Hypoxia related long non-coding RNAs in ischemic stroke

Yao, Jiawei; Du, Yiqun; Liu, Junsi; Gareev, Ilgiz; Yang, Guang; Kang, Xiaohui; Wang, Xiaoxiong; Beylerli, Ozal; Chen, Xin

doi:10.1016/j.ncrna.2021.10.001

Cited by 6 publications

(3 citation statements)

References 84 publications

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“…Long noncoding RNAs (lncRNAs), novel noncoding RNAs with a length of over 200 nucleotides, play an essential part in inflammatory responses and diseases [ 19 ]. Reportedly, hypoxia and ischemia in the aftermath of lessened or interrupted cerebral blood flow can bring about alterations in the profiles of many lncRNAs in brain tissues, hence influencing disease progression [ 20 – 23 ]. lncRNA MIR497HG (MIR497HG), a member of the lncRNA family, targets the miRNA-128-3p/SIRT1 axis to suppress proliferation and migration in human retinal endothelial cells elicited by high glucose [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis

et al. 2022

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Background. Chlorogenic acid (CGA) is a polyphenolic compound with antioxidant and anti-inflammatory properties. CGA has been shown to improve neuroinflammation. This study is aimed at elucidating the exact mechanism by which CGA reduces neuroinflammation. Methods. Oxygen and glucose deprivation (OGD) was utilized to treat BV2 microglia and HT-22 hippocampal neurons to engineer an in vitro model of hypoxic ischemia reperfusion. The levels of inflammatory factors (IL-1β, IL-6, TNF-α, IL-4, and IL-10) and oxidative stress factors (MDA, SOD, and GSH-PX) in microglia were determined by ELISA kits. The neuron proliferation was assessed by CCK-8 assay, and LDH kit was used to determine LDH release in neurons. The fluorescent dye DCF-DA was employed to measure ROS levels in neurons. Correlation of MIR497HG, miR-29b-3p, and SIRT1/NF-κB in neurons and microglia was determined by qRT-PCR. Expressions of inflammatory proteins (COX2, iNOS), oxidative stress pathways (Nrf2, HO-1), and apoptosis-related proteins (Bcl-2, Bax, caspase3, caspase8, and caspase9) in microglia or neurons were determined by western blot. The interactions between MIR497HG and miR-29b-3p, as well as between miR-29b-3p and SIRT1, were determined by dual luciferase assay and RIP assay. Results. CGA attenuated OGD-mediated inflammation and oxidative stress in microglia and inhibited microglia-mediated neuronal apoptosis. CGA increased the levels of MIR497HG and SIRT1 and suppressed the levels of miR-29b-3p in BV2 and HT-22 cells. MIR497HG knockdown, miR-29b-3p upregulation, and SIRT1 inhibition inhibited CGA-mediated anti-inflammatory and neuronal protective functions. There is a targeting correlation between MIR497HG, miR-29b-3p, and Sirt1. MIR497HG sponges miR-29b-3p to regulate SIRT1 expression in an indirect manner. Conclusion. CGA upregulates MIR497HG to curb miR-29b-3p expression, hence initiating the SIRT1/NF-κB signaling pathway and repressing OGD-elicited inflammation, oxidative stress, and neuron apoptosis.

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Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis

et al. 2022

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“…It was previously found that the immune response of monocytes and the replication of the COVID-19 virus require the support of aerobic glycolysis [ 136 ]. The transcription factor hypoxia-inducible factor 1-alpha (HIF-1a) can induce glycolysis, leading to a pro-inflammatory state in monocytes infected with COVID-19 [ 137 ]. It has also been previously found that HIF1A stabilizing long non-coding RNA (HISLA) in macrophages provides stability to HIF-1a by inhibiting its binding to the prolyl hydroxylase domain-containing protein 2 (PHD2) protein, thereby preventing its degradation and thus maintaining continuous activation of the aerobic state in tumor cells through HIF-1a signaling [ 138 ].…”

Section: Non-coding Rnas In Covid-19mentioning

confidence: 99%

COVID-19: Mechanisms, risk factors, genetics, non-coding RNAs and neurologic impairments

Gilyazova

Timasheva

Карунас

et al. 2023

Non-coding RNA Research

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“…They also act as structural components in protein complex assembly and serve as competing endogenous RNAs (ceRNAs) to modulate miRNA functions [23,30]. In this regard, lncRNAs can inhibit or activate target genes, by directly binding to them or by recruiting transcription factors and thus modulating many cellular processes, including cell proliferation, differentiation, apoptosis, autophagy, angiogenesis, and immune response [31,32].…”

Section: Introductionmentioning

confidence: 99%

LncRNAs and CircRNAs as Strategies against Pathological Conditions Caused by a Hypoxic/Anoxic State

Anchesi,

Schepici,

Mazzon

2023

Biomolecules

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Brain damage can be induced by oxygen deprivation. It is known that hypoxic or anoxic conditions can lead to changes in the expression levels of non-coding RNAs (ncRNAs), which, in turn, can be related to Central Nervous System (CNS) injuries. Therefore, it could be useful to investigate the involvement of non-coding RNAs (ncRNAs), as well as the underlying mechanisms which are able to modulate them in brain damage induced by hypoxic or anoxic conditions. In this review, we focused on recent research that associates these conditions with long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs). The results of this review demonstrate that the expression of both lncRNAs and circRNAs can be influenced by oxygen deprivation conditions and so they can contribute to inducing damage or providing neuroprotection by affecting specific molecular pathways. Furthermore, several experimental studies have shown that ncRNA activity can be regulated by compounds, thus also modifying their transcriptomic profile and their effects on CNS damages induced by hypoxic/anoxic events.

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Hypoxia related long non-coding RNAs in ischemic stroke

Cited by 6 publications

References 84 publications

Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis

Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis

COVID-19: Mechanisms, risk factors, genetics, non-coding RNAs and neurologic impairments

LncRNAs and CircRNAs as Strategies against Pathological Conditions Caused by a Hypoxic/Anoxic State

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