (55,94,95). Solubilities at a pH of 7 are shown in parentheses.…”

Section: Phosphorous and Calciummentioning

confidence: 99%

Onco-Nephrology

Wilson

Berns

2012

Clinical Journal of the American Society of Nephrology

123

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SummaryTumor lysis syndrome (TLS) describes the clinical and laboratory sequelae that result from the rapid release of intracellular contents of dying cancer cells. It is characterized by the release of potassium, phosphorous, and nucleic acids from cancer cells into the blood stream, with the potential to cause hyperkalemia; hyperphosphatemia and secondary hypocalcemia; hyperuricemia; AKI; and, should usual homeostatic mechanisms fail, death. TLS most commonly follows treatment of hematologic malignancies, such as acute lymphocytic or lymphoblastic leukemia, acute myeloid leukemia, and Burkitt lymphoma, but also occurs after treatment of other bulky or rapidly growing tumors, particularly if the patient is highly sensitive to the effects of cytotoxic chemotherapy. Prevention and treatment depend on prompt recognition of patients at risk, volume repletion, allopurinol, rasburicase (a novel recombinant urate oxidase), and, when indicated, dialysis.

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“…The plasma hypoxanthine level was elevated at 0.095 mg/dL (normal range 0.014-0.061 mg/dL [8]). The plasma xanthine level was also elevated at 0.390 mg/dL (normal range 0-0.038 mg/dL [8]). After allopurinol administration (300 mg/body) [9], her plasma oxypurinol levels were increased to 0.614 mg/dL (Table 1).…”

Section: Allopurinol Loading Testmentioning

confidence: 99%

“…Plasma and urinary levels of hypoxanthine and xanthine were measured by high-pressure liquid chromatography [4]. The plasma hypoxanthine level was elevated at 0.095 mg/dL (normal range 0.014-0.061 mg/dL [8]). The plasma xanthine level was also elevated at 0.390 mg/dL (normal range 0-0.038 mg/dL [8]).…”

Section: Allopurinol Loading Testmentioning

confidence: 99%

A case of xanthinuria type I with a novel mutation in xanthine dehydrogenase

et al. 2016

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Hereditary hypouricemia is generally caused by renal hypouricemia, an autosomal recessive disorder that is characterized by impaired renal tubular uric acid transport, or by xanthinuria, a rare autosomal recessive disorder caused by a deficiency of xanthine dehydrogenase (XDH; xanthinuria type I) or by a deficiency of both XDH and aldehyde oxidase (xanthinuria type II). In contrast to renal hypouricemia, which sometimes leads to exercise-induced acute kidney injury (EIAKI), xanthinuria has not been associated with this disorder. We report here a case of xanthinuria type I due to a compound heterozygous mutation. A 46-year-old woman was found to have undetectable plasma and urinary levels of uric acid. She had no symptoms and no history of EIAKI. Xanthinuria type I was diagnosed following the allopurinol loading test. Mutation analysis revealed a compound heterozygous mutation [c.305A[G (p.Gln102Arg) and c.2567delC (p.Thr856Lysfs*73)] in the XDH gene. Of these two mutations, the former is novel. The patient did not exhibit EIAKI. However, because xanthinuria is a rare disease, the identification of additional cases is necessary to determine whether this disease is complicated with EIAKI.

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Hypoxanthine and xanthine levels determined by high-performance liquid chromatography in plasma, erythrocyte, and urine samples from healthy subjects: The problem of hypoxanthine level evolution as a function of time

Cited by 77 publications

References 14 publications

A Novel Method for Measuring the ATP-Related Compounds in Human Erythrocytes

A Novel Method for Measuring the ATP-Related Compounds in Human Erythrocytes

Onco-Nephrology

A case of xanthinuria type I with a novel mutation in xanthine dehydrogenase

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