Bacillus subtilis BS-2 and Peppermint Oil as Biocontrol Agents Against Botrytis cinerea

Abstract

The purpose of this study was to assess the activity of Bacillus subtilis BS-2 and peppermint oil against Botrytis cinerea. In this study parameters such as the age and the density of the bacterial culture and the incubation temperature were taken into consideration. Furthermore, the cellulolytic activity of the bacterium was determined. The effect of peppermint oil was evaluated at a concentration range of 0.5-4.0 %. The research was conducted with a dual culture plate method. The influence of B. subtilis BS-2 and peppermint oil on the growth of B. cinerea was evaluated based on the growth rate index. It was noted that the bacterial culture occurred at an initial density of OD560 = 1.0, cultivated at 30 °C for 48 hours demonstrated the strongest antagonistic effect (57.07 % inhibition). Furthermore, it was observed that the highest cellulolytic activity occurred on the bacteria incubated for 48 hours at 37 °C. The effect of mint oil, at the lowest concentration of 0.5-1.0 %, was much weaker on bacterial activity (1.1-12.1 % inhibition). The highest concentration (4.0 %) of mint oil caused the maximum inhibition (31.9 %) of the mycelial growth. B. subtilis BS-2 may be environmental-friendly alternatives for protecting plants against B. cinerea

Molecular disparities among Botrytis species involved in onion umbel blight disease and its management using Bacillus subtilis PHYS7

“…In accordance with our results, Botrytis spp. the causal pathogens of BOUB disease, was managed biologically B. subtilis as a good antagonistic microorganism was reported by its variable mechanisms of action against many fungal pathogens (Alamri et al, 2019;Punja et al, 2019 andGrata et al, 2019). B. subtilis produces bacteriocins that play an important role in essential host immunity .…”